ID   UFSP_CAEBR              Reviewed;         588 AA.
AC   Q61UA0; A8WZT5;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Ufm1-specific protease {ECO:0000250|UniProtKB:Q9NUQ7};
DE            Short=UfSP {ECO:0000250|UniProtKB:Q9NUQ7};
DE            EC=3.4.22.-;
DE   AltName: Full=Odorant response abnormal protein 8;
GN   Name=odr-8 {ECO:0000312|WormBase:CBG05410};
GN   Synonyms=ufsp-2 {ECO:0000312|WormBase:CBG05410};
GN   ORFNames=CBG05410 {ECO:0000312|WormBase:CBG05410};
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Thiol protease which recognizes and hydrolyzes the peptide
CC       bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein
CC       bound to a number of target proteins. Required, with oct-4, for the
CC       localization of a subset of 7 transmembrane domain odorant receptors,
CC       including odr-10, to the cilia of olfactory neurons AWA and AWC.
CC       Operates in aggregation behavior, and responses to oxygen levels.
CC       {ECO:0000250|UniProtKB:Q94218, ECO:0000250|UniProtKB:Q9NUQ7}.
CC   -!- SUBUNIT: Interacts with odr-4. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q94218}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q94218}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q94218}. Cytoplasm, perinuclear region
CC       {ECO:0000250|UniProtKB:Q94218}. Note=Recruited to the endoplasmic
CC       reticulum upon interaction with odr-4. {ECO:0000250|UniProtKB:Q94218}.
CC   -!- SIMILARITY: Belongs to the peptidase C78 family. {ECO:0000305}.
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DR   EMBL; HE601369; CAP25895.1; -; Genomic_DNA.
DR   RefSeq; XP_002633543.1; XM_002633497.1.
DR   AlphaFoldDB; Q61UA0; -.
DR   SMR; Q61UA0; -.
DR   STRING; 6238.CBG05410; -.
DR   MEROPS; C78.A03; -.
DR   EnsemblMetazoa; CBG05410.1; CBG05410.1; WBGene00027865.
DR   GeneID; 8575540; -.
DR   KEGG; cbr:CBG_05410; -.
DR   CTD; 8575540; -.
DR   WormBase; CBG05410; CBP01387; WBGene00027865; Cbr-odr-8.
DR   eggNOG; KOG2433; Eukaryota.
DR   HOGENOM; CLU_033013_0_0_1; -.
DR   InParanoid; Q61UA0; -.
DR   OMA; VFLPAGW; -.
DR   OrthoDB; 390107at2759; -.
DR   Proteomes; UP000008549; Chromosome IV.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0032991; C:protein-containing complex; IEA:EnsemblMetazoa.
DR   GO; GO:0071567; F:deUFMylase activity; IEA:EnsemblMetazoa.
DR   GO; GO:0050921; P:positive regulation of chemotaxis; IEA:EnsemblMetazoa.
DR   GO; GO:0097499; P:protein localization to non-motile cilium; IEA:EnsemblMetazoa.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0045471; P:response to ethanol; IEA:EnsemblMetazoa.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR012462; Peptidase_C78_UfSP1/2.
DR   Pfam; PF07910; Peptidase_C78; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Hydrolase; Membrane; Protease;
KW   Reference proteome; Thiol protease; Ubl conjugation pathway.
FT   CHAIN           1..588
FT                   /note="Ufm1-specific protease"
FT                   /id="PRO_0000280369"
FT   ACT_SITE        420
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        544
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        546
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   588 AA;  66332 MW;  9F948E0D80EA1665 CRC64;
     MSGSQTVSVI GYTKMAPQSP PATVNELWFI DTQAMFQNYA NLRSFSKSNS TETQTTIGGV
     VFGRKARKQV IHVFFAYAED LTESNLQFLE SSLSSDIELV GNVNIDGQST LIGNGTFTLQ
     LSSKMLENKN TSEFLDQNVI FNNDHISMEG ASCVSKVGFE WSLRAGREQE DVKSAAERLS
     MASFRFSYLN AEHELVIREH KPETAKQKYM DKFTKGALPY KDVIEFTAMQ SLTRDTSNDT
     EDQKLVPTVK VTKDNKHFTR LVTIGEVVFP AYFGDSSFDL YKRAREALNR RANNTMMVTV
     NGIRSGRGVT TTTSATYLPP GWVSLLHLQL PSKWTENEKR NYRIRLHKLF NLPSSKPCLR
     LSQSLPLHSE SVRLTNKKLI REPHLSISNY QPAGVVTAVK GPYNYHHYMQ DGIDDSGWGC
     AYRSFQTIWS WFILNGYTDK PVPSHRDIQQ ALVNIGDKEQ KFVGSRQWIG STEISYVLNE
     LLKLECRFIA TNSGAEVVER ARELARHFET SGTPVMIGGN MLAHTILGVD FNEMTGETKF
     LILDPHYTGS EDIKTITSKG WCAWKPASFW SADHFYNMVL AQPPTDSI