ID   UEVLD_XENTR             Reviewed;         476 AA.
AC   Q66KB7;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2 variant 3;
DE            Short=UEV-3;
DE   AltName: Full=EV and lactate/malate dehydrogenase domain-containing protein;
GN   Name=uevld; Synonyms=uev3;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Possible negative regulator of polyubiquitination.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the ubiquitin-
CC       conjugating enzyme family. UEV subfamily. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the LDH/MDH
CC       superfamily. {ECO:0000305}.
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DR   EMBL; BC080471; AAH80471.1; -; mRNA.
DR   RefSeq; NP_001007958.1; NM_001007957.1.
DR   AlphaFoldDB; Q66KB7; -.
DR   SMR; Q66KB7; -.
DR   PaxDb; Q66KB7; -.
DR   GeneID; 493332; -.
DR   KEGG; xtr:493332; -.
DR   CTD; 55293; -.
DR   Xenbase; XB-GENE-5786365; uevld.
DR   eggNOG; KOG1495; Eukaryota.
DR   eggNOG; KOG2391; Eukaryota.
DR   HOGENOM; CLU_039842_0_1_1; -.
DR   InParanoid; Q66KB7; -.
DR   OMA; GIAVGKH; -.
DR   OrthoDB; 1435538at2759; -.
DR   PhylomeDB; Q66KB7; -.
DR   TreeFam; TF314963; -.
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0000813; C:ESCRT I complex; IBA:GO_Central.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   GO; GO:0008333; P:endosome to lysosome transport; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:InterPro.
DR   Gene3D; 3.10.110.10; -; 1.
DR   Gene3D; 3.90.110.10; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   InterPro; IPR008883; UEV_N.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   Pfam; PF05743; UEV; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF54495; SSF54495; 1.
DR   SUPFAM; SSF56327; SSF56327; 1.
DR   PROSITE; PS51322; UEV; 1.
PE   2: Evidence at transcript level;
KW   NAD; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..476
FT                   /note="Ubiquitin-conjugating enzyme E2 variant 3"
FT                   /id="PRO_0000278654"
FT   DOMAIN          2..145
FT                   /note="UEV"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00652"
FT   BINDING         185..213
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   476 AA;  51777 MW;  3EF268655C61D34F CRC64;
     MEFSAETLRQ QLGKYKFRDL TIEELKDLNR TFPSFIFSME TYTFRDGSQK DLLNLTGTVP
     MKHQGTTYNI PICLWILDSH PFAPPLCFLK PSGNMGIRVG RHIDAQGRIY LPYLQNWSHP
     KSTVTGLIRE MAVKFEEELP LYSLSAEDGA RQRELLSYIA QVTDGVSSMD VKAPSRAKVT
     VIGGGDMALA CLLAVSAKGT AGKLLLLDPT DGEAAGGAAA DLEIFSLPNV QVTKDFSAIA
     GSAIVIVTVN SWSNSQSYVG VLQSNVELLR GILPAVAHHC PKCLLLVASQ PVEIMTYATW
     KLSGFPHNRV LGIGCNLDSG RFRHVIEKLA DSEEGAQGAW IIGEQSDNKV AVWGAPDSSA
     NRQTPCKLYP KIFQEQLTSR ALEILKGKGQ RSWSVGLSVA DITDTLVQNK GKVHSVSALC
     KGQFGVQEEV FLSIPCVLGS AGVTGAVQTL QDEAQIWETL QRSAAAIESV QQQLRL