ID   UD73_DORVU              Reviewed;          35 AA.
AC   P0DUT4;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=U-limacoditoxin(13)-Dv73 {ECO:0000303|PubMed:33893140};
DE            Short=U-LCTX(13)-Dv73 {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Cecropin-like peptide {ECO:0000303|PubMed:33893140};
DE   AltName: Full=Vulnericin {ECO:0000303|PubMed:33893140};
DE   Flags: Precursor;
OS   Doratifera vulnerans (Mottled cup moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Zygaenoidea;
OC   Limacodidae; Doratifera.
OX   NCBI_TaxID=1372962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-34, FUNCTION,
RP   SUBCELLULAR LOCATION, AMIDATION AT LEU-34, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=33893140; DOI=10.1073/pnas.2023815118;
RA   Walker A.A., Robinson S.D., Paluzzi J.V., Merritt D.J., Nixon S.A.,
RA   Schroeder C.I., Jin J., Goudarzi M.H., Kotze A.C., Dekan Z., Sombke A.,
RA   Alewood P.F., Fry B.G., Epstein M.E., Vetter I., King G.F.;
RT   "Production, composition, and mode of action of the painful defensive venom
RT   produced by a limacodid caterpillar, Doratifera vulnerans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC   -!- FUNCTION: Probable toxin. Shows a moderate antiparasitic activity
CC       against the major pathogenic nematode of ruminants (H.contortus,
CC       EC(50)=94.5 uM). Does not show insecticidal and antimicrobial
CC       activities. Does not induce increase in intracellular calcium in mouse
CC       DRG neurons, suggesting that it does not induce pain.
CC       {ECO:0000269|PubMed:33893140}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33893140}.
CC   -!- TISSUE SPECIFICITY: Expressed by the spine venom secretory cell. The
CC       spine is a cuticular structure containing at its base a single large
CC       nucleated venom secretory cell, as well as a central venom reservoir
CC       extending throughout the spine. It is an independent unit capable of
CC       producing, storing, and injecting venom. Spines are grouped by 50 to
CC       100 in each of the eight venom scoli on the back of D.vulnerans
CC       caterpillars. {ECO:0000269|PubMed:33893140}.
CC   -!- DEVELOPMENTAL STAGE: Only secreted by caterpillars. Adult moth do not
CC       have spines. {ECO:0000269|PubMed:33893140}.
CC   -!- SIMILARITY: Belongs to the limacoditoxin-13 family. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT   PEPTIDE         21..34
FT                   /note="U-limacoditoxin(13)-Dv73"
FT                   /evidence="ECO:0000269|PubMed:33893140"
FT                   /id="PRO_0000453415"
FT   MOD_RES         34
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:33893140"
SQ   SEQUENCE   35 AA;  3689 MW;  5E28D6CBFC71F6EF CRC64;
     MPKSLLLVLA LALLAQTALA NGPQGSSDRN WIRLG