UGT1_GARJA
ID UGT1_GARJA Reviewed; 474 AA.
AC F8WKW0;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 03-AUG-2022, entry version 28.
DE RecName: Full=Crocetin glucosyltransferase, chloroplastic;
DE EC=2.4.1.271;
DE AltName: Full=UDP-glucose glucosyltransferase 1;
DE Short=GjUGT1;
DE AltName: Full=UDP-glycosyltransferase 75L6;
DE Flags: Precursor;
GN Name=UGT75L6; Synonyms=UGT1;
OS Gardenia jasminoides (Cape jasmine) (Gardenia augusta).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex;
OC Gardenieae - Pavetteae clade; Gardenieae; Gardenia.
OX NCBI_TaxID=114476;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=21799001; DOI=10.1074/jbc.m111.242586;
RA Nagatoshi M., Terasaka K., Nagatsu A., Mizukami H.;
RT "Iridoid-specific Glucosyltransferase from Gardenia jasminoides.";
RL J. Biol. Chem. 286:32866-32874(2011).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP SPECIFICITY.
RX PubMed=22569263; DOI=10.1016/j.febslet.2012.03.003;
RA Nagatoshi M., Terasaka K., Owaki M., Sota M., Inukai T., Nagatsu A.,
RA Mizukami H.;
RT "UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin
RT in Gardenia jasminoides.";
RL FEBS Lett. 586:1055-1061(2012).
CC -!- FUNCTION: Glucosyltransferase acting on a broad range of substrates,
CC including crocetin, 4-coumaric acid, caffeic acid and ferulic acid. No
CC activity with indol-3-acetic acid, bixin and norbixin, and no formation
CC of O-glucosides. Involved with UGT94E5 in sequential glycosylation of
CC crocetin to crocin (bis(beta-D-gentiobiosyl) crocetin).
CC {ECO:0000269|PubMed:22569263}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=crocetin + UDP-alpha-D-glucose = beta-D-glucosyl crocetin +
CC UDP; Xref=Rhea:RHEA:31463, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:62766, ChEBI:CHEBI:62767; EC=2.4.1.271;
CC Evidence={ECO:0000269|PubMed:22569263};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-glucosyl crocetin + UDP-alpha-D-glucose = bis(beta-D-
CC glucosyl) crocetin + UDP; Xref=Rhea:RHEA:31467, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:62766, ChEBI:CHEBI:62768;
CC EC=2.4.1.271; Evidence={ECO:0000269|PubMed:22569263};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-gentiobiosyl crocetin + UDP-alpha-D-glucose = beta-D-
CC gentiobiosyl beta-D-glucosyl crocetin + UDP; Xref=Rhea:RHEA:31471,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:62770,
CC ChEBI:CHEBI:62771; EC=2.4.1.271;
CC Evidence={ECO:0000269|PubMed:22569263};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.46 mM for crocetin {ECO:0000269|PubMed:22569263};
CC KM=1.5 mM for 4-coumaric acid {ECO:0000269|PubMed:22569263};
CC KM=2.5 mM for caffeic acid {ECO:0000269|PubMed:22569263};
CC KM=1.7 mM for ferulic acid {ECO:0000269|PubMed:22569263};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:22569263}.
CC -!- MISCELLANEOUS: Crocin is a yellow pigment that accumulates during fruit
CC development. No other crocetin glucosyl esters detected in neither
CC flowers, leaves, nor stems. No correlation between mRNA expression and
CC pigment accumulation (PubMed:22569263). {ECO:0000305|PubMed:22569263}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB555731; BAK55736.1; -; mRNA.
DR AlphaFoldDB; F8WKW0; -.
DR SMR; F8WKW0; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR KEGG; ag:BAK55736; -.
DR BioCyc; MetaCyc:MON-18779; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProt.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Glycosyltransferase; Plastid; Transferase; Transit peptide.
FT TRANSIT 1..45
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 46..474
FT /note="Crocetin glucosyltransferase, chloroplastic"
FT /id="PRO_0000430136"
FT BINDING 286
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 344..346
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 361..369
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 383..386
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 474 AA; 53008 MW; 7C09637AEEA5645D CRC64;
MVQQRHVLLI TYPAQGHINP ALQFAQRLLR MGIQVTLATS VYALSRMKKS SGSTPKGLTF
ATFSDGYDDG FRPKGVDHTE YMSSLAKQGS NTLRNVINTS ADQGCPVTCL VYTLLLPWAA
TVARECHIPS ALLWIQPVAV MDIYYYYFRG YEDDVKNNSN DPTWSIQFPG LPSMKAKDLP
SFILPSSDNI YSFALPTFKK QLETLDEEER PKVLVNTFDA LEPQALKAIE SYNLIAIGPL
TPSAFLDGKD PSETSFSGDL FQKSKDYKEW LNSRPAGSVV YVSFGSLLTL PKQQMEEIAR
GLLKSGRPFL WVIRAKENGE EEKEEDRLIC MEELEEQGMI VPWCSQIEVL THPSLGCFVT
HCGWNSTLET LVCGVPVVAF PHWTDQGTNA KLIEDVWETG VRVVPNEDGT VESDEIKRCI
ETVMDDGEKG VELKRNAKKW KELAREAMQE DGSSDKNLKA FVEDAGKGYQ AESN
