ID   UGA4E_AGRRK             Reviewed;         334 AA.
AC   B9J8R3;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=UDP-glucuronate 4-epimerase {ECO:0000303|Ref.2};
DE            Short=UGA4E {ECO:0000303|Ref.2};
DE            EC=5.1.3.6 {ECO:0000269|Ref.2};
DE   AltName: Full=ArUGAE {ECO:0000303|Ref.2};
GN   Name=lpsL {ECO:0000312|EMBL:ACM27451.1};
GN   OrderedLocusNames=Arad_3521 {ECO:0000312|EMBL:ACM27451.1};
OS   Agrobacterium radiobacter (strain K84 / ATCC BAA-868).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=311403;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K84 / ATCC BAA-868;
RX   PubMed=19251847; DOI=10.1128/jb.01779-08;
RA   Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K.,
RA   Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L.,
RA   Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A.,
RA   Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M.,
RA   Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N.,
RA   Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W.;
RT   "Genome sequences of three Agrobacterium biovars help elucidate the
RT   evolution of multichromosome genomes in bacteria.";
RL   J. Bacteriol. 191:2501-2511(2009).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   DOI=10.3390/catal10020222;
RA   Gevaert O., Van Overtveldt S., Da Costa M., Beerens K., Desmet T.;
RT   "Novel insights into the existence of the putative UDP-glucuronate 5-
RT   epimerase specificity.";
RL   Catalysts 10:222-222(2020).
CC   -!- FUNCTION: Catalyzes the interconversion of UDP-D-glucuronic acid (UDP-
CC       GlcA) and UDP-D-galacturonic acid (UDP-GalA). {ECO:0000269|Ref.2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucuronate = UDP-alpha-D-galacturonate;
CC         Xref=Rhea:RHEA:11404, ChEBI:CHEBI:57635, ChEBI:CHEBI:58052;
CC         EC=5.1.3.6; Evidence={ECO:0000269|Ref.2};
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC         Evidence={ECO:0000250|UniProtKB:Q14376};
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; CP000628; ACM27451.1; -; Genomic_DNA.
DR   RefSeq; WP_012652135.1; NC_011985.1.
DR   AlphaFoldDB; B9J8R3; -.
DR   SMR; B9J8R3; -.
DR   STRING; 311403.Arad_3521; -.
DR   EnsemblBacteria; ACM27451; ACM27451; Arad_3521.
DR   KEGG; ara:Arad_3521; -.
DR   eggNOG; COG0451; Bacteria.
DR   HOGENOM; CLU_007383_1_7_5; -.
DR   OMA; EPIKVFN; -.
DR   OrthoDB; 1180629at2; -.
DR   Proteomes; UP000001600; Chromosome 1.
DR   GO; GO:0050378; F:UDP-glucuronate 4-epimerase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF01370; Epimerase; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Isomerase; NAD; Reference proteome.
FT   CHAIN           1..334
FT                   /note="UDP-glucuronate 4-epimerase"
FT                   /id="PRO_0000450327"
FT   ACT_SITE        152
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q14376"
FT   BINDING         10..12
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q14376"
FT   BINDING         156
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q14376"
SQ   SEQUENCE   334 AA;  37237 MW;  8D37D48314A5C2C4 CRC64;
     MRYLITGTAG FIGFHLAKRL LDDGHFVVGF DGMTPYYDVK LKEKRTAILA RSNGFKAVTG
     MLEDKAALDH AAELAEPDVI VHLAAQAGVR YSLENPRSYV DSNLVGSFNV LELARSIQPK
     HLLLASTSSV YGANEKIPFA ESDKADEQMT IYAATKKSME LMAHSYAHLF HIPTTVFRFF
     TVYGPWGRPD MALFKFVEAI KHDRPIEIYG EGKMSRDFTY IDDLVEGIVK LIGVIPSEEN
     RVVSDTISDT LSKNAPFRIV NIGGGQPVGL MAFVETIEAM LGKRAIRHML PMQPGDVHNT
     YAVPDLLVAL TGFKPQIEVD AGVRRFVEWY QENY