UGT1_PANGI
ID UGT1_PANGI Reviewed; 475 AA.
AC A0A068J840;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2014, sequence version 1.
DT 03-AUG-2022, entry version 28.
DE RecName: Full=UDP-glycosyltransferase 1 {ECO:0000303|PubMed:24603359};
DE Short=UGTPg1 {ECO:0000303|PubMed:24603359, ECO:0000303|PubMed:29509695};
DE EC=2.4.1.363 {ECO:0000269|PubMed:24603359, ECO:0000269|PubMed:26032089};
GN Name=UGT1 {ECO:0000303|PubMed:24603359};
OS Panax ginseng (Korean ginseng).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Araliaceae; Panax.
OX NCBI_TaxID=4054;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
RX PubMed=24603359; DOI=10.1038/cr.2014.28;
RA Yan X., Fan Y., Wei W., Wang P., Liu Q., Wei Y., Zhang L., Zhao G., Yue J.,
RA Zhou Z.;
RT "Production of bioactive ginsenoside compound K in metabolically engineered
RT yeast.";
RL Cell Res. 24:770-773(2014).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF ALA-10; ILE-13; LEU-38;
RP ALA-40; HIS-82; PHE-85; THR-134; HIS-144 AND GLN-388.
RX PubMed=26032089; DOI=10.1016/j.molp.2015.05.010;
RA Wei W., Wang P., Wei Y., Liu Q., Yang C., Zhao G., Yue J., Yan X., Zhou Z.;
RT "Characterization of Panax ginseng UDP-glycosyltransferases catalyzing
RT protopanaxatriol and biosyntheses of bioactive ginsenosides F1 and Rh1 in
RT metabolically engineered yeasts.";
RL Mol. Plant 8:1412-1424(2015).
RN [3]
RP REVIEW.
RX PubMed=29378087; DOI=10.1002/bab.1649;
RA Lu J., Li J., Wang S., Yao L., Liang W., Wang J., Gao W.;
RT "Advances in ginsenoside biosynthesis and metabolic regulation.";
RL Biotechnol. Appl. Biochem. 65:514-522(2018).
RN [4]
RP REVIEW, AND NOMENCLATURE.
RX PubMed=29509695; DOI=10.3390/molecules23030589;
RA Yang J.-L., Hu Z.-F., Zhang T.-T., Gu A.-D., Gong T., Zhu P.;
RT "Progress on the studies of the key enzymes of ginsenoside biosynthesis.";
RL Molecules 23:0-0(2018).
CC -!- FUNCTION: Component of the dammarane-type triterpene saponins (e.g.
CC ginsenosides or panaxosides) biosynthetic pathway (PubMed:26032089,
CC PubMed:29378087). Glycosyltransferase that catalyzes the biosynthesis
CC of ginsenoside F1 from protopanaxatriol (PPT) (PubMed:26032089).
CC Triggers C20-OH glycosylation of ginsenoside Rg3 to produce ginsenoside
CC Rd (PubMed:26032089). Mediates the conversion of protopanaxadiol (PPD)
CC to the ginsenoside compound K (PubMed:24603359, PubMed:26032089).
CC catalyzes the production of 20S-O-beta-(D-glucosyl)-dammarenediol II
CC form dammarenediol II (DM) (PubMed:24603359).
