UFC1_DROME
ID UFC1_DROME Reviewed; 164 AA.
AC Q7K1Z5; A0A1B2AJX6;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Ubiquitin-fold modifier-conjugating enzyme 1;
DE AltName: Full=Ufm1-conjugating enzyme 1;
GN Name=Ufc1 {ECO:0000312|FlyBase:FBgn0034061};
GN ORFNames=CG8386 {ECO:0000312|FlyBase:FBgn0034061};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ANY27461.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ANY27461.1};
RA Florea S., Webb J.S., Jaromczyk J., Schardl C.L.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26872069; DOI=10.1371/journal.pone.0149039;
RA Duan R., Shi Y., Yu L., Zhang G., Li J., Lin Y., Guo J., Wang J., Shen L.,
RA Jiang H., Wang G., Tang B.;
RT "UBA5 mutations cause a new form of autosomal recessive cerebellar
RT ataxia.";
RL PLoS ONE 11:E0149039-E0149039(2016).
CC -!- FUNCTION: E2-like enzyme which forms an intermediate with UFM1 via a
CC thioester linkage (By similarity). As part of Ufm1 cascade, might play
CC a role in the development of the neuromuscular junctions
CC (PubMed:26872069). {ECO:0000250|UniProtKB:Q9Y3C8,
CC ECO:0000269|PubMed:26872069}.
CC -!- SUBUNIT: Interacts with Uba5 (via C-terminus). Interacts with Ufl1.
CC {ECO:0000250|UniProtKB:Q9Y3C8}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown shows aberrant
CC neuromuscular junctions in the larval muscle, and abnormal wings
CC together with locomotive defects in the adult.
CC {ECO:0000269|PubMed:26872069}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UFC1
CC subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAF58080.1; -; Genomic_DNA.
DR EMBL; AY061366; AAL28914.1; -; mRNA.
DR EMBL; KX531651; ANY27461.1; -; mRNA.
DR RefSeq; NP_611074.1; NM_137230.3.
DR AlphaFoldDB; Q7K1Z5; -.
DR SMR; Q7K1Z5; -.
DR BioGRID; 62488; 1.
DR STRING; 7227.FBpp0086402; -.
DR PaxDb; Q7K1Z5; -.
DR PRIDE; Q7K1Z5; -.
DR DNASU; 36762; -.
DR EnsemblMetazoa; FBtr0087264; FBpp0086402; FBgn0034061.
DR GeneID; 36762; -.
DR KEGG; dme:Dmel_CG8386; -.
DR UCSC; CG8386-RA; d. melanogaster.
DR CTD; 51506; -.
DR FlyBase; FBgn0034061; Ufc1.
DR VEuPathDB; VectorBase:FBgn0034061; -.
DR eggNOG; KOG3357; Eukaryota.
DR GeneTree; ENSGT00390000008196; -.
DR HOGENOM; CLU_101170_0_0_1; -.
DR InParanoid; Q7K1Z5; -.
DR OMA; IKYVQNN; -.
DR OrthoDB; 1339876at2759; -.
DR PhylomeDB; Q7K1Z5; -.
DR BioGRID-ORCS; 36762; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36762; -.
DR PRO; PR:Q7K1Z5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034061; Expressed in oviduct (Drosophila) and 40 other tissues.
DR Genevisible; Q7K1Z5; DM.
DR GO; GO:0061657; F:UFM1 conjugating enzyme activity; ISS:FlyBase.
DR GO; GO:0071568; F:UFM1 transferase activity; IBA:GO_Central.
DR GO; GO:1990592; P:protein K69-linked ufmylation; IBA:GO_Central.
DR GO; GO:0071569; P:protein ufmylation; ISS:FlyBase.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR014806; Ufc1.
DR PANTHER; PTHR12921; PTHR12921; 1.
DR Pfam; PF08694; UFC1; 1.
DR PIRSF; PIRSF008716; DUF1782; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..164
FT /note="Ubiquitin-fold modifier-conjugating enzyme 1"
FT /id="PRO_0000391970"
FT ACT_SITE 116
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q9Y3C8"
SQ SEQUENCE 164 AA; 19004 MW; 24D45F4FF25D3EDA CRC64;
MVDDSTRKTL SNIPLLQIRA GPREKDVWVQ RLKEEYQALI KYVENNKQSG SDWFRLESNK
EGTKWFGKCW YMHNLLKYEF DVEFDIPVTY PTTAPEIALP ELDGKTAKMY RGGKICLTDH
FKPLWARNVP KFGIAHAMAL GLAPWLAVEI PDLIEKGIIT YKEK
