UFO_ARATH
ID UFO_ARATH Reviewed; 442 AA.
AC Q39090; Q4PT17; Q9FYI4;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 09-MAY-2003, sequence version 2.
DT 25-MAY-2022, entry version 153.
DE RecName: Full=Protein UNUSUAL FLORAL ORGANS;
DE AltName: Full=F-box only protein 1;
DE Short=AtFBX1;
GN Name=UFO; Synonyms=FBX1; OrderedLocusNames=At1g30950; ORFNames=F17F8.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=8589630; DOI=10.2307/3870138;
RA Ingram G.C., Goodrich J., Wilkinson M.D., Simon R., Haughn G.W., Coen E.S.;
RT "Parallels between UNUSUAL FLORAL ORGANS and FIMBRIATA, genes controlling
RT flower development in Arabidopsis and Antirrhinum.";
RL Plant Cell 7:1501-1510(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=8565821; DOI=10.1242/dev.122.1.11;
RA Krizek B.A., Meyerowitz E.M.;
RT "The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to
RT provide the B class organ identity function.";
RL Development 122:11-22(1996).
RN [6]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=9016705; DOI=10.1016/s0960-9822(06)00053-4;
RA Lee I., Wolfe D.S., Nilsson O., Weigel D.;
RT "A LEAFY co-regulator encoded by UNUSUAL FLORAL ORGANS.";
RL Curr. Biol. 7:95-104(1997).
RN [7]
RP FUNCTION.
RX PubMed=10607296; DOI=10.1046/j.1365-313x.1999.00617.x;
RA Samach A., Klenz J.E., Kohalmi S.E., Risseeuw E., Haughn G.W., Crosby W.L.;
RT "The UNUSUAL FLORAL ORGANS gene of Arabidopsis thaliana is an F-box protein
RT required for normal patterning and growth in the floral meristem.";
RL Plant J. 20:433-445(1999).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11077244; DOI=10.1016/s1360-1385(00)01769-6;
RA Xiao W., Jang J.-C.;
RT "F-box proteins in Arabidopsis.";
RL Trends Plant Sci. 5:454-457(2000).
RN [9]
RP SUBUNIT.
RX PubMed=11526079; DOI=10.1242/dev.128.14.2735;
RA Zhao D., Yu Q., Chen M., Ma H.;
RT "The ASK1 gene regulates B function gene expression in cooperation with UFO
RT and LEAFY in Arabidopsis.";
RL Development 128:2735-2746(2001).
RN [10]
RP INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2 AND ASK11.
RX PubMed=12169662; DOI=10.1073/pnas.162339999;
RA Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D.;
RT "The F-box subunit of the SCF E3 complex is encoded by a diverse
RT superfamily of genes in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002).
RN [11]
RP INTERACTION WITH SKP1A, AND DOMAIN.
RX PubMed=12826617; DOI=10.1073/pnas.1033043100;
RA Durfee T., Roe J.L., Sessions R.A., Inouye C., Serikawa K., Feldmann K.A.,
RA Weigel D., Zambryski P.C.;
RT "The F-box-containing protein UFO and AGAMOUS participate in antagonistic
RT pathways governing early petal development in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:8571-8576(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins (By similarity). Considered
CC as a meristem identity factor required for normal growth of the young
CC floral meristem. Acts together with LEAFY to positively regulate the B
CC class floral homeotic genes APETALA3 and PISTILLATA. In this way,
CC operates as a region-specific regulator for petal and stamen
CC development. Alternatively, may play a role as a negative regulator of
CC the C class floral homeotic genes. Interacts together with the SKP1-
CC like protein ASK1 to form a ubiquitin E3 ligase complex and could
CC indirectly promote the ubiquitination and degradation of specific
CC proteins controlling the floral primordia development like repressors
CC of B class floral homeotic genes. {ECO:0000250,
CC ECO:0000269|PubMed:10607296, ECO:0000269|PubMed:8565821,
CC ECO:0000269|PubMed:9016705}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a putative SCF (ASK/Cullin/F-box) ubiquitin ligase
CC complex. Interacts with SKP1A/ASK1, SKP1B/ASK2 and ASK11.
