ID   UFOG_PETHY              Reviewed;         473 AA.
AC   Q43716; Q40895;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Anthocyanidin-3-O-glucoside rhamnosyltransferase {ECO:0000303|PubMed:8130800};
DE            EC=2.4.1.- {ECO:0000305};
DE   AltName: Full=Anthocyanin rhamnosyl transferase {ECO:0000303|PubMed:8130799};
GN   Name=RT {ECO:0000303|PubMed:8130799}; Synonyms=UGT72 {ECO:0000305};
OS   Petunia hybrida (Petunia).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX   NCBI_TaxID=4102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC   STRAIN=cv. Violet 26; TISSUE=Leaf;
RX   PubMed=8130799; DOI=10.1046/j.1365-313x.1994.5010069.x;
RA   Kroon J., Souer E., de Graaff A., Xue Y., Mol J., Koes R.;
RT   "Cloning and structural analysis of the anthocyanin pigmentation locus Rt
RT   of Petunia hybrida: characterization of insertion sequences in two mutant
RT   alleles.";
RL   Plant J. 5:69-80(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 3-473, FUNCTION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Old Glory Blue; TISSUE=Petal;
RX   PubMed=8130800; DOI=10.1046/j.1365-313x.1994.5010081.x;
RA   Brugliera F., Holton T.A., Stevenson T.W., Farcy E., Lu C.Y., Cornish E.C.;
RT   "Isolation and characterization of a cDNA clone corresponding to the Rt
RT   locus of Petunia hybrida.";
RL   Plant J. 5:81-92(1994).
CC   -!- FUNCTION: Controls the rhamnosylation of reddish anthocyanidin-3-O-
CC       glucosides, which is the first step in a series of modifications that
CC       finally yield magenta or blue/purple coloured anthocyanins
CC       (PubMed:8130799, PubMed:8130800). Controls the conversion of
CC       anthocyanidin-3-O-glucosides to anthocyanidin-3-O-rutinosides
CC       (PubMed:8130800). {ECO:0000269|PubMed:8130799,
CC       ECO:0000269|PubMed:8130800}.
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis. {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in petals, styles and anthers.
CC       {ECO:0000269|PubMed:8130800}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at high levels in petals from flowers at
CC       an early stage of development, and expression levels decline as the
CC       flower matures. {ECO:0000269|PubMed:8130800}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X71060; CAA50377.1; -; Genomic_DNA.
DR   EMBL; X71059; CAA50376.1; -; mRNA.
DR   EMBL; Z25802; CAA81057.1; -; mRNA.
DR   PIR; S36655; S36655.
DR   PIR; S60290; S60290.
DR   AlphaFoldDB; Q43716; -.
DR   SMR; Q43716; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   UniPathway; UPA00009; -.
DR   GO; GO:0051566; F:anthocyanidin-3-glucoside rhamnosyltransferase activity; IMP:UniProtKB.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IMP:UniProtKB.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..473
FT                   /note="Anthocyanidin-3-O-glucoside rhamnosyltransferase"
FT                   /id="PRO_0000074149"
FT   CONFLICT        246..255
FT                   /note="LIGPVVPDPP -> SNRTRSSGPA (in Ref. 1; CAA50377)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        348
FT                   /note="N -> H (in Ref. 2; CAA81057)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        431
FT                   /note="D -> E (in Ref. 2; CAA81057)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   473 AA;  52406 MW;  A310E4BFBC9EB02A CRC64;
     MENEMKHSND ALHVVMFPFF AFGHISPFVQ LANKLSSYGV KVSFFTASGN ASRVKSMLNS
     APTTHIVPLT LPHVEGLPPG AESTAELTPA SAELLKVALD LMQPQIKTLL SHLKPHFVLF
     DFAQEWLPKM ANGLGIKTVY YSVVVALSTA FLTCPARVLE PKKYPSLEDM KKPPLGFPQT
     SVTSVRTFEA RDFLYVFKSF HNGPTLYDRI QSGLRGCSAI LAKTCSQMEG PYIKYVEAQF
     NKPVFLIGPV VPDPPSGKLE EKWATWLNKF EGGTVIYCSF GSETFLTDDQ VKELALGLEQ
     TGLPFFLVLN FPANVDVSAE LNRALPEGFL ERVKDKGIIH SGWVQQQNIL AHSSVGCYVC
     HAGFSSVIEA LVNDCQVVML PQKGDQILNA KLVSGDMEAG VEINRRDEDG YFGKEDIKEA
     VEKVMVDVEK DPGKLIRENQ KKWKEFLLNK DIQSKYIGNL VNEMTAMAKV STT