UGE1_SCHPO
ID UGE1_SCHPO Reviewed; 355 AA.
AC Q9Y7X5;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=UDP-glucose 4-epimerase uge1;
DE EC=5.1.3.2;
DE AltName: Full=Galactowaldenase;
GN Name=uge1; ORFNames=SPBC365.14c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP DISRUPTION PHENOTYPE.
RX PubMed=20537132; DOI=10.1186/gb-2010-11-6-r60;
RA Han T.X., Xu X.Y., Zhang M.J., Peng X., Du L.L.;
RT "Global fitness profiling of fission yeast deletion strains by barcode
RT sequencing.";
RL Genome Biol. 11:R60.1-R60.13(2010).
RN [3]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=19942659; DOI=10.1099/mic.0.035279-0;
RA Suzuki S., Matsuzawa T., Nukigi Y., Takegawa K., Tanaka N.;
RT "Characterization of two different types of UDP-glucose/-galactose 4-
RT epimerase involved in galactosylation in fission yeast.";
RL Microbiology 156:708-718(2010).
CC -!- FUNCTION: Major UDP-glucose/-galactose 4-epimerase under glucose-rich
CC conditions involved in protein galactosylation.
CC {ECO:0000269|PubMed:19942659}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC EC=5.1.3.2; Evidence={ECO:0000269|PubMed:19942659};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC -!- DISRUPTION PHENOTYPE: Leads to sensitivity to thiabendazole and
CC microtubule-depolymerizing drugs. {ECO:0000269|PubMed:20537132}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000305}.
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DR EMBL; CU329671; CAB44766.1; -; Genomic_DNA.
DR PIR; T40321; T40321.
DR RefSeq; NP_596043.1; NM_001021953.2.
DR AlphaFoldDB; Q9Y7X5; -.
DR SMR; Q9Y7X5; -.
DR BioGRID; 277454; 21.
DR STRING; 4896.SPBC365.14c.1; -.
DR MaxQB; Q9Y7X5; -.
DR PaxDb; Q9Y7X5; -.
DR PRIDE; Q9Y7X5; -.
DR EnsemblFungi; SPBC365.14c.1; SPBC365.14c.1:pep; SPBC365.14c.
DR GeneID; 2540938; -.
DR KEGG; spo:SPBC365.14c; -.
DR PomBase; SPBC365.14c; uge1.
DR VEuPathDB; FungiDB:SPBC365.14c; -.
DR eggNOG; KOG1371; Eukaryota.
DR HOGENOM; CLU_007383_1_10_1; -.
DR InParanoid; Q9Y7X5; -.
DR OMA; GEHLICN; -.
DR PhylomeDB; Q9Y7X5; -.
DR BRENDA; 5.1.3.2; 5613.
DR UniPathway; UPA00214; -.
DR PRO; PR:Q9Y7X5; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; ISO:PomBase.
DR GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IMP:PomBase.
DR GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0042125; P:protein galactosylation; IMP:PomBase.
DR GO; GO:0052574; P:UDP-galactose biosynthetic process; IMP:PomBase.
DR CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR005886; UDP_G4E.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01179; galE; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Galactose metabolism; Isomerase; NAD;
KW Reference proteome.
FT CHAIN 1..355
FT /note="UDP-glucose 4-epimerase uge1"
FT /id="PRO_0000318143"
FT BINDING 8..39
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 355 AA; 39234 MW; F8B24D00CD7AF7E3 CRC64;
MTGVHEGTVL VTGGAGYIGS HTCVVLLEKG YDVVIVDNLC NSRVEAVHRI EKLTGKKVIF
HQVDLLDEPA LDKVFANQNI SAVIHFAGLK AVGESVQVPL SYYKNNISGT INLIECMKKY
NVRDFVFSSS ATVYGDPTRP GGTIPIPESC PREGTSPYGR TKLFIENIIE DETKVNKSLN
AALLRYFNPG GAHPSGELGE DPLGIPNNLL PYIAQVAVGR LDHLNVFGDD YPTSDGTPIR
DYIHVCDLAE AHVAALDYLR QHFVSCRPWN LGSGTGSTVF QVLNAFSKAV GRDLPYKVTP
RRAGDVVNLT ANPTRANEEL KWKTSRSIYE ICVDTWRWQQ KYPYGFDLTH TKTYK
