UDB10_HUMAN
ID UDB10_HUMAN Reviewed; 528 AA.
AC P36537; A8K9M3; B4DPP1; Q14CR8;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=UDP-glucuronosyltransferase 2B10;
DE Short=UDPGT 2B10;
DE EC=2.4.1.17;
DE Flags: Precursor;
GN Name=UGT2B10;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Liver;
RX PubMed=8333863; DOI=10.1006/bbrc.1993.1847;
RA Jin C.-J., Miners J.O., Lillywhite K.J., McKenzie P.I.;
RT "cDNA cloning and expression of two new members of the human liver UDP-
RT glucuronosyltransferase 2B subfamily.";
RL Biochem. Biophys. Res. Commun. 194:496-503(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Liver;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-314.
RC TISSUE=Plasma;
RX PubMed=16335952; DOI=10.1021/pr0502065;
RA Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J.,
RA Smith R.D.;
RT "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT hydrazide chemistry, and mass spectrometry.";
RL J. Proteome Res. 4:2070-2080(2005).
RN [7]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-314.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
CC -!- FUNCTION: UDPGT is of major importance in the conjugation and
CC subsequent elimination of potentially toxic xenobiotics and endogenous
CC compounds.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor
CC beta-D-glucuronoside + H(+) + UDP; Xref=Rhea:RHEA:21032,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58052, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:132367, ChEBI:CHEBI:132368; EC=2.4.1.17;
CC -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}. Endoplasmic reticulum membrane
CC {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P36537-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P36537-2; Sequence=VSP_054337;
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; X63359; CAA44961.1; -; mRNA.
DR EMBL; AK292738; BAF85427.1; -; mRNA.
DR EMBL; AK298432; BAG60653.1; -; mRNA.
DR EMBL; AC021146; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471057; EAX05577.1; -; Genomic_DNA.
DR EMBL; BC113649; AAI13650.1; -; mRNA.
DR CCDS; CCDS75135.1; -. [P36537-2]
DR CCDS; CCDS75136.1; -. [P36537-1]
DR PIR; JN0620; JN0620.
DR RefSeq; NP_001066.1; NM_001075.5. [P36537-1]
DR RefSeq; NP_001138239.1; NM_001144767.2. [P36537-2]
DR RefSeq; NP_001277020.1; NM_001290091.1.
DR AlphaFoldDB; P36537; -.
DR SMR; P36537; -.
DR BioGRID; 113212; 3.
DR IntAct; P36537; 1.
DR STRING; 9606.ENSP00000265403; -.
DR BindingDB; P36537; -.
DR ChEMBL; CHEMBL6160; -.
DR DrugBank; DB00321; Amitriptyline.
DR DrugBank; DB00924; Cyclobenzaprine.
DR DrugBank; DB00434; Cyproheptadine.
DR DrugBank; DB00920; Ketotifen.
DR DrugBank; DB00455; Loratadine.
DR DrugBank; DB11837; Osilodrostat.
DR DrugBank; DB06153; Pizotifen.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR GlyGen; P36537; 3 sites.
DR iPTMnet; P36537; -.
DR PhosphoSitePlus; P36537; -.
DR BioMuta; UGT2B10; -.
DR DMDM; 549155; -.
DR jPOST; P36537; -.
DR MassIVE; P36537; -.
DR MaxQB; P36537; -.
DR PaxDb; P36537; -.
DR PeptideAtlas; P36537; -.
DR PRIDE; P36537; -.
DR ProteomicsDB; 4800; -.
DR ProteomicsDB; 55206; -. [P36537-1]
DR Antibodypedia; 24201; 130 antibodies from 23 providers.
DR DNASU; 7365; -.
DR Ensembl; ENST00000265403.12; ENSP00000265403.7; ENSG00000109181.12. [P36537-1]
DR Ensembl; ENST00000458688.2; ENSP00000413420.2; ENSG00000109181.12. [P36537-2]
DR Ensembl; ENST00000615213.3; ENSP00000478760.1; ENSG00000275190.3. [P36537-1]
DR Ensembl; ENST00000627047.1; ENSP00000486876.1; ENSG00000275190.3. [P36537-2]
DR GeneID; 7365; -.
