UGNT1_ARATH
ID UGNT1_ARATH Reviewed; 344 AA.
AC A8MRY9; O49364;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=UDP-N-acetylglucosamine transporter UGNT1 {ECO:0000305};
DE Short=UDP-GlcNAc transporter 1 {ECO:0000303|PubMed:30224661};
DE AltName: Full=Nucleotide-sugar uncharacterized transporter 3 {ECO:0000305};
GN Name=UGNT1 {ECO:0000303|PubMed:30224661};
GN OrderedLocusNames=At4g32272 {ECO:0000312|Araport:AT4G32272};
GN ORFNames=F10M6.90 {ECO:0000312|EMBL:CAA16963.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=30224661; DOI=10.1038/s41477-018-0235-5;
RA Ebert B., Rautengarten C., McFarlane H.E., Rupasinghe T., Zeng W., Ford K.,
RA Scheller H.V., Bacic A., Roessner U., Persson S., Heazlewood J.L.;
RT "A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans
RT and sphingolipids.";
RL Nat. Plants 4:792-801(2018).
CC -!- FUNCTION: Mediates the transport of UDP-N-acetylglucosamine (UDP-
CC GlcNAc) across the Golgi apparatus membrane (PubMed:30224661). Delivers
CC an essential substrate for the maturation of N-glycans and the GlcNAc-
CC containing glycosyl inositol phosphorylceramide (GIPC) class of
CC sphingolipids in the Golgi apparatus (PubMed:30224661).
CC {ECO:0000269|PubMed:30224661}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=9 uM for UDP-N-acetyl-D-glucosamine {ECO:0000269|PubMed:30224661};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:30224661}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=A8MRY9-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC siliques. {ECO:0000269|PubMed:30224661}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants have increased levels of high mannose N-
CC glycans, decreased levels of complex N-glycans, and are insensitive to
CC root growth inhibition by salt stress. {ECO:0000269|PubMed:30224661}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. UGnT (TC 2.A.7.15)
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AY075601; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA16963.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79945.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL021811; CAA16963.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161580; CAB79945.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86030.1; -; Genomic_DNA.
DR EMBL; AY075601; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T05401; T05401.
DR RefSeq; NP_001078479.1; NM_001085010.2. [A8MRY9-1]
DR AlphaFoldDB; A8MRY9; -.
DR SMR; A8MRY9; -.
DR STRING; 3702.AT4G32272.2; -.
DR PRIDE; A8MRY9; -.
DR ProteomicsDB; 249076; -. [A8MRY9-1]
DR EnsemblPlants; AT4G32272.1; AT4G32272.1; AT4G32272. [A8MRY9-1]
DR GeneID; 5008177; -.
DR Gramene; AT4G32272.1; AT4G32272.1; AT4G32272. [A8MRY9-1]
DR KEGG; ath:AT4G32272; -.
DR Araport; AT4G32272; -.
DR eggNOG; KOG1444; Eukaryota.
DR HOGENOM; CLU_040726_0_0_1; -.
DR OMA; TVKFPDF; -.
DR PhylomeDB; A8MRY9; -.
DR PRO; PR:A8MRY9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; A8MRY9; baseline and differential.
DR GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005461; F:UDP-glucuronic acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005463; F:UDP-N-acetylgalactosamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Golgi apparatus; Membrane; Reference proteome;
KW Stress response; Sugar transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..344
FT /note="UDP-N-acetylglucosamine transporter UGNT1"
FT /id="PRO_0000439530"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 344 AA; 37640 MW; FCB6A5B286EA2241 CRC64;
MRNNPVLPVS DPPLAGENDS DGKGVDDRLF KGSAMTKRGA YAALSYMACA VMLVLFNKAA
LSSYDFPCVN VITLFQMVSS SLFLYALRRR KIISFTAADS FSIDSASTFV PVKTLFHTLP
LAIAYLLYML ASMASVRGVN VPMYTTLRRT TVAFTMVIEY MLTGQRYTRS IIGSVGIILL
GAFFAGARDL SFDFYGYGVV FLANISTAVY LATIARTGKS SGLNSFGLMW SNGIICGPIL
MIWTFICGDL EKTINFPHLL TPGFMVVLLC SCVLAFVLNY CIFLNTTLNS ALTQTICGNM
KDLFTVGLGW MLFGGLPFDL MNVIGQLFGF FGSGLYAYYK IIGR
