ID   UGT4_DACCO              Reviewed;         504 AA.
AC   A0A291PQG3;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=UDP-glycosyltransferase UGT4 {ECO:0000305};
DE            EC=2.4.1.- {ECO:0000305};
DE   AltName: Full=UDP-glucosyltransferase 4 {ECO:0000303|PubMed:29215010};
DE            Short=DcUGT4 {ECO:0000303|PubMed:29215010};
DE   Flags: Precursor;
GN   Name=UGT4 {ECO:0000303|PubMed:29215010};
OS   Dactylopius coccus (Cochineal).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Coccoidea;
OC   Dactylopiidae; Dactylopius.
OX   NCBI_TaxID=765876 {ECO:0000312|EMBL:ATL15305.1};
RN   [1] {ECO:0000312|EMBL:ATL15305.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY,
RP   SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX   PubMed=29215010; DOI=10.1038/s41467-017-02031-z;
RA   Kannangara R., Siukstaite L., Borch-Jensen J., Madsen B., Kongstad K.T.,
RA   Staerk D., Bennedsen M., Okkels F.T., Rasmussen S.A., Larsen T.O.,
RA   Frandsen R.J.N., Moeller B.L.;
RT   "Characterization of a membrane-bound C-glucosyltransferase responsible for
RT   carminic acid biosynthesis in Dactylopius coccus Costa.";
RL   Nat. Commun. 8:1987-1987(2017).
CC   -!- FUNCTION: Catalyzes the transfer of a glycosyl group from a UDP-sugar
CC       to an acceptor molecule. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000269|PubMed:29215010};
CC       Single-pass type I membrane protein {ECO:0000305|PubMed:29215010}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in adult female.
CC       {ECO:0000269|PubMed:29215010}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000255|RuleBase:RU003718}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KY860726; ATL15305.1; -; mRNA.
DR   AlphaFoldDB; A0A291PQG3; -.
DR   SMR; A0A291PQG3; -.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Membrane;
KW   Microsome; Signal; Transferase; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..504
FT                   /note="UDP-glycosyltransferase UGT4"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5011814698"
FT   TOPO_DOM        24..474
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        475..495
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        496..504
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   CARBOHYD        54
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        66
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        69
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        422
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   504 AA;  58644 MW;  645B014719B3F72E CRC64;
     MTLLRDLLLL YINSLLFINP SIGENILVFL PTKTYSHFKP LEPLFQELAM RGHNVTVFSG
     FSLTKNISNY SSIVFSAEIE FVNIGMGNLR KQSRIYNWIY VHNELQNYFT QLISDNQLQE
     LLSNKDTQFD LIFIELYHVD GVFALSHRFN CPIIGLSFQP VLPIYNWLIG NPTTFSYIPH
     VYLPFTDIMS FWKRIINAVF SIFTAAFYNF VSTKGYQKHV DLLLRQTESP KLNIEELSES
     LSLILAEFHF SSAYTRPNLP NVIDIAGIHI QSPKPLPQDL LDFLDQSEHG VIYVSLGTLI
     DPIHTDHLGL NLINVFRKLR QRVIWKWKKE FFHDVPKNVL IGEWFPQIDI LNHPRCKLFI
     SHGGYHSMLE SIYSSVPILG IPFFTDQHHN TAIIEKLKIG KKASTEASEE DLLTAVKELL
     SNETFKRNSQ HQSSIFRDRP MSPMDTAIYW TEYILRYKGA SHMKSAVIDL YWFQYILLDI
     ILFYSLIVLI LLCILRIFFR MLTK