ID   UFE1_YEAST              Reviewed;         346 AA.
AC   P41834; D6W2D8;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 156.
DE   RecName: Full=Syntaxin UFE1;
GN   Name=UFE1; OrderedLocusNames=YOR075W; ORFNames=YOR29-26;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 38626 / AH22 / NRRL Y-12843;
RX   PubMed=8929391; DOI=10.1007/s002940050148;
RA   Downing T.A., Storms R.K.;
RT   "Molecular analysis of UFE1, a Saccharomyces cerevisiae gene essential for
RT   spore formation and vegetative growth.";
RL   Curr. Genet. 30:396-403(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9214619; DOI=10.1093/emboj/16.11.3017;
RA   Lewis M.J., Rayner J.C., Pelham H.R.B.;
RT   "A novel SNARE complex implicated in vesicle fusion with the endoplasmic
RT   reticulum.";
RL   EMBO J. 16:3017-3024(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9133743;
RX   DOI=10.1002/(sici)1097-0061(19970330)13:4<379::aid-yea85>3.0.co;2-g;
RA   Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.;
RT   "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the
RT   presence of two tRNAs and 24 new open reading frames.";
RL   Yeast 13:379-390(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA   Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA   Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA   Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA   Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA   Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA   Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA   Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA   Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA   Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA   Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA   Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA   Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
CC   -!- FUNCTION: Syntaxin required for targeting and fusion of Golgi-derived
CC       retrograde transport vesicles with the ER.
CC   -!- SUBUNIT: Component of a SNARE complex consisting of UFE1, USE1, SEC20
CC       and SEC22 or YKT6.
CC   -!- INTERACTION:
CC       P41834; P28791: SEC20; NbExp=5; IntAct=EBI-20016, EBI-16572;
CC       P41834; P22214: SEC22; NbExp=3; IntAct=EBI-20016, EBI-16577;
CC       P41834; P22213: SLY1; NbExp=4; IntAct=EBI-20016, EBI-17387;
CC       P41834; P33891: TIP20; NbExp=3; IntAct=EBI-20016, EBI-19396;
CC       P41834; P53146: USE1; NbExp=2; IntAct=EBI-20016, EBI-23881;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type
CC       IV membrane protein.
CC   -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}.
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DR   EMBL; L15081; AAC13730.1; -; Genomic_DNA.
DR   EMBL; U53416; AAB50196.1; -; Genomic_DNA.
DR   EMBL; Z74983; CAA99268.1; -; Genomic_DNA.
DR   EMBL; Z70678; CAA94560.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA10854.1; -; Genomic_DNA.
DR   PIR; S66958; S66958.
DR   RefSeq; NP_014718.1; NM_001183494.1.
DR   AlphaFoldDB; P41834; -.
DR   SMR; P41834; -.
DR   BioGRID; 34474; 160.
DR   ComplexPortal; CPX-5304; Endoplasmic reticulum snare complex UFE1-USE1-SEC20-SEC22.
DR   DIP; DIP-2942N; -.
DR   IntAct; P41834; 12.
DR   MINT; P41834; -.
DR   STRING; 4932.YOR075W; -.
DR   MoonDB; P41834; Predicted.
DR   iPTMnet; P41834; -.
DR   MaxQB; P41834; -.
DR   PaxDb; P41834; -.
DR   PRIDE; P41834; -.
DR   EnsemblFungi; YOR075W_mRNA; YOR075W; YOR075W.
DR   GeneID; 854242; -.
DR   KEGG; sce:YOR075W; -.
DR   SGD; S000005601; UFE1.
DR   VEuPathDB; FungiDB:YOR075W; -.
DR   eggNOG; KOG3894; Eukaryota.
DR   GeneTree; ENSGT00390000014853; -.
DR   HOGENOM; CLU_069210_1_0_1; -.
DR   InParanoid; P41834; -.
DR   OMA; RTAKMTT; -.
DR   BioCyc; YEAST:G3O-33612-MON; -.
DR   Reactome; R-SCE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR   PRO; PR:P41834; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   RNAct; P41834; protein.
DR   GO; GO:0098554; C:cytoplasmic side of endoplasmic reticulum membrane; IC:ComplexPortal.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR   GO; GO:0012508; C:Golgi to ER transport vesicle membrane; IC:ComplexPortal.
DR   GO; GO:0016021; C:integral component of membrane; ISM:SGD.
DR   GO; GO:0031201; C:SNARE complex; IDA:SGD.
DR   GO; GO:0005484; F:SNAP receptor activity; ISA:SGD.
DR   GO; GO:0016320; P:endoplasmic reticulum membrane fusion; IMP:SGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IMP:SGD.
DR   GO; GO:0048279; P:vesicle fusion with endoplasmic reticulum; IC:SGD.
DR   InterPro; IPR019529; Syntaxin-18_N.
DR   InterPro; IPR000727; T_SNARE_dom.
DR   Pfam; PF10496; Syntaxin-18_N; 1.
DR   SMART; SM00397; t_SNARE; 1.
DR   PROSITE; PS50192; T_SNARE; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Endoplasmic reticulum; ER-Golgi transport; Membrane;
KW   Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..346
FT                   /note="Syntaxin UFE1"
FT                   /id="PRO_0000210283"
FT   TOPO_DOM        1..324
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..342
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        343..346
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          255..317
FT                   /note="t-SNARE coiled-coil homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
SQ   SEQUENCE   346 AA;  40539 MW;  DDC164793510A897 CRC64;
     MMSDLTPIFR KYVAVIDDAR NEQNGIDDHV ERKQEDFGNS NETCEMFRDS FIKECARLLK
     FLVELNKVIK QIEKNYLDDF NMSDAEKDEF DMECRLQIQQ YFKKFEFLEN YEMERHNLSL
     KRFQSKSHRW SKILSNKNDN TKHVIHPQDI ENGVYEFRLG VLRCLNLWIK YVSSKFTTIQ
     QERLILENKM NFNSTPMPTL SNNADDFSAD AIDISVSQSA PVETVQDEVK HYEETISKLT
     QEQLQVLETE HSELLNQKNE QLKKVETINK TILDIVNIQN ELSNHLTVQS QNINLMLNNQ
     DDIELNIKKG NKELRKAKRA AGRTAKMTTY GAIIMGVFIL FLDYVG