CC {ECO:0000269|PubMed:24603359, ECO:0000269|PubMed:26032089,
CC ECO:0000303|PubMed:29378087}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(20S)-protopanaxadiol + UDP-alpha-D-glucose = (20S)-
CC ginsenoside C-K + H(+) + UDP; Xref=Rhea:RHEA:57976,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:75950, ChEBI:CHEBI:77146; EC=2.4.1.363;
CC Evidence={ECO:0000269|PubMed:24603359, ECO:0000269|PubMed:26032089};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57977;
CC Evidence={ECO:0000269|PubMed:24603359, ECO:0000269|PubMed:26032089};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(20S)-ginsenoside Rg3 + UDP-alpha-D-glucose = (20S)-
CC ginsenoside Rd + H(+) + UDP; Xref=Rhea:RHEA:57984, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:67988,
CC ChEBI:CHEBI:67991; EC=2.4.1.363;
CC Evidence={ECO:0000269|PubMed:26032089};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57985;
CC Evidence={ECO:0000269|PubMed:26032089};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(20S)-ginsenoside Rh2 + UDP-alpha-D-glucose = (20S)-
CC ginsenoside F2 + H(+) + UDP; Xref=Rhea:RHEA:57988, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:77145,
CC ChEBI:CHEBI:77147; EC=2.4.1.363;
CC Evidence={ECO:0000269|PubMed:26032089};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57989;
CC Evidence={ECO:0000269|PubMed:26032089};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(20S)-protopanaxatriol + UDP-alpha-D-glucose = (20S)-
CC ginsenoside F1 + H(+) + UDP; Xref=Rhea:RHEA:57980, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:75951,
CC ChEBI:CHEBI:77150; EC=2.4.1.363;
CC Evidence={ECO:0000269|PubMed:26032089};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57981;
CC Evidence={ECO:0000269|PubMed:26032089};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dammarenediol-II + UDP-alpha-D-glucose = (20S)-20-O-(beta-D-
CC glucosyl)-3-hydroxydammarene + H(+) + UDP; Xref=Rhea:RHEA:59044,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:62416, ChEBI:CHEBI:142484; EC=2.4.1.363;
CC Evidence={ECO:0000269|PubMed:24603359, ECO:0000269|PubMed:26032089};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59045;
CC Evidence={ECO:0000269|PubMed:24603359, ECO:0000269|PubMed:26032089};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=359 uM for protopanaxadiol {ECO:0000269|PubMed:24603359};
CC KM=178 uM for dammarenediol II {ECO:0000269|PubMed:24603359};
CC Vmax=254 nmol/min/mg enzyme with protopanaxadiol as substrate
CC {ECO:0000269|PubMed:24603359};
CC Vmax=423 nmol/min/mg enzyme with dammarenediol II as substrate
CC {ECO:0000269|PubMed:24603359};
CC Note=kcat is 9.4 sec(-1) with protopanaxadiol as substrate
CC (PubMed:24603359). kcat is 12.7 sec(-1) with dammarenediol II as
CC substrate (PubMed:24603359). {ECO:0000269|PubMed:24603359};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in leaves and flowers, and, to a
CC lower extent, in roots and stems. {ECO:0000269|PubMed:24603359}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; KF377585; AIE12479.1; -; mRNA.
DR AlphaFoldDB; A0A068J840; -.
DR SMR; A0A068J840; -.
DR KEGG; ag:AIE12479; -.
DR UniPathway; UPA00213; -.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Isoprene biosynthesis; Transferase.
FT CHAIN 1..475
FT /note="UDP-glycosyltransferase 1"
FT /id="PRO_0000446969"
FT BINDING 278
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 344..345
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 362..370
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 384..387
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT SITE 144
FT /note="Essential for the glycosylation activity toward the
FT C20-OH of protopanaxatriol (PPT)"
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 10
FT /note="A->V: Reduced ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 13
FT /note="I->F: Reduced ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 38
FT /note="L->F: Normal ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 40
FT /note="A->S: Normal ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 82
FT /note="H->C: Normal ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT) and gained ability
FT to transfer a glucose residue to the C6-OH; thus producing
FT both ginsenosides F1 and Rh1."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 85
FT /note="F->L: Normal ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 134
FT /note="T->I: Normal ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 144
FT /note="H->F: Normal ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT) and gained weak
FT ability to transfer a glucose residue to the C6-OH; thus
FT producing both ginsenosides F1 and Rh1."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 144
FT /note="H->Y: Loss of glycosylase activity toward
FT protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
FT MUTAGEN 388
FT /note="Q->H: Normal ability to transfer a glucose residue
FT to the C20-OH of protopanaxatriol (PPT)."
FT /evidence="ECO:0000269|PubMed:26032089"
SQ SEQUENCE 475 AA; 53374 MW; A95B7ED4AF198880 CRC64;
MKSELIFLPA PAIGHLVGMV EMAKLFISRH ENLSVTVLIA KFYMDTGVDN YNKSLLTNPT
PRLTIVNLPE TDPQNYMLKP RHAIFPSVIE TQKTHVRDII SGMTQSESTQ VVGLLADLLF
INIMDIANEF NVPTYVYSPA GAGHLGLAFH LQTLNDKKQD VTEFRNSDTE LLVPSFANPV
PAEVLPSMYV DKEGGYDYLF SLFRRCRESK AIIINTFEEL EPYAINSLRM DSMIPPIYPV
GPILNLNGDG QNSDEAAVIL GWLDDQPPSS VVFLCFGSYG SFQENQVKEI AMGLERSGHR
FLWSLRPSIP KGETKLQLKY SNLKEILPVG FLDRTSCVGK VIGWAPQVAV LGHESVGGFL
SHCGWNSTLE SVWCGVPVAT WPMYGEQQLN AFEMVKELGI AVEIEVDYKK DYFNMKNDFI
VRAEEIETKI KKLMMDENNS EIRKKVKEMK EKSRAAMSEN GSSYNSLAKL FEEIM
