UFC1_MOUSE
ID UFC1_MOUSE Reviewed; 167 AA.
AC Q9CR09;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Ubiquitin-fold modifier-conjugating enzyme 1 {ECO:0000305};
DE Short=Ufm1-conjugating enzyme 1 {ECO:0000250|UniProtKB:Q9Y3C8};
GN Name=Ufc1 {ECO:0000312|MGI:MGI:1913405};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: E1-like enzyme which specifically catalyzes the second step
CC in ufmylation. Accepts the ubiquitin-like modifier UFM1 from the E1
CC enzyme UBA5 and forms an intermediate with UFM1 via a thioester
CC linkage. Ufmylation is involved in reticulophagy (also called ER-phagy)
CC induced in response to endoplasmic reticulum stress.
CC {ECO:0000250|UniProtKB:Q9Y3C8}.
CC -!- SUBUNIT: Interacts with UBA5 (via C-terminus). Interacts with UFL1.
CC Interacts with KIRREL3. Interacts with UFM1.
CC {ECO:0000250|UniProtKB:Q9Y3C8}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UFC1
CC subfamily. {ECO:0000305}.
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DR EMBL; AK003070; BAB22546.1; -; mRNA.
DR EMBL; AK003897; BAB23063.1; -; mRNA.
DR EMBL; BC021936; AAH21936.1; -; mRNA.
DR CCDS; CCDS15488.1; -.
DR RefSeq; NP_079664.1; NM_025388.2.
DR AlphaFoldDB; Q9CR09; -.
DR BMRB; Q9CR09; -.
DR SMR; Q9CR09; -.
DR BioGRID; 211256; 10.
DR IntAct; Q9CR09; 2.
DR MINT; Q9CR09; -.
DR STRING; 10090.ENSMUSP00000078914; -.
DR iPTMnet; Q9CR09; -.
DR PhosphoSitePlus; Q9CR09; -.
DR SwissPalm; Q9CR09; -.
DR EPD; Q9CR09; -.
DR jPOST; Q9CR09; -.
DR MaxQB; Q9CR09; -.
DR PaxDb; Q9CR09; -.
DR PeptideAtlas; Q9CR09; -.
DR PRIDE; Q9CR09; -.
DR ProteomicsDB; 298124; -.
DR Antibodypedia; 34296; 221 antibodies from 26 providers.
DR DNASU; 66155; -.
DR Ensembl; ENSMUST00000080001; ENSMUSP00000078914; ENSMUSG00000062963.
DR Ensembl; ENSMUST00000111302; ENSMUSP00000106933; ENSMUSG00000062963.
DR GeneID; 66155; -.
DR KEGG; mmu:66155; -.
DR UCSC; uc007dnw.1; mouse.
DR CTD; 51506; -.
DR MGI; MGI:1913405; Ufc1.
DR VEuPathDB; HostDB:ENSMUSG00000062963; -.
DR eggNOG; KOG3357; Eukaryota.
DR GeneTree; ENSGT00390000008196; -.
DR HOGENOM; CLU_101170_0_0_1; -.
DR InParanoid; Q9CR09; -.
DR OMA; IKYVQNN; -.
DR OrthoDB; 1339876at2759; -.
DR PhylomeDB; Q9CR09; -.
DR TreeFam; TF313587; -.
DR BioGRID-ORCS; 66155; 16 hits in 74 CRISPR screens.
DR ChiTaRS; Ufc1; mouse.
DR PRO; PR:Q9CR09; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9CR09; protein.
DR Bgee; ENSMUSG00000062963; Expressed in floor plate of midbrain and 263 other tissues.
DR ExpressionAtlas; Q9CR09; baseline and differential.
DR Genevisible; Q9CR09; MM.
DR GO; GO:0061657; F:UFM1 conjugating enzyme activity; ISS:UniProtKB.
DR GO; GO:0071568; F:UFM1 transferase activity; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; ISO:MGI.
DR GO; GO:1990592; P:protein K69-linked ufmylation; ISS:UniProtKB.
DR GO; GO:0071569; P:protein ufmylation; ISS:UniProtKB.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; ISS:UniProtKB.
DR GO; GO:0061709; P:reticulophagy; ISS:UniProtKB.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR014806; Ufc1.
DR PANTHER; PTHR12921; PTHR12921; 1.
DR Pfam; PF08694; UFC1; 1.
DR PIRSF; PIRSF008716; DUF1782; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..167
FT /note="Ubiquitin-fold modifier-conjugating enzyme 1"
FT /id="PRO_0000082614"
FT ACT_SITE 116
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q9Y3C8"
SQ SEQUENCE 167 AA; 19481 MW; 359C44F2CE1FE3E0 CRC64;
MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNSD NDWFRLESNK
EGTRWFGKCW YIHDFLKYEF DIEFEIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH
FKPLWARNVP KFGLAHLMAL GLGPWLAVEV PDLIQKGVIQ HKEKCSQ