CC {ECO:0000269|PubMed:11526079, ECO:0000269|PubMed:12169662,
CC ECO:0000269|PubMed:12826617}.
CC -!- INTERACTION:
CC Q39090; O49484: ASK11; NbExp=3; IntAct=EBI-590758, EBI-401185;
CC Q39090; Q00958: LFY; NbExp=6; IntAct=EBI-590758, EBI-1644366;
CC Q39090; Q39255: SKP1A; NbExp=6; IntAct=EBI-590758, EBI-532357;
CC Q39090; Q9FHW7: SKP1B; NbExp=5; IntAct=EBI-590758, EBI-604076;
CC Q39090; P43291: SRK2A; NbExp=3; IntAct=EBI-590758, EBI-401164;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Detected early throughout the shoot apical
CC meristem, but not in the emerging leaf primordia. Restricted later to
CC the junction between sepal and petal primordia.
CC {ECO:0000269|PubMed:9016705}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Mutations in the UFO gene result in the formation of
CC unusual floral organs.
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DR EMBL; X89224; CAA61508.1; -; Genomic_DNA.
DR EMBL; AC000107; AAF98199.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31296.1; -; Genomic_DNA.
DR EMBL; DQ056469; AAY78626.1; -; mRNA.
DR PIR; S57710; S57710.
DR RefSeq; NP_564368.1; NM_102834.2.
DR AlphaFoldDB; Q39090; -.
DR BioGRID; 25217; 15.
DR IntAct; Q39090; 15.
DR STRING; 3702.AT1G30950.1; -.
DR PaxDb; Q39090; -.
DR PRIDE; Q39090; -.
DR EnsemblPlants; AT1G30950.1; AT1G30950.1; AT1G30950.
DR GeneID; 839982; -.
DR Gramene; AT1G30950.1; AT1G30950.1; AT1G30950.
DR KEGG; ath:AT1G30950; -.
DR Araport; AT1G30950; -.
DR TAIR; locus:2015766; AT1G30950.
DR eggNOG; ENOG502QR12; Eukaryota.
DR HOGENOM; CLU_038778_2_1_1; -.
DR InParanoid; Q39090; -.
DR OMA; ESFHIDA; -.
DR OrthoDB; 1044393at2759; -.
DR PhylomeDB; Q39090; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q39090; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q39090; baseline and differential.
DR Genevisible; Q39090; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IMP:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IGI:TAIR.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR Pfam; PF00646; F-box; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; Flowering; Nucleus;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation pathway.
FT CHAIN 1..442
FT /note="Protein UNUSUAL FLORAL ORGANS"
FT /id="PRO_0000119966"
FT DOMAIN 44..90
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 1..85
FT /note="Interaction with SKP1A"
FT /evidence="ECO:0000269|PubMed:12826617"
FT CONFLICT 44
FT /note="G -> C (in Ref. 1; CAA61508)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 442 AA; 49047 MW; 6E5C38B0F92AE8B7 CRC64;
MDSTVFINNP SLTLPFSYTF TSSSNSSTTT STTTDSSSGQ WMDGRIWSKL PPPLLDRVIA
FLPPPAFFRT RCVCKRFYSL LFSNTFLETY LQLLPLRHNC FLFFKHKTLK SYIYKRGGTN
DDDSNKAEGF LFDPNEIRWY RLSFAYIPSG FYPSGSSGGL VSWVSEEAGL KTILLCNPLV
GSVSQLPPIS RPRLFPSIGL SVTPTSIDVT VAGDDLISPY AVKNLSSESF HVDAGGFFSL
WAMTSSLPRL CSLESGKMVY VQGKFYCMNY SPFSVLSYEV TGNRWIKIQA PMRRFLRSPS
LLESKGRLIL VAAVEKSKLN VPKSLRLWSL QQDNATWVEI ERMPQPLYTQ FAAEEGGKGF
ECVGNQEFVM IVLRGTSLQL LFDIVRKSWL WVPPCPYSGS GGGSSGGGSD GEVLQGFAYD
PVLTTPVVSL LDQLTLPFPG VC
