ID   UGT8_CATRO              Reviewed;         482 AA.
AC   U3U992;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   11-DEC-2013, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=7-deoxyloganetic acid glucosyltransferase;
DE            EC=2.4.1.323;
DE   AltName: Full=UDP-glucose glucosyltransferase 8;
DE            Short=CrUGT8;
DE   AltName: Full=UDP-glycosyltransferase 709C2;
GN   Name=UGT709C2; Synonyms=UGT8;
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
RX   PubMed=24104568; DOI=10.1105/tpc.113.115154;
RA   Asada K., Salim V., Masada-Atsumi S., Edmunds E., Nagatoshi M.,
RA   Terasaka K., Mizukami H., De Luca V.;
RT   "A 7-deoxyloganetic Acid glucosyltransferase contributes a key step in
RT   secologanin biosynthesis in madagascar periwinkle.";
RL   Plant Cell 25:4123-4134(2013).
CC   -!- FUNCTION: Iridoid glucosyltransferase acting exclusively on 7-
CC       deoxyloganetic acid. No activity with 7-deoxyloganetin. Catalyzes the
CC       fourth to last step in secologanin biosynthesis.
CC       {ECO:0000269|PubMed:24104568}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-deoxyloganetate + UDP-alpha-D-glucose = 7-deoxyloganate +
CC         H(+) + UDP; Xref=Rhea:RHEA:39895, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:76844,
CC         ChEBI:CHEBI:76846; EC=2.4.1.323;
CC         Evidence={ECO:0000269|PubMed:24104568};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.088 mM for 7-deoxyloganetic acid {ECO:0000269|PubMed:24104568};
CC         KM=5.38 mM for UPD-glucose {ECO:0000269|PubMed:24104568};
CC         Note=kcat is 0.130 sec(-1) for 7-deoxyloganetic acid. kcat is 0.325
CC         sec(-1) for UPD-glucose.;
CC   -!- TISSUE SPECIFICITY: Expressed in leaves, roots and stems. Lower levels
CC       of expression in flowers. Preferentially expressed in internal phloem
CC       parenchyma cells. {ECO:0000269|PubMed:24104568}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AB733667; BAO01109.1; -; mRNA.
DR   AlphaFoldDB; U3U992; -.
DR   SMR; U3U992; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   KEGG; ag:BAO01109; -.
DR   BioCyc; MetaCyc:MON-18444; -.
DR   BRENDA; 2.4.1.323; 1211.
DR   GO; GO:0102970; F:7-deoxyloganetic acid glucosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0035251; F:UDP-glucosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:1900994; P:(-)-secologanin biosynthetic process; IDA:UniProtKB.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..482
FT                   /note="7-deoxyloganetic acid glucosyltransferase"
FT                   /id="PRO_0000430135"
FT   BINDING         303
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         379..387
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
FT   BINDING         401..404
FT                   /ligand="UDP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:58885"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   482 AA;  54245 MW;  D0839ECA8E33C87D CRC64;
     MGSQETNLAP HVLIFPLPIQ GHVNSMLRLA ELLCLAELDI TFIVSEFSHS RLIKHTNVAS
     RFARYPGFQF QPISDGLPDD HPRAGERVMD ILPSTKNVTG PLFKQMMVEN KCFSSATRRP
     ITCIIADGVL SFAGDFAQEK GIPLIYFRTV SACSFWACFC MPELIESGDI PIKGNGMDLI
     VKSVPGMETF LRRRDLPGFC RVNDINEPKL QILKTETRQT TRAQAAILNT FEDLEGPILS
     QIRKHMPRLF TIGPSHSHLT SRLETKNIKT LISSGSFWEE DRSCVDWLDA QPPRSVLYVS
     FGSITVVTRD QLLEFWYGLV NSGQRFLWVM RPDSIMGKDG QSQIPADLEE GTKARGYMVG
     WAPQEEVLNH PAIGGFLTHS GWNSTLESIV AGVPMICWPY FADQMINSRF VSEIWKIGLD
     MKDTCDRETI VKMVRELMEI RKDEFLQRAD HMAKLAKEAV SEGGSSYSNL DGLVDYIKSL
     II