ID   UGNO_METMP              Reviewed;         311 AA.
AC   Q6M0B9;
DT   04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=UDP-N-acetylglucosamine 3-dehydrogenase {ECO:0000305};
DE            EC=1.1.1.374 {ECO:0000269|PubMed:18634748};
DE   AltName: Full=UDP-GlcNAc oxidoreductase {ECO:0000303|PubMed:18634748};
DE   AltName: Full=UDP-N-acetyl-alpha-D-glucosamine:NAD(+) 3-oxidoreductase {ECO:0000305};
GN   OrderedLocusNames=MMP0352 {ECO:0000312|EMBL:CAF29908.1};
OS   Methanococcus maripaludis (strain S2 / LL).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=267377;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S2 / LL;
RX   PubMed=15466049; DOI=10.1128/jb.186.20.6956-6969.2004;
RA   Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J.,
RA   Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M.,
RA   Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A.,
RA   Moore B.C., Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D.,
RA   Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W.,
RA   Olson M.V., Leigh J.A.;
RT   "Complete genome sequence of the genetically tractable hydrogenotrophic
RT   methanogen Methanococcus maripaludis.";
RL   J. Bacteriol. 186:6956-6969(2004).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND SUBUNIT.
RC   STRAIN=S2 / LL;
RX   PubMed=18634748; DOI=10.1016/j.ab.2008.06.034;
RA   Namboori S.C., Graham D.E.;
RT   "Enzymatic analysis of uridine diphosphate N-acetyl-D-glucosamine.";
RL   Anal. Biochem. 381:94-100(2008).
CC   -!- FUNCTION: Catalyzes the NAD(+)-dependent oxidation of UDP-GlcNAc.
CC       Highly specific for UDP-GlcNAc, does not catalyze the oxidation of the
CC       substrate analogs UDP-Glc, UDP-GalNAc, N-acetylglucosamine and
CC       glucosamine. {ECO:0000269|PubMed:18634748}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + UDP-N-acetyl-alpha-D-glucosamine = H(+) + NADH + UDP-
CC         2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose;
CC         Xref=Rhea:RHEA:42660, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57705, ChEBI:CHEBI:57945, ChEBI:CHEBI:78981;
CC         EC=1.1.1.374; Evidence={ECO:0000269|PubMed:18634748};
CC   -!- ACTIVITY REGULATION: Oxidoreductase activity is reduced by 50% in the
CC       presence of 2 mM UDP, 2 mM UDP-Glc or 3 mM UDP-GalNAc. Activity is
CC       unaffected by metal ions. {ECO:0000269|PubMed:18634748}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=180 uM for UDP-GalNAc {ECO:0000269|PubMed:18634748};
CC         KM=180 uM for NAD(+) {ECO:0000269|PubMed:18634748};
CC         Vmax=1.5 umol/min/mg enzyme toward UDP-GalNAc
CC         {ECO:0000269|PubMed:18634748};
CC         Vmax=1.6 umol/min/mg enzyme toward NAD(+)
CC         {ECO:0000269|PubMed:18634748};
CC         Note=kcat is 0.9 sec(-) with UDP-GalNAc. kcat is 0.99 sec(-1) with
CC         NAD(+). {ECO:0000269|PubMed:18634748};
CC   -!- SUBUNIT: Homooctamer. {ECO:0000269|PubMed:18634748}.
CC   -!- SIMILARITY: Belongs to the Gfo/Idh/MocA family. {ECO:0000305}.
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DR   EMBL; BX950229; CAF29908.1; -; Genomic_DNA.
DR   RefSeq; WP_011170296.1; NC_005791.1.
DR   AlphaFoldDB; Q6M0B9; -.
DR   SMR; Q6M0B9; -.
DR   STRING; 267377.MMP0352; -.
DR   EnsemblBacteria; CAF29908; CAF29908; MMP0352.
DR   GeneID; 2762080; -.
DR   KEGG; mmp:MMP0352; -.
DR   PATRIC; fig|267377.15.peg.355; -.
DR   eggNOG; arCOG01622; Archaea.
DR   HOGENOM; CLU_023194_10_0_2; -.
DR   OMA; VPDMTRW; -.
DR   OrthoDB; 39139at2157; -.
DR   BioCyc; MMAR267377:MMP_RS01885-MON; -.
DR   BRENDA; 1.1.1.374; 3262.
DR   Proteomes; UP000000590; Chromosome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004104; Gfo/Idh/MocA-like_OxRdtase_C.
DR   InterPro; IPR000683; Gfo/Idh/MocA-like_OxRdtase_N.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF01408; GFO_IDH_MocA; 1.
DR   Pfam; PF02894; GFO_IDH_MocA_C; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..311
FT                   /note="UDP-N-acetylglucosamine 3-dehydrogenase"
FT                   /id="PRO_0000431699"
SQ   SEQUENCE   311 AA;  34363 MW;  276CD759789E9147 CRC64;
     MLKVAVVGIG VMGYNHARIY NELQKDGNVE LVGLSDMNKD RLNEVSEEFG VKAFSNYMDL
     LEEDLDAVSI VVPTFLHKEV ALPFIKKGIS VLIEKPIADT IENANEIIAE AEKNNVILSV
     GHVERFNPAV LKLKEHVEKG NLGDIVTMTA KRVGPMTTRI ADAGVILDLA VHDIDVMAFL
     ANSKVKHVHA LAKNVKHPNN NAEDYALIIS SFENSIDGII EVNRLTPHKT RTLNIVGTKG
     IAYLDYMNQD LTLYDEEWVK TAKINKEEPL KKEIAHFVDC VQNNKQPLVT GLDGLNALET
     AIYALKSSNG N