UGT29_PANGI
ID UGT29_PANGI Reviewed; 442 AA.
AC A0A0A6ZFY4; A0A0D5ZDH7;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 04-MAR-2015, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=UDP-glucosyltransferase 29 {ECO:0000303|PubMed:25769286};
DE Short=UGTPg29 {ECO:0000303|PubMed:25769286, ECO:0000303|PubMed:29509695};
DE EC=2.4.1.365 {ECO:0000269|PubMed:25320211, ECO:0000269|PubMed:25769286};
DE AltName: Full=UDP-glucosyltransferase 94B1 {ECO:0000303|PubMed:25320211};
DE Short=PgUGT94B1 {ECO:0000303|PubMed:25320211};
DE AltName: Full=UDP-glucosyltransferase 94Q2 {ECO:0000303|PubMed:27746309};
DE Short=PgUGT94Q2 {ECO:0000303|PubMed:29509695};
GN Name=UGT29 {ECO:0000303|PubMed:25769286};
GN Synonyms=UGT94B1 {ECO:0000303|PubMed:25320211},
GN UGT94Q2 {ECO:0000303|PubMed:27746309};
OS Panax ginseng (Korean ginseng).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Araliaceae; Panax.
OX NCBI_TaxID=4054;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, AND INDUCTION BY METHYL
RP JASMONATE.
RX PubMed=25320211; DOI=10.1093/pcp/pcu147;
RA Jung S.-C., Kim W., Park S.C., Jeong J., Park M.K., Lim S., Lee Y.,
RA Im W.-T., Lee J.H., Choi G., Kim S.C.;
RT "Two ginseng UDP-glycosyltransferases synthesize ginsenoside Rg3 and Rd.";
RL Plant Cell Physiol. 55:2177-2188(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=25769286; DOI=10.1016/j.ymben.2015.03.003;
RA Wang P., Wei Y., Fan Y., Liu Q., Wei W., Yang C., Zhang L., Zhao G.,
RA Yue J., Yan X., Zhou Z.;
RT "Production of bioactive ginsenosides Rh2 and Rg3 by metabolically
RT engineered yeasts.";
RL Metab. Eng. 29:97-105(2015).
RN [3]
RP FUNCTION, AND INDUCTION BY ASPERGILLUS NIGER.
RX PubMed=27746309; DOI=10.1016/j.jbiotec.2016.10.011;
RA Li J., Liu S., Wang J., Li J., Liu D., Li J., Gao W.;
RT "Fungal elicitors enhance ginsenosides biosynthesis, expression of
RT functional genes as well as signal molecules accumulation in adventitious
RT roots of Panax ginseng C. A. Mey.";
RL J. Biotechnol. 239:106-114(2016).
RN [4]
RP REVIEW.
RX PubMed=29378087; DOI=10.1002/bab.1649;
RA Lu J., Li J., Wang S., Yao L., Liang W., Wang J., Gao W.;
RT "Advances in ginsenoside biosynthesis and metabolic regulation.";
RL Biotechnol. Appl. Biochem. 65:514-522(2018).
RN [5]
RP REVIEW, AND NOMENCLATURE.
RX PubMed=29509695; DOI=10.3390/molecules23030589;
RA Yang J.-L., Hu Z.-F., Zhang T.-T., Gu A.-D., Gong T., Zhu P.;
RT "Progress on the studies of the key enzymes of ginsenoside biosynthesis.";
RL Molecules 23:0-0(2018).
CC -!- FUNCTION: Component of the dammarane-type triterpene saponins (e.g.
CC PPD-type ginsenosides or panaxosides) biosynthetic pathway
CC (PubMed:25320211, PubMed:27746309, PubMed:29378087, PubMed:25769286).
CC Glycosyltransferase that catalyzes the conversion of ginsenoside Rh2 to
CC ginsenoside Rg3 (PubMed:25320211, PubMed:27746309, PubMed:25769286).
