UFAA1_MYCTU
ID UFAA1_MYCTU Reviewed; 427 AA.
AC O53732; F2GMG5; I6Y3T0; L0T6H6;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 3.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Tuberculostearic acid methyltransferase UfaA1 {ECO:0000305};
DE Short=TSA methyltransferase {ECO:0000303|PubMed:24033595};
DE EC=2.1.1.- {ECO:0000269|PubMed:24033595};
GN Name=ufaA1 {ECO:0000303|PubMed:24033595};
GN OrderedLocusNames=Rv0447c {ECO:0000312|EMBL:CCP43178.1},
GN RVBD_0447c {ECO:0000312|EMBL:AFN48302.1};
GN ORFNames=LH57_02385 {ECO:0000312|EMBL:AIR13168.1},
GN P425_00460 {ECO:0000312|EMBL:KBJ40223.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genome Sequencing Platform;
RA Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND PATHWAY.
RC STRAIN=H37Rv;
RX PubMed=24033595; DOI=10.1002/bab.1112;
RA Meena L.S., Kolattukudy P.E.;
RT "Expression and characterization of Rv0447c product, potentially the
RT methyltransferase involved in tuberculostearic acid biosynthesis in
RT Mycobacterium tuberculosis.";
RL Biotechnol. Appl. Biochem. 60:412-416(2013).
CC -!- FUNCTION: Involved in the biosynthesis of the tuberculostearic acid
CC (10-methylstearic-acid or TSA), a constituent lipid of the
CC mycobacterial cell wall. Catalyzes the transfer of the methyl group
CC from S-adenosyl-L-methionine (SAM) to the double bond of oleic acid in
CC phosphatidylethanolamine or phosphatidylcholine to produce TSA.
CC {ECO:0000269|PubMed:24033595}.
CC -!- ACTIVITY REGULATION: Inhibited by S-adenosyl-L-homocysteine.
CC {ECO:0000269|PubMed:24033595}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=14.2 uM for dioleyl phosphatidylethanolamine
CC {ECO:0000269|PubMed:24033595};
CC KM=12.5 uM for SAM {ECO:0000269|PubMed:24033595};
CC KM=83.3 uM for NADPH {ECO:0000269|PubMed:24033595};
CC pH dependence:
CC Optimum pH is 7-8. {ECO:0000269|PubMed:24033595};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000269|PubMed:24033595}.
CC -!- SIMILARITY: Belongs to the CFA/CMAS family. {ECO:0000305}.
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DR EMBL; AL123456; CCP43178.1; -; Genomic_DNA.
DR EMBL; CP003248; AFN48302.1; -; Genomic_DNA.
DR EMBL; JLDD01000005; KBJ40223.1; -; Genomic_DNA.
DR EMBL; CP009480; AIR13168.1; -; Genomic_DNA.
DR RefSeq; NP_214961.1; NC_000962.3.
DR RefSeq; WP_003402250.1; NZ_NVQJ01000002.1.
DR AlphaFoldDB; O53732; -.
DR SMR; O53732; -.
DR STRING; 83332.Rv0447c; -.
DR PaxDb; O53732; -.
DR PRIDE; O53732; -.
DR DNASU; 886330; -.
DR GeneID; 886330; -.
DR KEGG; mtu:Rv0447c; -.
DR KEGG; mtv:RVBD_0447c; -.
DR PATRIC; fig|83332.111.peg.488; -.
DR TubercuList; Rv0447c; -.
DR eggNOG; COG2230; Bacteria.
DR HOGENOM; CLU_026434_0_2_11; -.
DR OMA; LDPTMMY; -.
DR PhylomeDB; O53732; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR003333; Mycolic_cyclopropane_synthase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PIRSF; PIRSF003085; CMAS; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Lipid biosynthesis; Lipid metabolism;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..427
FT /note="Tuberculostearic acid methyltransferase UfaA1"
FT /id="PRO_0000432977"
SQ SEQUENCE 427 AA; 47767 MW; 574EC1752799E511 CRC64;
MTVETSQTPS AAIDSDRWPA VAKVPRGPLA AASAAIANRL LRRTATHLPL RLVYSDGTAT
GAADPRAPSL FIHRPDALAR RIGRHGLIGF GESYMAGEWS SKELTRVLTV LAGSVDELVP
RSLHWLRPIT PTFRPSWPDH SRDQARRNIA VHYDLSNDLF AAFLDETMTY SCAMFTDLLA
QPTPAWTELA AAQRRKIDRL LDVAGVQQGS HVLEIGTGWG ELCIRAAARG AHIRSVTLSV
EQQRLARQRV AAAGFGHRVE IDLCDYRDVD GQYDSVVSVE MIEAVGYRSW PRYFAALEQL
VRPGGPVAIQ AITMPHHRML ATRHTQTWIQ KYIFPGGLLP STQAIIDITG QHTGLRIVDA
ASLRPHYAET LRLWRERFMQ RRDGLAHLGF DEVFARMWEL YLAYSEAGFR SGYLDVYQWT
LIREGPP
