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UHPA_ECOLI
ID   UHPA_ECOLI              Reviewed;         196 AA.
AC   P0AGA6; P10940; Q2M7Y2;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Transcriptional regulatory protein UhpA {ECO:0000305};
GN   Name=uhpA; OrderedLocusNames=b3669, JW3644;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3301805; DOI=10.1128/jb.169.8.3556-3563.1987;
RA   Friedrich M.J., Kadner R.J.;
RT   "Nucleotide sequence of the uhp region of Escherichia coli.";
RL   J. Bacteriol. 169:3556-3563(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1569007; DOI=10.1128/jb.174.9.2754-2762.1992;
RA   Island M.D., Wei B.-Y., Kadner R.J.;
RT   "Structure and function of the uhp genes for the sugar phosphate transport
RT   system in Escherichia coli and Salmonella typhimurium.";
RL   J. Bacteriol. 174:2754-2762(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA   Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT   "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT   organizational symmetry around the origin of replication.";
RL   Genomics 16:551-561(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   PROTEIN SEQUENCE OF 1-20, FUNCTION, ACTIVITY REGULATION, PHOSPHORYLATION AT
RP   ASP-54, AND MUTAGENESIS OF ASP-54.
RX   PubMed=8999880; DOI=10.1074/jbc.272.3.1910;
RA   Dahl J.L., Wei B.Y., Kadner R.J.;
RT   "Protein phosphorylation affects binding of the Escherichia coli
RT   transcription activator UhpA to the uhpT promoter.";
RL   J. Biol. Chem. 272:1910-1919(1997).
RN   [7]
RP   FUNCTION.
RX   PubMed=3038843; DOI=10.1128/jb.169.8.3546-3555.1987;
RA   Weston L.A., Kadner R.J.;
RT   "Identification of uhp polypeptides and evidence for their role in
RT   exogenous induction of the sugar phosphate transport system of Escherichia
RT   coli K-12.";
RL   J. Bacteriol. 169:3546-3555(1987).
RN   [8]
RP   IDENTIFICATION BY 2D-GEL.
RX   PubMed=9298644; DOI=10.1002/elps.1150180805;
RA   VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT   "Escherichia coli proteome analysis using the gene-protein database.";
RL   Electrophoresis 18:1243-1251(1997).
RN   [9]
RP   PHOSPHORYLATION BY UHPB.
RX   PubMed=11053370; DOI=10.1128/jb.182.22.6279-6286.2000;
RA   Wright J.S., Olekhnovich I.N., Touchie G., Kadner R.J.;
RT   "The histidine kinase domain of UhpB inhibits UhpA action at the
RT   Escherichia coli uhpT promoter.";
RL   J. Bacteriol. 182:6279-6286(2000).
RN   [10]
RP   PHOSPHORYLATION AND DEPHOSPHORYLATION BY UHPB.
RX   PubMed=11739766; DOI=10.1099/00221287-147-12-3345;
RA   Verhamme D.T., Arents J.C., Postma P.W., Crielaard W., Hellingwerf K.J.;
RT   "Glucose-6-phosphate-dependent phosphoryl flow through the Uhp two-
RT   component regulatory system.";
RL   Microbiology 147:3345-3352(2001).
CC   -!- FUNCTION: Part of the UhpABC signaling cascade that controls the
CC       expression of the hexose phosphate transporter UhpT. Activates the
CC       transcription of the uhpT gene. Acts by binding specifically to the
CC       uhpT promoter region. {ECO:0000269|PubMed:3038843,
CC       ECO:0000269|PubMed:8999880}.
CC   -!- ACTIVITY REGULATION: Phosphorylation by UhpB enhances DNA binding
CC       activity. {ECO:0000269|PubMed:8999880}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated and dephosphorylated by UhpB.
CC       {ECO:0000269|PubMed:11053370, ECO:0000269|PubMed:11739766}.
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DR   EMBL; M17102; AAA24720.1; -; Genomic_DNA.
DR   EMBL; M89479; AAA24724.1; -; Genomic_DNA.
DR   EMBL; L10328; AAA62021.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76692.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77624.1; -; Genomic_DNA.
DR   PIR; A26925; BVECAU.
DR   RefSeq; NP_418125.1; NC_000913.3.
DR   RefSeq; WP_000633668.1; NZ_STEB01000015.1.
DR   AlphaFoldDB; P0AGA6; -.
DR   SMR; P0AGA6; -.
DR   BioGRID; 4262584; 23.
DR   BioGRID; 852484; 3.
DR   DIP; DIP-11082N; -.
DR   IntAct; P0AGA6; 4.
DR   STRING; 511145.b3669; -.
DR   jPOST; P0AGA6; -.
DR   PaxDb; P0AGA6; -.
DR   PRIDE; P0AGA6; -.
DR   EnsemblBacteria; AAC76692; AAC76692; b3669.
DR   EnsemblBacteria; BAE77624; BAE77624; BAE77624.
DR   GeneID; 66672434; -.
DR   GeneID; 948178; -.
DR   KEGG; ecj:JW3644; -.
DR   KEGG; eco:b3669; -.
DR   PATRIC; fig|1411691.4.peg.3036; -.
DR   EchoBASE; EB1044; -.
DR   eggNOG; COG2197; Bacteria.
DR   HOGENOM; CLU_000445_90_1_6; -.
DR   InParanoid; P0AGA6; -.
DR   OMA; HPMWRDG; -.
DR   PhylomeDB; P0AGA6; -.
DR   BioCyc; EcoCyc:UHPA-MON; -.
DR   PRO; PR:P0AGA6; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   Pfam; PF00196; GerE; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; Direct protein sequencing; DNA-binding;
KW   Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..196
FT                   /note="Transcriptional regulatory protein UhpA"
FT                   /id="PRO_0000081257"
FT   DOMAIN          3..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          131..196
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        155..174
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   MOD_RES         54
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT                   ECO:0000269|PubMed:8999880"
FT   MUTAGEN         54
FT                   /note="D->N: Lack of phosphorylation."
FT                   /evidence="ECO:0000269|PubMed:8999880"
SQ   SEQUENCE   196 AA;  20889 MW;  8F409F8AADE08276 CRC64;
     MITVALIDDH LIVRSGFAQL LGLEPDLQVV AEFGSGREAL AGLPGRGVQV CICDISMPDI
     SGLELLSQLP KGMATIMLSV HDSPALVEQA LNAGARGFLS KRCSPDELIA AVHTVATGGC
     YLTPDIAIKL ASGRQDPLTK RERQVAEKLA QGMAVKEIAA ELGLSPKTVH VHRANLMEKL
     GVSNDVELAR RMFDGW
 
 
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