ID   UFOG_GENTR              Reviewed;         453 AA.
AC   Q96493;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Anthocyanidin 3-O-glucosyltransferase;
DE            EC=2.4.1.115;
DE   AltName: Full=Flavonol 3-O-glucosyltransferase;
DE   AltName: Full=UDP-glucose flavonoid 3-O-glucosyltransferase;
OS   Gentiana triflora (Clustered gentian).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Gentianaceae; Gentianeae; Gentianinae;
OC   Gentiana.
OX   NCBI_TaxID=55190;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   TISSUE=Petal;
RX   PubMed=8819318; DOI=10.1093/oxfordjournals.pcp.a029004;
RA   Tanaka Y., Yonekura K., Fukuchi-Mizutani M., Fukui Y., Fujiwara H.,
RA   Ashikari T., Kusumi T.;
RT   "Molecular and biochemical characterization of three anthocyanin synthetic
RT   enzymes from Gentiana triflora.";
RL   Plant Cell Physiol. 37:711-716(1996).
CC   -!- FUNCTION: In the presence of other necessary color factors, this
CC       glycosylation reaction allows the accumulation of anthocyanin pigments
CC       (PubMed:8819318). Anthocyanidins are the preferred substrates, while
CC       flavonols are only a minor substrate in vitro (PubMed:8819318).
CC       {ECO:0000269|PubMed:8819318}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an anthocyanidin + H(+) + UDP-alpha-D-glucose = an
CC         anthocyanidin 3-O-beta-D-glucoside + UDP; Xref=Rhea:RHEA:20093,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16307, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885, ChEBI:CHEBI:143576; EC=2.4.1.115;
CC         Evidence={ECO:0000269|PubMed:8819318};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20094;
CC         Evidence={ECO:0000269|PubMed:8819318};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=delphinidin + UDP-alpha-D-glucose = delphinidin 3-O-beta-D-
CC         glucoside + UDP; Xref=Rhea:RHEA:61500, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885, ChEBI:CHEBI:144775, ChEBI:CHEBI:144776;
CC         EC=2.4.1.115; Evidence={ECO:0000269|PubMed:8819318};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61501;
CC         Evidence={ECO:0000269|PubMed:8819318};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pelargonidin + UDP-alpha-D-glucose = pelargonidin 3-O-beta-D-
CC         glucoside + UDP; Xref=Rhea:RHEA:61504, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885, ChEBI:CHEBI:144777, ChEBI:CHEBI:144778;
CC         EC=2.4.1.115; Evidence={ECO:0000269|PubMed:8819318};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61505;
CC         Evidence={ECO:0000269|PubMed:8819318};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyanidin + UDP-alpha-D-glucose = cyanidin 3-O-beta-D-glucoside
CC         + H(+) + UDP; Xref=Rhea:RHEA:60100, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:71682,
CC         ChEBI:CHEBI:77857; EC=2.4.1.115;
CC         Evidence={ECO:0000269|PubMed:8819318};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60101;
CC         Evidence={ECO:0000269|PubMed:8819318};
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; D85186; BAA12737.1; -; mRNA.
DR   AlphaFoldDB; Q96493; -.
DR   SMR; Q96493; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   UniPathway; UPA00009; -.
DR   GO; GO:0047213; F:anthocyanidin 3-O-glucosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..453
FT                   /note="Anthocyanidin 3-O-glucosyltransferase"
FT                   /id="PRO_0000074141"
SQ   SEQUENCE   453 AA;  50009 MW;  BF738B0A2DA76C05 CRC64;
     MSPVSHVAVL AFPFGTHAAP LLTLVNRLAA SAPDIIFSFF STSSSITTIF SPTNLISIGS
     NIKPYAVWDG SPEGFVFSGN PREPIEYFLN AAPDNFDKAM KKAVEDTGVN ISCLLTDAFL
     WFAADFSEKI GVPWIPVWTA ASCSLCLHVY TDEIRSRFAE FDIAEKAEKT IDFIPGLSAI
     SFSDLPEELI MEDSQSIFAL TLHNMGLKLH KATAVAVNSF EEIDPIITNH LRSTNQLNIL
     NIGPLQTLSS SIPPEDNECL KWLQTQKESS VVYLSFGTVI NPPPNEMAAL ASTLESRKIP
     FLWSLRDEAR KHLPENFIDR TSTFGKIVSW APQLHVLENP AIGVFVTHCG WNSTLESIFC
     RVPVIGRPFF GDQKVNARMV EDVWKIGVGV KGGVFTEDET TRVLELVLFS DKGKEMRQNV
     GRLKEKAKDA VKANGSSTRN FESLLAAFNK LDS