CC Triggers the biosynthesis of ginsenoside Rd from ginsenoside F2
CC (PubMed:25320211). {ECO:0000269|PubMed:25320211,
CC ECO:0000269|PubMed:25769286, ECO:0000269|PubMed:27746309,
CC ECO:0000303|PubMed:29378087}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(20S)-ginsenoside F2 + UDP-alpha-D-glucose = (20S)-ginsenoside
CC Rd + H(+) + UDP; Xref=Rhea:RHEA:58004, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:67988,
CC ChEBI:CHEBI:77145; EC=2.4.1.365;
CC Evidence={ECO:0000269|PubMed:25320211};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:58005;
CC Evidence={ECO:0000269|PubMed:25320211};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(20S)-ginsenoside Rh2 + UDP-alpha-D-glucose = (20S)-
CC ginsenoside Rg3 + H(+) + UDP; Xref=Rhea:RHEA:58000,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:67991, ChEBI:CHEBI:77147; EC=2.4.1.365;
CC Evidence={ECO:0000269|PubMed:25320211, ECO:0000269|PubMed:25769286};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:58001;
CC Evidence={ECO:0000269|PubMed:25320211, ECO:0000269|PubMed:25769286};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=149 uM for ginsenoside Rh2 (at pH 8 and 40 degrees Celsius)
CC {ECO:0000269|PubMed:25769286};
CC KM=311 uM for ginsenoside Rh2 (at pH 7 and 35 degrees Celsius)
CC {ECO:0000269|PubMed:25320211};
CC KM=188 uM for ginsenoside F2 (at pH 7 and 35 degrees Celsius)
CC {ECO:0000269|PubMed:25320211};
CC Vmax=25 nmol/min/mg enzyme with ginsenoside Rh2 as substrate (at pH 8
CC and 40 degrees Celsius) {ECO:0000269|PubMed:25769286};
CC Note=kcat is 8.6 sec(-1) with ginsenoside Rh2 as substrate (at pH 7
CC and 35 degrees Celsius). kcat is 0.41 sec(-1) with ginsenoside F2 as
CC substrate (at pH 7 and 35 degrees Celsius) (PubMed:25320211). kcat is
CC 1111 sec(-1) with ginsenoside Rh2 as substrate (at pH 8 and 40
CC degrees Celsius) (PubMed:25769286). {ECO:0000269|PubMed:25320211,
CC ECO:0000269|PubMed:25769286};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at higher levels in roots than in leaves.
CC {ECO:0000269|PubMed:25320211}.
CC -!- INDUCTION: Induced by methyl jasmonate (MeJA) in roots and leaves
CC (PubMed:25320211). Induced by A.niger mycelium-derived elicitor, thus
CC improving ginsenosides production in adventitious roots culture
CC (PubMed:27746309). {ECO:0000269|PubMed:25320211,
CC ECO:0000269|PubMed:27746309}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; JX898530; AGR44632.1; -; Genomic_DNA.
DR EMBL; KM401911; AKA44579.1; -; mRNA.
DR AlphaFoldDB; A0A0A6ZFY4; -.
DR SMR; A0A0A6ZFY4; -.
DR KEGG; ag:AGR44632; -.
DR BioCyc; MetaCyc:MON-20534; -.
DR BRENDA; 2.4.1.365; 7895.
DR UniPathway; UPA00213; -.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0002238; P:response to molecule of fungal origin; IEP:UniProtKB.
DR GO; GO:0016135; P:saponin biosynthetic process; IDA:UniProtKB.
DR GO; GO:0046246; P:terpene biosynthetic process; IDA:UniProtKB.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Isoprene biosynthesis; Transferase.
FT CHAIN 1..442
FT /note="UDP-glucosyltransferase 29"
FT /id="PRO_0000446962"
FT BINDING 261
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 317..318
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 335..343
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 357..360
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT CONFLICT 5
FT /note="N -> K (in Ref. 2; AKA44579)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 442 AA; 49133 MW; 9B4D41867D26672E CRC64;
MDNQNGRISI ALLPFLAHGH ISPFFELAKQ LAKRNCNVFL CSTPINLSSI KDKDSSASIK
LVELHLPSSP DLPPHYHTTN GLPSHLMLPL RNAFETAGPT FSEILKTLNP DLLIYDFNPS
WAPEIASSHN IPAVYFLTTA AASSSIGLHA FKNPGEKYPF PDFYDNSNIT PEPPSADNMK
LLHDFIACFE RSCDIILIKS FRELEGKYID LLSTLSDKTL VPVGPLVQDP MGHNEDPKTE
QIINWLDKRA ESTVVFVCFG SEYFLSNEEL EEVAIGLEIS TVNFIWAVRL IEGEKKGILP
EGFVQRVGDR GLVVEGWAPQ ARILGHSSTG GFVSHCGWSS IAESMKFGVP VIAMARHLDQ
PLNGKLAAEV GVGMEVVRDE NGKYKREGIA EVIRKVVVEK SGEVIRRKAR ELSEKMKEKG
EQEIDRALEE LVQICKKKKD EQ
