ID   UEVLD_DANRE             Reviewed;         471 AA.
AC   Q6DBY5;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 variant 3;
DE            Short=UEV-3;
DE   AltName: Full=EV and lactate/malate dehydrogenase domain-containing protein;
GN   Name=uevld; Synonyms=uev3; ORFNames=zgc:100959;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Possible negative regulator of polyubiquitination.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the ubiquitin-
CC       conjugating enzyme family. UEV subfamily. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the LDH/MDH
CC       superfamily. {ECO:0000305}.
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DR   EMBL; BC078313; AAH78313.1; -; mRNA.
DR   RefSeq; NP_001003619.1; NM_001003619.2.
DR   AlphaFoldDB; Q6DBY5; -.
DR   SMR; Q6DBY5; -.
DR   STRING; 7955.ENSDARP00000048506; -.
DR   PaxDb; Q6DBY5; -.
DR   Ensembl; ENSDART00000048507; ENSDARP00000048506; ENSDARG00000009266.
DR   Ensembl; ENSDART00000189014; ENSDARP00000148667; ENSDARG00000116754.
DR   GeneID; 445225; -.
DR   KEGG; dre:445225; -.
DR   CTD; 55293; -.
DR   ZFIN; ZDB-GENE-040801-138; uevld.
DR   eggNOG; KOG1495; Eukaryota.
DR   eggNOG; KOG2391; Eukaryota.
DR   GeneTree; ENSGT00940000153903; -.
DR   HOGENOM; CLU_039842_0_1_1; -.
DR   InParanoid; Q6DBY5; -.
DR   OMA; GIAVGKH; -.
DR   OrthoDB; 1435538at2759; -.
DR   PhylomeDB; Q6DBY5; -.
DR   TreeFam; TF314963; -.
DR   PRO; PR:Q6DBY5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 25.
DR   Bgee; ENSDARG00000009266; Expressed in early embryo and 24 other tissues.
DR   GO; GO:0000813; C:ESCRT I complex; IBA:GO_Central.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   GO; GO:0008333; P:endosome to lysosome transport; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:InterPro.
DR   Gene3D; 3.10.110.10; -; 1.
DR   Gene3D; 3.90.110.10; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   InterPro; IPR008883; UEV_N.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   Pfam; PF05743; UEV; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   SUPFAM; SSF56327; SSF56327; 1.
DR   PROSITE; PS51322; UEV; 1.
PE   2: Evidence at transcript level;
KW   NAD; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..471
FT                   /note="Ubiquitin-conjugating enzyme E2 variant 3"
FT                   /id="PRO_0000278653"
FT   DOMAIN          2..145
FT                   /note="UEV"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00652"
FT   BINDING         183..211
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   471 AA;  51264 MW;  1794392F4D72DD42 CRC64;
     MDVNSEPVKK VLSKYKFRDV AVEELQKVYR VYPDMKIMAG TYTSSDSLQK DLLKLVGNIP
     VVYQGRSYNI PILLWLLDSF PFTPPICYLR PTSSMVIREG KHVDSKGRIH LPALHNWDHP
     KSSVNALLAE MIGKFEEEPP LGTKSSAHGD TPSNLLDYVS NLTITEGGNR PDQEVKVSVI
     GGGDLGIAAV LSIMAKSCVD KLVLIDIPEN STKGGTMDLE IFSLPKVEVS KDLSASAGSK
     VLVITANAWS DEQSYLSVVQ TNVDMYRGII PRLAQLSPNA VLVIASQPVD VMTHVAWRQS
     HLLPTQVIGV GCNLDSQRLS HIINISLVAN NTGKQAWVIG ELSENKVAVW GNMGPGTDQL
     QALTPVSNST KPLMDRAFEM IKGRGQRSWS VGLSIADITH SIVTNQKKVH SVTTLAEGWG
     GIGSKVFLSL PCILGETGST RLPGVALGSE DEMKLRESVA CQVNLFMQLR L