ID   UGA3_YEAST              Reviewed;         528 AA.
AC   P26370; D6VRI1;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 164.
DE   RecName: Full=Transcriptional activator protein UGA3;
GN   Name=UGA3; OrderedLocusNames=YDL170W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sigma 1278B;
RX   PubMed=2109179; DOI=10.1007/bf00260493;
RA   Andre B.;
RT   "The UGA3 gene regulating the GABA catabolic pathway in Saccharomyces
RT   cerevisiae codes for a putative zinc-finger protein acting on RNA amount.";
RL   Mol. Gen. Genet. 220:269-276(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 453-528.
RC   STRAIN=ATCC 38626 / AH22 / NRRL Y-12843;
RA   Lindner Z.;
RL   Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: GABA-dependent positive regulation of genes required for
CC       catabolism of GABA (UGA4, UGA1, and UGA2).
CC   -!- SUBUNIT: UGA3 proteins associate in oligomers, at least in the presence
CC       of inducer.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Present with 1200 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; X51664; CAA35976.1; -; Genomic_DNA.
DR   EMBL; Z67750; CAA91576.1; -; Genomic_DNA.
DR   EMBL; Z74218; CAA98744.1; -; Genomic_DNA.
DR   EMBL; X82846; CAA58049.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA11691.1; -; Genomic_DNA.
DR   PIR; S14944; S14944.
DR   RefSeq; NP_010111.1; NM_001180230.1.
DR   AlphaFoldDB; P26370; -.
DR   SMR; P26370; -.
DR   BioGRID; 31895; 82.
DR   STRING; 4932.YDL170W; -.
DR   MaxQB; P26370; -.
DR   PaxDb; P26370; -.
DR   PRIDE; P26370; -.
DR   EnsemblFungi; YDL170W_mRNA; YDL170W; YDL170W.
DR   GeneID; 851384; -.
DR   KEGG; sce:YDL170W; -.
DR   SGD; S000002329; UGA3.
DR   VEuPathDB; FungiDB:YDL170W; -.
DR   eggNOG; ENOG502RIXM; Eukaryota.
DR   HOGENOM; CLU_015493_1_2_1; -.
DR   InParanoid; P26370; -.
DR   OMA; MPGTIDP; -.
DR   BioCyc; YEAST:G3O-29561-MON; -.
DR   ChiTaRS; UGA3; yeast.
DR   PRO; PR:P26370; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; P26370; protein.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:SGD.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR   GO; GO:0001010; F:RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity; IDA:SGD.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0009450; P:gamma-aminobutyric acid catabolic process; IMP:SGD.
DR   GO; GO:0001080; P:nitrogen catabolite activation of transcription from RNA polymerase II promoter; IMP:SGD.
DR   GO; GO:0019740; P:nitrogen utilization; IMP:SGD.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR021858; Fun_TF.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF11951; Fungal_trans_2; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..528
FT                   /note="Transcriptional activator protein UGA3"
FT                   /id="PRO_0000114986"
FT   DNA_BIND        17..44
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   MOTIF           55..62
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         224
FT                   /note="G->R: Constitutive activity; when associated with R-
FT                   451."
FT   MUTAGEN         451
FT                   /note="G->R: Loss of activity. Constitutive activity; when
FT                   associated with R-224."
SQ   SEQUENCE   528 AA;  61224 MW;  DFA04DDE29937942 CRC64;
     MNYGVEKLKL KYSKHGCITC KIRKKRCSED KPVCRDCRRL SFPCIYISES VDKQSLKKIK
     ADIQHQLISK KRKHAPDSAQ KAAVATRTRR VGSDEQDNQV YLSKPLEDCI SQKLDSMGLQ
     LYNYYRSHLA NIISIAPMNQ NYYLNIFLPM AHENDGILFA ILAWSANHLS ISSSNELRKD
     EIFVNLANKY TYMSLSHLKT NEGSSACAKL GFLYSLAQIL ILCGSEICQG DVKFWKILLN
     IGKNLIENHV GKDVSRILTT TTEEPSLEER IIFPNFNSVV KYWLIVNFIY HDILNFNTTS
     FPIEQYEKFF QRDQNSLPSS ANFIESIDSP IEEIDPLIGI NKPILLLLGQ VTNLTRFLQT
     MEQEEMLEHG DKILSLQVEI YKLQPSLMAL EHLDDEKKFY YLELFEIMKI STLMFFQLTL
     LKIDKDSLEL QILRNKLDSK LDKVIGTFLE GSLCFPLFIY GVCIQVEDME KKIDLEAKFD
     DILKRYKCYN FQNARLLIRK IWQNEADGIS EHDLVHMIDE LDYNINFA