UGA4_YEAST
ID UGA4_YEAST Reviewed; 571 AA.
AC P32837; D6VRE4;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=GABA-specific permease;
DE AltName: Full=GABA-specific transport protein;
GN Name=UGA4; OrderedLocusNames=YDL210W; ORFNames=D1037;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sigma 1278B;
RX PubMed=8455553; DOI=10.1007/bf00282779;
RA Andre B., Hein C., Grenson M., Jauniaux J.-C.;
RT "Cloning and expression of the UGA4 gene coding for the inducible GABA-
RT specific transport protein of Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 237:17-25(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=14985124; DOI=10.1016/j.bbrc.2004.01.162;
RA Uemura T., Tomonari Y., Kashiwagi K., Igarashi K.;
RT "Uptake of GABA and putrescine by UGA4 on the vacuolar membrane in
RT Saccharomyces cerevisiae.";
RL Biochem. Biophys. Res. Commun. 315:1082-1087(2004).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Required for high-affinity, high-specificity GABA transport.
CC Also transports putrescine. {ECO:0000269|PubMed:14985124}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:14985124};
CC Multi-pass membrane protein {ECO:0000269|PubMed:14985124}.
CC -!- INDUCTION: In the presence of GABA.
CC -!- MISCELLANEOUS: It requires NPR (nitrogen permease reactivator protein)
CC for its full activity.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.
CC {ECO:0000305}.
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DR EMBL; X66472; CAA47101.1; -; Genomic_DNA.
DR EMBL; X99000; CAA67481.1; -; Genomic_DNA.
DR EMBL; Z74258; CAA98788.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11654.1; -; Genomic_DNA.
DR PIR; S30253; S30253.
DR RefSeq; NP_010071.1; NM_001180270.1.
DR AlphaFoldDB; P32837; -.
DR SMR; P32837; -.
DR BioGRID; 31836; 81.
DR DIP; DIP-1857N; -.
DR IntAct; P32837; 7.
DR MINT; P32837; -.
DR STRING; 4932.YDL210W; -.
DR TCDB; 2.A.3.4.3; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; P32837; -.
DR PRIDE; P32837; -.
DR EnsemblFungi; YDL210W_mRNA; YDL210W; YDL210W.
DR GeneID; 851317; -.
DR KEGG; sce:YDL210W; -.
DR SGD; S000002369; UGA4.
DR VEuPathDB; FungiDB:YDL210W; -.
DR eggNOG; KOG1289; Eukaryota.
DR HOGENOM; CLU_004495_0_3_1; -.
DR InParanoid; P32837; -.
DR OMA; AFGVKLM; -.
DR BioCyc; YEAST:G3O-29592-MON; -.
DR PRO; PR:P32837; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P32837; protein.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015495; F:gamma-aminobutyric acid:proton symporter activity; IGI:SGD.
DR GO; GO:0015489; F:putrescine transmembrane transporter activity; IMP:SGD.
DR GO; GO:0015812; P:gamma-aminobutyric acid transport; IMP:SGD.
DR GO; GO:0015847; P:putrescine transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IGI:SGD.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR004840; Amoino_acid_permease_CS.
DR Pfam; PF13520; AA_permease_2; 1.
DR PROSITE; PS00218; AMINO_ACID_PERMEASE_1; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..571
FT /note="GABA-specific permease"
FT /id="PRO_0000054161"
FT TOPO_DOM 1..73
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..105
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..153
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..198
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..271
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..312
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..364
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 386..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..441
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..482
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 504..514
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 536..571
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 571 AA; 61873 MW; 051B5828654EC784 CRC64;
MSMSSKNENK ISVEQRISTD IGQAYQLQGL GSNLRSIRSK TGAGEVNYID AAKSVNDNQL
LAEIGYKQEL KRQFSTLQVF GIAFSIMGLL PSIASVMGGG LGGGPATLVW GWFVAAFFIL
LVGITMAEHA SSIPTAGGLY YWTYYYAPEG YKEIISFIIG CSNSLALAAG VCSIDYGLAE
EIAAAVTLTK DGNFEVTSGK LYGIFAGAVV VMCICTCVAS GAIARLQTLS IFANLFIIVL
LFIALPIGTK HRMGGFNDGD FIFGKYENLS DWNNGWQFCL AGFMPAVWTI GSFDSCVHQS
EEAKDAKKSV PIGIISSIAV CWILGWLIII CLMACINPDI DSVLDSKYGF ALAQIIYDSL
GKKWAIAFMS LIAFCQFLMG ASITTAVSRQ VWAFSRDNGL PLSKYIKRVD SKYSVPFFAI
LAACVGSLIL GLLCLIDDAA TDALFSLAVA GNNLAWSTPT VFRLTSGRDL FRPGPFYLGK
IWSPIVAWTG VAFQLFIIIL VMFPSQQHGI TKSTMNYACV IGPGIWILAG IYYKVYKKKY
YHGPATNLSD DDYTEAVGAD VIDTIMSKQE P
