ID   UGT13_PANGI             Reviewed;         472 AA.
AC   A0A0K0PVL3;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   11-NOV-2015, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=UDP-glucosyltransferase 103 {ECO:0000303|PubMed:26032089};
DE            Short=UGTPg103 {ECO:0000303|PubMed:26032089};
DE            EC=2.4.1.366 {ECO:0000305|PubMed:26032089};
GN   Name=UGT103 {ECO:0000303|PubMed:26032089};
OS   Panax ginseng (Korean ginseng).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Araliaceae; Panax.
OX   NCBI_TaxID=4054;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF THR-142; SER-186
RP   AND ARG-338.
RX   PubMed=26032089; DOI=10.1016/j.molp.2015.05.010;
RA   Wei W., Wang P., Wei Y., Liu Q., Yang C., Zhao G., Yue J., Yan X., Zhou Z.;
RT   "Characterization of Panax ginseng UDP-glycosyltransferases catalyzing
RT   protopanaxatriol and biosyntheses of bioactive ginsenosides F1 and Rh1 in
RT   metabolically engineered yeasts.";
RL   Mol. Plant 8:1412-1424(2015).
CC   -!- FUNCTION: Probable component of the triterpene saponins (e.g.
CC       ginsenosides) biosynthetic pathway (PubMed:26032089). No detectable
CC       activity toward protopanaxatriol (PPT) (PubMed:26032089).
CC       {ECO:0000269|PubMed:26032089}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(20S)-ginsenoside F1 + UDP-alpha-D-glucose = (20S)-ginsenoside
CC         Rg1 + H(+) + UDP; Xref=Rhea:RHEA:58008, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:67987,
CC         ChEBI:CHEBI:77150; EC=2.4.1.366;
CC         Evidence={ECO:0000305|PubMed:26032089};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:58009;
CC         Evidence={ECO:0000305|PubMed:26032089};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; KP795116; AKQ76391.1; -; mRNA.
DR   AlphaFoldDB; A0A0K0PVL3; -.
DR   SMR; A0A0K0PVL3; -.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Isoprene biosynthesis; Transferase.
FT   CHAIN           1..472
FT                   /note="UDP-glucosyltransferase 103"
FT                   /id="PRO_0000446968"
FT   BINDING         279
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         343..344
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         361..369
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   BINDING         383..386
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250|UniProtKB:Q9M156"
FT   MUTAGEN         142
FT                   /note="T->A: Gained ability to transfer a glucose residue
FT                   to the C6-OH of protopanaxatriol (PPT); when associated
FT                   with L-186 and G-338."
FT                   /evidence="ECO:0000269|PubMed:26032089"
FT   MUTAGEN         186
FT                   /note="S->L: Gained ability to transfer a glucose residue
FT                   to the C6-OH of protopanaxatriol (PPT); when associated
FT                   with T-142 and G-338."
FT                   /evidence="ECO:0000269|PubMed:26032089"
FT   MUTAGEN         338
FT                   /note="R->G: Gained ability to transfer a glucose residue
FT                   to the C6-OH of protopanaxatriol (PPT); when associated
FT                   with T-142 and L-186."
FT                   /evidence="ECO:0000269|PubMed:26032089"
SQ   SEQUENCE   472 AA;  53204 MW;  C80409971D6C54D3 CRC64;
     MKSELIFLPV PAFGHLVGMV EMAKLFISRH ENLSVTVLIS KFFIDTGIDN YNKSLLAKPT
     PRLTIINLPE IDPQKYLLKP RCAIFPSLIE NQKTHVRDVM SRMTQSESTR IVGLLADILF
     VDIFDIADEF NVPTYVYSPA GTGFLGLAFH LQTLNDDKKQ DVTEFRNSDT ELLVPSFANP
     VPAEFSPSIF LEKDGRHDVL LSLYRRCREA KGIIVNTFEE LEPYAINSLR MDSMIPPIYP
     VGPILNLNGE GQNSDEAAVI LGWLDDQPPS SVVFLCFGSF GSFPENQVKE IAMGLERSGH
     RFLWSLRPCI SEGETTLQLK YSNLELPAGF LDRTSCVRKV IGWAPQMAVL AHEAVGGFVS
     HCGWNSVLES VWYGMPVATW PMYGEQQLNA FEMVKELGLA VEIEVDYRNE YNKSDFIVKA
     DEIETKIKKL MMDGKNSKIR KKVKEMKEKS RVAMSENGSS YTSLAKLFEE IM