UGT9_GARJA
ID UGT9_GARJA Reviewed; 444 AA.
AC F8WKW8;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=Beta-D-glucosyl crocetin beta-1,6-glucosyltransferase;
DE EC=2.4.1.330 {ECO:0000269|PubMed:22569263};
DE AltName: Full=UDP-glucose glucosyltransferase 9;
DE Short=GjUGT9;
DE AltName: Full=UDP-glycosyltransferase 94E5;
GN Name=UGT94E5; Synonyms=UGT9;
OS Gardenia jasminoides (Cape jasmine) (Gardenia augusta).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex;
OC Gardenieae - Pavetteae clade; Gardenieae; Gardenia.
OX NCBI_TaxID=114476;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=21799001; DOI=10.1074/jbc.m111.242586;
RA Nagatoshi M., Terasaka K., Nagatsu A., Mizukami H.;
RT "Iridoid-specific Glucosyltransferase from Gardenia jasminoides.";
RL J. Biol. Chem. 286:32866-32874(2011).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP SPECIFICITY.
RX PubMed=22569263; DOI=10.1016/j.febslet.2012.03.003;
RA Nagatoshi M., Terasaka K., Owaki M., Sota M., Inukai T., Nagatsu A.,
RA Mizukami H.;
RT "UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin
RT in Gardenia jasminoides.";
RL FEBS Lett. 586:1055-1061(2012).
CC -!- FUNCTION: Glucosyltransferase catalyzing the beta 1-6 glucosylation of
CC the sugar moiety of crocetin glucosyl esters to produce crocetin
CC gentiobiosyl esters. Weak activity toward curcumin glucosides, but no
CC activity with flavonoid glucosides, coumarin glucosides, 4-nitrophenyl
CC glucoside or crocetin. Involved with UGT75L6 in sequential
CC glycosylation of crocetin to crocin (bis(beta-D-gentiobiosyl)
CC crocetin). {ECO:0000269|PubMed:22569263}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-glucosyl crocetin + UDP-alpha-D-glucose = beta-D-
CC gentiobiosyl crocetin + H(+) + UDP; Xref=Rhea:RHEA:42388,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:62766, ChEBI:CHEBI:62770; EC=2.4.1.330;
CC Evidence={ECO:0000269|PubMed:22569263};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=bis(beta-D-glucosyl) crocetin + UDP-alpha-D-glucose = beta-D-
CC gentiobiosyl beta-D-glucosyl crocetin + H(+) + UDP;
CC Xref=Rhea:RHEA:30899, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:62768, ChEBI:CHEBI:62771;
CC EC=2.4.1.330; Evidence={ECO:0000269|PubMed:22569263};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-gentiobiosyl beta-D-glucosyl crocetin + UDP-alpha-D-
CC glucose = bis(beta-D-gentiobiosyl) crocetin + H(+) + UDP;
CC Xref=Rhea:RHEA:38859, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:62771, ChEBI:CHEBI:79068;
CC EC=2.4.1.330; Evidence={ECO:0000269|PubMed:22569263};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.072 mM for beta-D-glucosyl crocetin
CC {ECO:0000269|PubMed:22569263};
CC KM=0.023 mM for bis(beta-D-glucosyl) crocetin
CC {ECO:0000269|PubMed:22569263};
CC Note=kcat is 0.036 sec(-1) for beta-D-glucosyl crocetin. kcat is
CC 0.071 sec(-1) for bis(beta-D-glucosyl) crocetin.;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:22569263}.
CC -!- MISCELLANEOUS: Crocin is a yellow pigment that accumulates during fruit
CC development. No other crocetin glucosyl esters detected in neither
CC flowers, leaves, nor stems. No correlation between mRNA expression and
CC pigment accumulation (PubMed:22569263). {ECO:0000305|PubMed:22569263}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB555739; BAK55744.1; -; mRNA.
DR AlphaFoldDB; F8WKW8; -.
DR SMR; F8WKW8; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR KEGG; ag:BAK55744; -.
DR BioCyc; MetaCyc:MON-18780; -.
DR BRENDA; 2.4.1.330; 2400.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProt.
DR GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..444
FT /note="Beta-D-glucosyl crocetin beta-1,6-
FT glucosyltransferase"
FT /id="PRO_0000430138"
FT BINDING 336..344
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 358..361
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 444 AA; 49798 MW; A8642683096DE510 CRC64;
MFPWLAYGHI SPYLELAKRL TDRGFAIYIC STPINLGFIK KRITGKYSVT IKLVELHLPD
TPELPPHYHT TNGLPPHLMA TLKRALNGAK PELSNILKTL KPDFVIYDAT QTWTAALTVA
HNIPAVKFLT SSVSMLAYFC HLFMKPGIEF PFPAIYLSDF EQAKARTAAQ DARADAEEND
PAAERPNRDC DSIFLVKSSR AIEGKYIDYL FDLMKLKMLP VGMLVEEPVK DDQGDNSNEL
IQWLGTKSQR STVLVSFGTE YFLTKEEMEE IAHGLELSEV NFIWVVRFAM GQKIRPDEAL
PEGFLERVGD RGRIVEGWAP QSEVLAHPST GGFICHCGWN SVVESIEFGV PVIAMPMHLD
QPLNARLVVE IGAGMEVVRD ETGKFDRKEI ARAIKDAMVE KTGENTRAKM LDVKGRVELK
EKQELDEVAE LLTQLVTETT QSSN