DR KEGG; hsa:7365; -.
DR MANE-Select; ENST00000265403.12; ENSP00000265403.7; NM_001075.6; NP_001066.1.
DR UCSC; uc003hee.4; human. [P36537-1]
DR CTD; 7365; -.
DR DisGeNET; 7365; -.
DR GeneCards; UGT2B10; -.
DR HGNC; HGNC:12544; UGT2B10.
DR HPA; ENSG00000109181; Tissue enriched (liver).
DR MIM; 600070; gene.
DR neXtProt; NX_P36537; -.
DR OpenTargets; ENSG00000109181; -.
DR PharmGKB; PA37186; -.
DR VEuPathDB; HostDB:ENSG00000109181; -.
DR eggNOG; KOG1192; Eukaryota.
DR GeneTree; ENSGT00940000165281; -.
DR HOGENOM; CLU_012949_3_0_1; -.
DR InParanoid; P36537; -.
DR OMA; KVRHMET; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; P36537; -.
DR TreeFam; TF315472; -.
DR BRENDA; 2.4.1.17; 2681.
DR PathwayCommons; P36537; -.
DR Reactome; R-HSA-156588; Glucuronidation.
DR Reactome; R-HSA-9749641; Aspirin ADME.
DR SignaLink; P36537; -.
DR BioGRID-ORCS; 7365; 11 hits in 233 CRISPR screens.
DR ChiTaRS; UGT2B10; human.
DR GeneWiki; UGT2B10; -.
DR GenomeRNAi; 7365; -.
DR Pharos; P36537; Tbio.
DR PRO; PR:P36537; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; P36537; protein.
DR Bgee; ENSG00000109181; Expressed in liver and 23 other tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0052695; P:cellular glucuronidation; IBA:GO_Central.
DR GO; GO:0008210; P:estrogen metabolic process; IBA:GO_Central.
DR GO; GO:0006629; P:lipid metabolic process; TAS:ProtInc.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endoplasmic reticulum; Glycoprotein;
KW Glycosyltransferase; Membrane; Microsome; Reference proteome; Signal;
KW Transferase; Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000250"
FT CHAIN 24..528
FT /note="UDP-glucuronosyltransferase 2B10"
FT /id="PRO_0000036034"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 134
FT /note="N6-succinyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8BWQ1"
FT CARBOHYD 66
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 314
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:16335952,
FT ECO:0000269|PubMed:19159218"
FT CARBOHYD 481
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 156..239
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054337"
SQ SEQUENCE 528 AA; 60774 MW; 5E6EA3DBC032C2E0 CRC64;
MALKWTTVLL IQLSFYFSSG SCGKVLVWAA EYSLWMNMKT ILKELVQRGH EVTVLASSAS
ILFDPNDSST LKLEVYPTSL TKTEFENIIM QLVKRLSEIQ KDTFWLPFSQ EQEILWAIND
IIRNFCKDVV SNKKLMKKLQ ESRFDIVFAD AYLPCGELLA ELFNIPFVYS HSFSPGYSFE
RHSGGFIFPP SYVPVVMSKL SDQMTFMERV KNMLYVLYFD FWFQIFNMKK WDQFYSEVLG
RPTTLSETMR KADIWLMRNS WNFKFPHPFL PNVDFVGGLH CKPAKPLPKE MEEFVQSSGE
NGVVVFSLGS MVSNMTEERA NVIATALAKI PQKVLWRFDG NKPDALGLNT RLYKWIPQND
LLGHPKTRAF ITHGGANGIY EAIYHGIPMV GIPLFFDQPD NIAHMKAKGA AVRVDFNTMS
STDLLNALKT VINDPSYKEN IMKLSRIQHD QPVKPLDRAV FWIEFVMRHK GAKHLRVAAH
NLTWFQYHSL DVIGFLLACV ATVLFIITKC CLFCFWKFAR KGKKGKRD
