ID   UGT6_CATRO              Reviewed;         487 AA.
AC   F8WLS6;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=7-deoxyloganetin glucosyltransferase;
DE            EC=2.4.1.324;
DE   AltName: Full=Iridoid 1-O-glucosyltransferase;
DE   AltName: Full=UDP-glucose glucosyltransferase 6;
DE            Short=CrUGT6;
DE   AltName: Full=UDP-glycosyltransferase 85A23;
GN   Name=UGT85A23; Synonyms=UGT6;
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=21799001; DOI=10.1074/jbc.m111.242586;
RA   Nagatoshi M., Terasaka K., Nagatsu A., Mizukami H.;
RT   "Iridoid-specific Glucosyltransferase from Gardenia jasminoides.";
RL   J. Biol. Chem. 286:32866-32874(2011).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=24104568; DOI=10.1105/tpc.113.115154;
RA   Asada K., Salim V., Masada-Atsumi S., Edmunds E., Nagatoshi M.,
RA   Terasaka K., Mizukami H., De Luca V.;
RT   "A 7-deoxyloganetic Acid glucosyltransferase contributes a key step in
RT   secologanin biosynthesis in madagascar periwinkle.";
RL   Plant Cell 25:4123-4134(2013).
CC   -!- FUNCTION: Iridoid glucosyltransferase acting exclusively on 7-
CC       deoxyloganetin. No activity with 7-deoxyloganetic acid.
CC       {ECO:0000269|PubMed:24104568}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-deoxyloganetin + UDP-alpha-D-glucose = 7-deoxyloganin + H(+)
CC         + UDP; Xref=Rhea:RHEA:39899, ChEBI:CHEBI:15378, ChEBI:CHEBI:18370,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:76849;
CC         EC=2.4.1.324; Evidence={ECO:0000269|PubMed:24104568};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.202 mM for 7-deoxyloganetin {ECO:0000269|PubMed:24104568};
CC         KM=0.117 mM for UPD-glucose {ECO:0000269|PubMed:24104568};
CC         Note=kcat is 0.0355 sec(-1) for 7-deoxyloganetin. kcat is 0.0320
CC         sec(-1) for UPD-glucose.;
CC   -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:24104568}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB591741; BAK55749.1; -; mRNA.
DR   AlphaFoldDB; F8WLS6; -.
DR   SMR; F8WLS6; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   KEGG; ag:BAK55749; -.
DR   BRENDA; 2.4.1.324; 1211.
DR   GO; GO:0035251; F:UDP-glucosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:1900994; P:(-)-secologanin biosynthetic process; IDA:UniProtKB.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF00201; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Transferase.
FT   CHAIN           1..487
FT                   /note="7-deoxyloganetin glucosyltransferase"
FT                   /id="PRO_0000430133"
FT   BINDING         307
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         364..366
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         381..389
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         403..406
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   487 AA;  54611 MW;  A6E423A362B9E0CF CRC64;
     MGSLSSSDYS KKPHAVCIPY PAQGHINPML KLAKLLHYKG FHITFVNTEF NHKRLLKSRG
     SDSLKGLHSF QFKTIPDGLP PSDVDATQDI PSLCESTTTH CLVPFKQLLQ KLNDTSSSEV
     PPVSCVVSDA VMSFTISAAQ ELDIPEVLFW TPSACGVLGY MHYAQLIDKG LTPLKDASYF
     SNGFLDQVLD WIPGMEGIRL RDLPTFLRTT NPDEYMIKFI LQETERSKKA SAIVLNTFQE
     LESEVIDSLS TLLPPIYPIG PLQILQNQVD DESLKVLGSN LWKEEPECLE WLDTKDPNSV
     VYVNFGSITV MTNDQLIEFA WGLANSKQNF LWIIRPDLIS GESSILGEEF VEETKERGLI
     ASWCHQEQVI NHPAIGGFLT HNGWNSTIES ISSGVPMICW PFFAEQQTNC RFCCNKWGIG
     MEINSDVKRD EVESLVKELM VGEKGKEMKK KALEWKNIAE VTTTKPDGSS YSNLEKLIKV
     LKSKPSH