UEX_DROME
ID UEX_DROME Reviewed; 834 AA.
AC A0A0B7P9G0;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-2015, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Unextended protein {ECO:0000312|FlyBase:FBgn0262124};
DE AltName: Full=Putative metal transporter uex {ECO:0000305};
DE Flags: Precursor;
GN Name=uex {ECO:0000312|FlyBase:FBgn0262124};
GN Synonyms=41Ad {ECO:0000312|FlyBase:FBgn0262124},
GN GroupIII {ECO:0000312|FlyBase:FBgn0262124},
GN l(2)41Ad {ECO:0000312|FlyBase:FBgn0262124};
GN ORFNames=CG42595 {ECO:0000312|FlyBase:FBgn0262124};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP FUNCTION, INTERACTION WITH PRL-1, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=31404830; DOI=10.1016/j.isci.2019.07.026;
RA Guo P., Xu X., Wang F., Yuan X., Tu Y., Zhang B., Zheng H., Yu D., Ge W.,
RA Gong Z., Yang X., Xi Y.;
RT "A Novel Neuroprotective Role of Phosphatase of Regenerating Liver-1
RT against CO2 Stimulation in Drosophila.";
RL IScience 19:291-302(2019).
CC -!- FUNCTION: Probable metal transporter (By similarity). Acts downstream
CC of PRL-1 and protects the nervous system against olfactory carbon
CC dioxide stimulation (PubMed:31404830). {ECO:0000250|UniProtKB:Q3TWN3,
CC ECO:0000269|PubMed:31404830}.
CC -!- SUBUNIT: Interacts with PRL-1, possibly at the plasma membrane.
CC {ECO:0000269|PubMed:31404830}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:31404830};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in the nervous system
CC results in a held-up wing phenotype after eclosion and upon carbon
CC dioxide stimulation. {ECO:0000269|PubMed:31404830}.
CC -!- SIMILARITY: Belongs to the ACDP family. {ECO:0000305}.
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DR EMBL; AE013599; AIG63332.1; -; Genomic_DNA.
DR EMBL; AE013599; EDP28148.2; -; Genomic_DNA.
DR EMBL; AE013599; EDP28149.2; -; Genomic_DNA.
DR EMBL; AE013599; ELP57407.1; -; Genomic_DNA.
DR RefSeq; NP_001104390.2; NM_001110920.4.
DR RefSeq; NP_001104391.2; NM_001110921.4.
DR RefSeq; NP_001263162.1; NM_001276233.2.
DR RefSeq; NP_001288144.1; NM_001301215.1.
DR AlphaFoldDB; A0A0B7P9G0; -.
DR SMR; A0A0B7P9G0; -.
DR STRING; 7227.FBpp0291436; -.
DR GlyGen; A0A0B7P9G0; 7 sites.
DR PaxDb; A0A0B7P9G0; -.
DR EnsemblMetazoa; FBtr0302226; FBpp0291436; FBgn0262124.
DR EnsemblMetazoa; FBtr0302227; FBpp0291437; FBgn0262124.
DR EnsemblMetazoa; FBtr0334938; FBpp0306957; FBgn0262124.
DR EnsemblMetazoa; FBtr0346683; FBpp0312292; FBgn0262124.
DR GeneID; 5740320; -.
DR KEGG; dme:Dmel_CG42595; -.
DR CTD; 5740320; -.
DR FlyBase; FBgn0262124; uex.
DR VEuPathDB; VectorBase:FBgn0262124; -.
DR eggNOG; KOG2118; Eukaryota.
DR GeneTree; ENSGT00940000169533; -.
DR OMA; DFTIFAE; -.
DR OrthoDB; 1446644at2759; -.
DR PhylomeDB; A0A0B7P9G0; -.
DR BioGRID-ORCS; 5740320; 0 hits in 3 CRISPR screens.
DR ChiTaRS; uex; fly.
DR GenomeRNAi; 5740320; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0262124; Expressed in midgut and 12 other tissues.
DR ExpressionAtlas; A0A0B7P9G0; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006873; P:cellular ion homeostasis; ISS:FlyBase.
DR GO; GO:0071244; P:cellular response to carbon dioxide; IGI:UniProtKB.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0010960; P:magnesium ion homeostasis; IEA:InterPro.
DR CDD; cd04590; CBS_pair_CorC_HlyC_assoc; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR045095; ACDP.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR002550; CNNM.
DR InterPro; IPR044751; Ion_transp-like_CBS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR PANTHER; PTHR12064; PTHR12064; 1.
DR Pfam; PF01595; DUF21; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR PROSITE; PS51371; CBS; 2.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS51846; CNNM; 1.
PE 1: Evidence at protein level;
KW CBS domain; Cell membrane; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..834
FT /note="Unextended protein"
FT /evidence="ECO:0000255"
FT /id="PRO_5015035038"
FT TOPO_DOM 21..182
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..267
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..298
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320..834
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT DOMAIN 182..361
FT /note="CNNM transmembrane"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01193"
FT DOMAIN 380..441
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 448..515
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT REGION 739..765
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 807..834
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 604..656
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00060"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 522
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 743
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 751
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 790
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 834 AA; 93283 MW; 6EAFD9A83D4E3E41 CRC64;
MNTYFISFIT IIIFANGING TSVDTSNKLL LQKANDFNLS QNLSSSRTRR TIANSFRIVG
IRLEDETVET KNGIPTVLVD KEQQFRVFGS GLEENTAITF TNEKNDYGGP CLKPATDLFT
PIEVSSNGFS ALYSVKFPSF INEFFICAKT AEKTTNHSKA ATTTPLEHQG NSDFLKIKTF
EPLIPVWLAI IIIVTCLGFS ALFSGLNLGL MSMDRTELKI LRNTGTEKEK KYASKIAPVR
DQGNYLLCSI LLGNVLVNST FTILLDGLTS GLFAVIFSTL AIVLFGEITP QAVCSRHGLA
IGAKTILVTK TVMAITAPLS YPVSRILDKL LGEEIGNVYN RERLKELVRV TNDVNDLDKN
EVNIISGALE LRKKTVADVM THINDAFMLS LDALLDFETV SEIMNSGYSR IPVYDGDRKN
IVTLLYIKDL AFVDTDDNTP LKTLCEFYQN PVHFVFEDYT LDIMFNQFKE GTIGHIAFVH
RVNNEGDGDP FYETVGLVTL EDVIEELIQA EIVDETDVFV DNRTKTRRNR YKKADFSAFA
ERREVQTVRI SPQLTLATFQ YLSTAVDAFK KDVISELILR RLLNQDVFHN IKTKGKSKDD
PSLYIFTQGK AVDFFVLILE GRVEVTIGKE ALMFESGPFT YFGTQALVPN VVIDSPTQMG
SLQSLNMDSK IRQSFVPDYS VRAISDVIYI TIKRVLYLTA KKATLLEKSR KSGTFSSETF
DDEVERLLHS ITENEKPSCF AQNQSTRRLS NRSINSSPTN MNRSPDFVYN SVDEAIQDDT
KLKNIKHADN VTTSISLVAA ELEDLHSGEQ DTTAASMPLL PKLDDKFESK QSKP
