UEVLD_MOUSE
ID UEVLD_MOUSE Reviewed; 471 AA.
AC Q3U1V6; O08761; Q7TQD3;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Ubiquitin-conjugating enzyme E2 variant 3;
DE Short=UEV-3;
DE AltName: Full=EV and lactate/malate dehydrogenase domain-containing protein;
GN Name=Uevld; Synonyms=Attp, Uev3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-169.
RC TISSUE=Thymus;
RA Burbelo P.D.;
RT "Role of ATTP in signal transduction.";
RL Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Possible negative regulator of polyubiquitination.
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3U1V6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3U1V6-2; Sequence=VSP_023352, VSP_023353;
CC -!- SIMILARITY: In the N-terminal section; belongs to the ubiquitin-
CC conjugating enzyme family. UEV subfamily. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the LDH/MDH
CC superfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB57699.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK154536; BAE32661.1; -; mRNA.
DR EMBL; AK155685; BAE33387.1; -; mRNA.
DR EMBL; BC054796; AAH54796.1; -; mRNA.
DR EMBL; U97571; AAB57699.1; ALT_FRAME; mRNA.
DR CCDS; CCDS39965.1; -. [Q3U1V6-1]
DR RefSeq; NP_001035785.1; NM_001040695.1. [Q3U1V6-1]
DR AlphaFoldDB; Q3U1V6; -.
DR SMR; Q3U1V6; -.
DR BioGRID; 207562; 1.
DR STRING; 10090.ENSMUSP00000091964; -.
DR iPTMnet; Q3U1V6; -.
DR PhosphoSitePlus; Q3U1V6; -.
DR EPD; Q3U1V6; -.
DR MaxQB; Q3U1V6; -.
DR PaxDb; Q3U1V6; -.
DR PeptideAtlas; Q3U1V6; -.
DR PRIDE; Q3U1V6; -.
DR ProteomicsDB; 298122; -. [Q3U1V6-1]
DR ProteomicsDB; 298123; -. [Q3U1V6-2]
DR Antibodypedia; 25122; 148 antibodies from 21 providers.
DR DNASU; 54122; -.
DR Ensembl; ENSMUST00000094398; ENSMUSP00000091964; ENSMUSG00000043262. [Q3U1V6-1]
DR Ensembl; ENSMUST00000207986; ENSMUSP00000146930; ENSMUSG00000043262. [Q3U1V6-2]
DR GeneID; 54122; -.
DR KEGG; mmu:54122; -.
DR UCSC; uc009gzr.1; mouse. [Q3U1V6-1]
DR UCSC; uc009gzs.1; mouse. [Q3U1V6-2]
DR CTD; 55293; -.
DR MGI; MGI:1860490; Uevld.
DR VEuPathDB; HostDB:ENSMUSG00000043262; -.
DR eggNOG; KOG1495; Eukaryota.
DR eggNOG; KOG2391; Eukaryota.
DR GeneTree; ENSGT00940000153903; -.
DR HOGENOM; CLU_039842_0_1_1; -.
DR InParanoid; Q3U1V6; -.
DR OMA; GIAVGKH; -.
DR OrthoDB; 1435538at2759; -.
DR PhylomeDB; Q3U1V6; -.
DR TreeFam; TF314963; -.
DR BioGRID-ORCS; 54122; 5 hits in 71 CRISPR screens.
DR ChiTaRS; Uevld; mouse.
DR PRO; PR:Q3U1V6; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q3U1V6; protein.
DR Bgee; ENSMUSG00000043262; Expressed in saccule of membranous labyrinth and 202 other tissues.
DR ExpressionAtlas; Q3U1V6; baseline and differential.
DR Genevisible; Q3U1V6; MM.
DR GO; GO:0000813; C:ESCRT I complex; IBA:GO_Central.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR GO; GO:0008333; P:endosome to lysosome transport; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR Gene3D; 3.10.110.10; -; 1.
DR Gene3D; 3.90.110.10; -; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR008883; UEV_N.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR Pfam; PF05743; UEV; 1.
DR PRINTS; PR00086; LLDHDRGNASE.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
DR PROSITE; PS51322; UEV; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; NAD; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..471
FT /note="Ubiquitin-conjugating enzyme E2 variant 3"
FT /id="PRO_0000278652"
FT DOMAIN 2..145
FT /note="UEV"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00652"
FT BINDING 191..219
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT VAR_SEQ 239..250
FT /note="DLSASAHSKVVI -> GGDLHSQLFGRF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023352"
FT VAR_SEQ 251..471
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023353"
FT CONFLICT 26
FT /note="Missing (in Ref. 2; AAH54796)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="K -> R (in Ref. 3; AAB57699)"
FT /evidence="ECO:0000305"
FT CONFLICT 166..169
FT /note="VSDI -> LYCQ (in Ref. 3; AAB57699)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 471 AA; 51681 MW; 650A5B9F0B5E5146 CRC64;
MEFDCEGVRR LLGKYKFRDL TVEELKNVSV SFPHFRYSVD TYVFKDTSQK DLLNFTGTIP
VMYQGKTYNI PIRFWILDSH PFAPPICFLK PTANMEISVG KHVDAKGRIY LPYLQNWSHP
KSAIVGLIKE MIAKFQEELP LYSIPSSNEA QQVDLLAYIT KITEGVSDIN SRGWTNHENK
ILNKITVVGS GDLGIACTLA ISAKGIADKL LLLDLSDGMS QGTMDLDIFN LPNVEISKDL
SASAHSKVVI FTANSLGGSE SYLHAVQSNV DMFRALVPAL GHYSQHAVLL VASQPVEIMS
YVTWKLSTFP ATRVVGIGCN LDSQRLQYII TSVLKVQTSG KEVWVVGEQG ENKVCSWSGR
DGVLSPSSQA QLSSRAMELL KVKGQRSWSV GLSVADLVDT IINNKRKVHS VSTLAKGYYG
LDNEVFLSLP CILGTGGVSE VIKTKAGEDT VTGTLQASAS SIHALQQQLE L
