UFD3_CAEEL
ID UFD3_CAEEL Reviewed; 860 AA.
AC G5EES6; G5EFK5;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Ubiquitin fusion degradation protein 3 homolog {ECO:0000303|PubMed:25721663, ECO:0000312|WormBase:C05C10.6b};
GN Name=ufd-3 {ECO:0000312|WormBase:C05C10.6b};
GN ORFNames=C05C10.6 {ECO:0000312|WormBase:C05C10.6b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP INTERACTION WITH CDC-48.1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=25721663; DOI=10.1016/j.bbrc.2015.02.088;
RA Murayama Y., Ogura T., Yamanaka K.;
RT "Characterization of C-terminal adaptors, UFD-2 and UFD-3, of CDC-48 on the
RT polyglutamine aggregation in C. elegans.";
RL Biochem. Biophys. Res. Commun. 459:154-160(2015).
CC -!- FUNCTION: Plays a role in protein ubiquitination, sorting and
CC degradation through its association with cdc-48.1 and/or cdc-48.2.
CC {ECO:0000250|UniProtKB:Q9Y263}.
CC -!- SUBUNIT: Interacts with cdc-48.1. {ECO:0000269|PubMed:25721663}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25721663}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:C05C10.6b};
CC IsoId=G5EES6-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:C05C10.6a};
CC IsoId=G5EES6-2; Sequence=VSP_059916;
CC -!- TISSUE SPECIFICITY: Expressed in intestine (at protein level).
CC {ECO:0000269|PubMed:25721663}.
CC -!- DOMAIN: The PUL domain mediates the interaction with cdc-48.1 and/or
CC cdc-48.2 C-terminus. {ECO:0000250|UniProtKB:Q9Y263}.
CC -!- DOMAIN: The PFU domain mediates interaction with ubiquitin.
CC {ECO:0000250|UniProtKB:Q9Y263}.
CC -!- SIMILARITY: Belongs to the WD repeat PLAP family. {ECO:0000305}.
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DR EMBL; BX284602; CAA88206.1; -; Genomic_DNA.
DR EMBL; BX284602; CAC42247.1; -; Genomic_DNA.
DR PIR; T18946; T18946.
DR RefSeq; NP_496146.1; NM_063745.1. [G5EES6-1]
DR RefSeq; NP_496147.1; NM_063746.3. [G5EES6-2]
DR AlphaFoldDB; G5EES6; -.
DR SMR; G5EES6; -.
DR STRING; 6239.C05C10.6b; -.
DR EPD; G5EES6; -.
DR PaxDb; G5EES6; -.
DR PeptideAtlas; G5EES6; -.
DR EnsemblMetazoa; C05C10.6a.1; C05C10.6a.1; WBGene00007333. [G5EES6-2]
DR EnsemblMetazoa; C05C10.6b.1; C05C10.6b.1; WBGene00007333. [G5EES6-1]
DR GeneID; 3565222; -.
DR KEGG; cel:CELE_C05C10.6; -.
DR CTD; 3565222; -.
DR WormBase; C05C10.6a; CE02113; WBGene00007333; ufd-3. [G5EES6-2]
DR WormBase; C05C10.6b; CE27796; WBGene00007333; ufd-3. [G5EES6-1]
DR eggNOG; KOG0301; Eukaryota.
DR HOGENOM; CLU_011791_2_0_1; -.
DR InParanoid; G5EES6; -.
DR OMA; HNVCALD; -.
DR OrthoDB; 1274776at2759; -.
DR PhylomeDB; G5EES6; -.
DR Reactome; R-CEL-8951664; Neddylation.
DR Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:G5EES6; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00007333; Expressed in germ line (C elegans) and 4 other tissues.
DR ExpressionAtlas; G5EES6; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0010992; P:ubiquitin recycling; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.10.20.870; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR015155; PFU.
DR InterPro; IPR038122; PFU_sf.
DR InterPro; IPR013535; PUL_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF09070; PFU; 2.
DR Pfam; PF08324; PUL; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51394; PFU; 1.
DR PROSITE; PS51396; PUL; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..860
FT /note="Ubiquitin fusion degradation protein 3 homolog"
FT /id="PRO_0000445616"
FT REPEAT 27..65
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 71..112
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 115..154
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 163..203
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 204..242
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 244..283
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT DOMAIN 397..497
FT /note="PFU"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00727"
FT DOMAIN 586..856
FT /note="PUL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00729"
FT REGION 494..585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 532..547
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 156..157
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_059916"
SQ SEQUENCE 860 AA; 93915 MW; 9C9A32EB075BFE17 CRC64;
MADDGLGDVP METESMPQYT ISHVIEAHKS DTKALAVTQG GCLISGGRDE TVKFWAKKGK
QYTKTHAFEQ PKGITVNSIA YAELADGWRL FVGRRDGTIA VFGPSQAEPY AIFNEHKQNV
CCLHINEKAT HMLSGSWDSN VIIWPITELN SSSFTFQTIV CPGHTLSVWA LASFPDLPNT
YLSASADKTI RMWFGDTTLS VFKGHTDVVR ALVVLSSSHF LSAGNDGHII HWDVASASIL
RKFATQAHEF IYSMTLSDSH ILTTGEDGTL EFWAIDGGKD GNLAIVSEAV IQLPTTNTWD
AKVLLNSDIA VAGSDGRIYI MTTDKNRKAD DDILDAFDAE VVAKLTAKTE RMKQEEHETV
TIKVDIDDRP TQLNLKYKKG TDPGLCAQEF LSENNLPIHY LEEITRFIKD RIPEARAFDL
KSGKKVIVDG KEYDYALGVN FGKGEPDKQM PFNVNESPQF AAQRFVERHQ LPVSVIPSLA
GMISQEMDKL SKGAASAQSG YEDPFTGPGR YVPQGSSNSG GHGADPFTGS GRYVPQASNS
SGFDTGFSGD PLTGDGGYRA SAPENTGSHA VPLSSLPQNK KKPRGPLVPV PDFYIIGLAG
KGEKAIAKLK ELNEKQDAFQ LNPDQINGLE ELFVLPTSSN YSSEVTQSAF EMSLQWPVEH
LTPVLDFLRI ALTHHSLNSY FCDRERGQEL VGRLIAILVS DPADVALKVL VCRCIANAFS
HPVGRNLFAS TELSTLAPLV VRQVLNEKTV LQMSAATALA NWSLALLQQS EQCEQLGPKE
DLLRAILNGI ESVDSFGYLG EDAIIRLLQA LVTVMWGDAS VIRLAKNRNI AQIAARLKDA
VSNDSGKNIA RDIVEMTYAV
