ID   UGE4_ORYSJ              Reviewed;         369 AA.
AC   Q6K2E1; A0A0P0XLE5;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=UDP-glucose 4-epimerase 4;
DE            Short=OsUGE-4;
DE            EC=5.1.3.2;
DE   AltName: Full=UDP-galactose 4-epimerase 4;
GN   Name=UGE-4; OrderedLocusNames=Os09g0323000, LOC_Os09g15420;
GN   ORFNames=OsJ_28859, OSJNBb0024J13.13-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=16266295; DOI=10.1042/bj20051329;
RA   Zhang Q., Hrmova M., Shirley N.J., Lahnstein J., Fincher G.B.;
RT   "Gene expression patterns and catalytic properties of UDP-D-glucose 4-
RT   epimerases from barley (Hordeum vulgare L.).";
RL   Biochem. J. 394:115-124(2006).
CC   -!- FUNCTION: Catalyzes the interconversion between UDP-glucose and UDP-
CC       galactose. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; AP005879; BAD23675.1; -; Genomic_DNA.
DR   EMBL; AP008215; BAF24783.1; -; Genomic_DNA.
DR   EMBL; AP014965; BAT07457.1; -; Genomic_DNA.
DR   EMBL; CM000146; EEE69457.1; -; Genomic_DNA.
DR   EMBL; AK121426; BAH00483.1; -; mRNA.
DR   RefSeq; XP_015611994.1; XM_015756508.1.
DR   AlphaFoldDB; Q6K2E1; -.
DR   SMR; Q6K2E1; -.
DR   STRING; 4530.OS09T0323000-01; -.
DR   PaxDb; Q6K2E1; -.
DR   PRIDE; Q6K2E1; -.
DR   EnsemblPlants; Os09t0323000-01; Os09t0323000-01; Os09g0323000.
DR   GeneID; 4346723; -.
DR   Gramene; Os09t0323000-01; Os09t0323000-01; Os09g0323000.
DR   KEGG; osa:4346723; -.
DR   eggNOG; KOG1371; Eukaryota.
DR   HOGENOM; CLU_007383_1_10_1; -.
DR   InParanoid; Q6K2E1; -.
DR   OMA; NWYGRTK; -.
DR   OrthoDB; 662484at2759; -.
DR   PlantReactome; R-OSA-1119452; Galactose degradation II.
DR   UniPathway; UPA00214; -.
DR   Proteomes; UP000000763; Chromosome 9.
DR   Proteomes; UP000007752; Chromosome 9.
DR   Proteomes; UP000059680; Chromosome 9.
DR   ExpressionAtlas; Q6K2E1; baseline and differential.
DR   Genevisible; Q6K2E1; OS.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR   CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005886; UDP_G4E.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01179; galE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Galactose metabolism; Isomerase; NAD;
KW   Reference proteome.
FT   CHAIN           1..369
FT                   /note="UDP-glucose 4-epimerase 4"
FT                   /id="PRO_0000422191"
FT   ACT_SITE        168
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         19..50
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         144
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   369 AA;  39647 MW;  EC4985E8265BCD18 CRC64;
     MAGGEVVAVA AAAAGTSRTV LVTGGAGYIG SHTVLQLLAA GFRVVVADSL GNSSELAVRR
     VAALAGDKAR NLSLHKVDIR DKGGLEKVFS STRFDAVVHF AGLKAVGESV QKPLLYYDHN
     VAGTIILLEV MAAHGCKKLV FSSSAAVYGS PKNSPCTEEF PLTPHNPYGR TKLIAEEICR
     DIYHSDSEWS IILLRYFNPV GAHPSGYLGE DPCGIPNNLM PFVQQVAVGR RPSLTIFGND
     YATKDGTGVR DYIHVVDLAE GHIAALRKLF ESSIGCQAYN LGTGKGTSVL EIVNAFEKVS
     GKKIPLVIGP RRPGDAEILF SSAAKAEREF KWKAKYGIEE MCRDQWNWAS KNPFGYASPD
     STKQNGHSH