UFL1_DROME
ID UFL1_DROME Reviewed; 782 AA.
AC Q9VI55; Q8INS2;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=E3 UFM1-protein ligase 1 homolog;
DE EC=2.3.2.- {ECO:0000250|UniProtKB:O94874};
DE AltName: Full=E3 UFM1-protein transferase 1 homolog {ECO:0000305};
DE AltName: Full=UFM1 specific ligase 1 {ECO:0000312|FlyBase:FBgn0037467};
GN Name=Ufl1 {ECO:0000312|FlyBase:FBgn0037467};
GN ORFNames=CG1104 {ECO:0000312|FlyBase:FBgn0037467};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING (ISOFORMS A AND B).
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=26872069; DOI=10.1371/journal.pone.0149039;
RA Duan R., Shi Y., Yu L., Zhang G., Li J., Lin Y., Guo J., Wang J., Shen L.,
RA Jiang H., Wang G., Tang B.;
RT "UBA5 mutations cause a new form of autosomal recessive cerebellar
RT ataxia.";
RL PLoS ONE 11:E0149039-E0149039(2016).
CC -!- FUNCTION: E3 UFM1-protein ligase that mediates ufmylation of target
CC proteins. {ECO:0000250|UniProtKB:O94874}.
CC -!- INTERACTION:
CC Q9VI55; Q9VDD1: CG5862; NbExp=4; IntAct=EBI-172922, EBI-148976;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9VI55-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9VI55-2; Sequence=VSP_038767;
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown is lethal.
CC {ECO:0000269|PubMed:26872069}.
CC -!- SIMILARITY: Belongs to the UFL1 family. {ECO:0000305}.
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DR EMBL; AE014297; AAF54073.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13356.1; -; Genomic_DNA.
DR EMBL; AY051887; AAK93311.1; -; mRNA.
DR RefSeq; NP_001246957.1; NM_001260028.2. [Q9VI55-1]
DR RefSeq; NP_649690.1; NM_141433.3. [Q9VI55-1]
DR AlphaFoldDB; Q9VI55; -.
DR BioGRID; 66041; 36.
DR IntAct; Q9VI55; 2.
DR STRING; 7227.FBpp0293859; -.
DR PaxDb; Q9VI55; -.
DR PRIDE; Q9VI55; -.
DR DNASU; 40843; -.
DR EnsemblMetazoa; FBtr0081643; FBpp0081157; FBgn0037467. [Q9VI55-1]
DR EnsemblMetazoa; FBtr0305316; FBpp0293859; FBgn0037467. [Q9VI55-1]
DR GeneID; 40843; -.
DR KEGG; dme:Dmel_CG1104; -.
DR UCSC; CG1104-RA; d. melanogaster. [Q9VI55-1]
DR UCSC; CG1104-RB; d. melanogaster.
DR CTD; 23376; -.
DR FlyBase; FBgn0037467; Ufl1.
DR VEuPathDB; VectorBase:FBgn0037467; -.
DR eggNOG; KOG2235; Eukaryota.
DR GeneTree; ENSGT00390000002112; -.
DR HOGENOM; CLU_012417_1_1_1; -.
DR InParanoid; Q9VI55; -.
DR OMA; NECSATK; -.
DR PhylomeDB; Q9VI55; -.
DR Reactome; R-DME-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q9VI55; -.
DR BioGRID-ORCS; 40843; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40843; -.
DR PRO; PR:Q9VI55; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037467; Expressed in spermathecum and 32 other tissues.
DR ExpressionAtlas; Q9VI55; baseline and differential.
DR Genevisible; Q9VI55; DM.
DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0061666; F:UFM1 ligase activity; ISS:FlyBase.
DR GO; GO:0071568; F:UFM1 transferase activity; IBA:GO_Central.
DR GO; GO:1990592; P:protein K69-linked ufmylation; IBA:GO_Central.
DR GO; GO:0071569; P:protein ufmylation; ISS:FlyBase.
DR GO; GO:0032434; P:regulation of proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IBA:GO_Central.
DR InterPro; IPR018611; Ufl1.
DR PANTHER; PTHR31057; PTHR31057; 1.
DR Pfam; PF09743; E3_UFM1_ligase; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..782
FT /note="E3 UFM1-protein ligase 1 homolog"
FT /id="PRO_0000391882"
FT REGION 404..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..428
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..467
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..43
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_038767"
SQ SEQUENCE 782 AA; 87483 MW; E7D2395E2B566A7F CRC64;
MGSDWDEIKR LAADFQKAQL TSTLQKLSER NCVEIVTLLL EKQMLEVVFT NDGKEYITPD
HLEREIQDEL YVNGGRANLV EVSKTLNVDL SRIELLAERI SAENQSVHLV LGQLIDEDYI
SHIAQEINEK LVQRGEVSIS ELASQFDLPS DFLQHDVVEK HLGKIIKGRQ DASNPRVFFT
QAYIQRCKAK IRGALAAITR PINVAVILQK IGVQEKIFYS LLDEIAPAGQ VTSKQANSQY
VPHIYAKTQA DWVNSFYKQN SFLEYDAIQK LGISDAKSYI RKQFPNEEFL YLKRVALGAR
LVELTVVTAL NECSATKQYL DLTTILPSNL SEEDIEEVFS TIMAQKHSNP SNFVYLDGIV
FSQPYLAQLV QPCQALAESQ AKAAIDGGVY QQYIVEKTLA QKGNVSTQEL EDEGKVDKRD
ERRKKASSGK AGGGAQGRET KTKSTKKHQR GKAAAQFDSD DEDDAQQGSR GGGGASKKAV
KPLELVKTAD IVKLITASLE EEGLEHLSKS IASLYTNQFN QTALARAQEL FEATPQTNRR
QTHAAIQDRI NTLLIDIRLY EKGLKLFPQD TQTQLVKYLL KSLGNEICNE LSLYVASECN
LTVKNTNLNV DQRNKLAQEC EAQYRAALLE QNKALNKSID DFELATETVL KTCSMIIKKV
DKKKDRLLIA DHKKKLQKQL LECNEPALLL HLAALILFTT ITGSILHASG KFVSAILQHI
RGSLNEDQNA LLLRYHDLVL QVLQAIPDSN ESKLANEHLQ TMQNQVVELA QNFSRASISK
AD
