UIDB2_ECOLI
ID UIDB2_ECOLI Reviewed; 457 AA.
AC P0CE45; P30868; P77457;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 1.
DT 25-MAY-2022, entry version 43.
DE RecName: Full=Glucuronide carrier protein;
DE AltName: Full=Glucuronide permease;
GN Name=uidB; Synonyms=gusB, uidP;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Liang W.J., Jefferson R.A.;
RT "The gusB gene of Escherichia coli.";
RL Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
RX PubMed=3534890; DOI=10.1073/pnas.83.22.8447;
RA Jefferson R.A., Burgess S.M., Hirsh D.;
RT "Beta-glucuronidase from Escherichia coli as a gene-fusion marker.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:8447-8451(1986).
RN [3]
RP PROTEIN SEQUENCE OF 1-18, CHARACTERIZATION, INDUCTION, LACK OF TRANSPORT IN
RP K12, AND SUBCELLULAR LOCATION.
RC STRAIN=CE1;
RX PubMed=15774881; DOI=10.1128/jb.187.7.2377-2385.2005;
RA Liang W.-J., Wilson K.J., Xie H., Knol J., Suzuki S., Rutherford N.G.,
RA Henderson P.J.F., Jefferson R.A.;
RT "The gusBC genes of Escherichia coli encode a glucuronide transport
RT system.";
RL J. Bacteriol. 187:2377-2385(2005).
CC -!- FUNCTION: Responsible for the transport of glucuronide into the cell
CC energized by the proton motive force (probably by symport). Import is
CC enhanced by UidC (GusC, AC Q47706).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15774881}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15774881}.
CC -!- INDUCTION: In the presence of glucuronides.
CC {ECO:0000269|PubMed:15774881}.
CC -!- MISCELLANEOUS: In some K12 strains, the protein is inactive for
CC transport, probably due to a mutation at position 100.
CC -!- SIMILARITY: Belongs to the sodium:galactoside symporter (TC 2.A.2)
CC family. {ECO:0000305}.
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DR EMBL; M14641; AAA68924.1; -; Genomic_DNA.
DR RefSeq; WP_001075863.1; NZ_SSZK01000001.1.
DR AlphaFoldDB; P0CE45; -.
DR SMR; P0CE45; -.
DR TCDB; 2.A.2.3.1; the glycoside-pentoside-hexuronide (gph):cation symporter family.
DR PRIDE; P0CE45; -.
DR PhylomeDB; P0CE45; -.
DR PRO; PR:P0CE45; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR039672; MFS_2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001927; Na/Gal_symport.
DR InterPro; IPR018043; Na/Gal_symport_CS.
DR PANTHER; PTHR11328; PTHR11328; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00792; gph; 1.
DR PROSITE; PS00872; NA_GALACTOSIDE_SYMP; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Direct protein sequencing; Membrane;
KW Sugar transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..457
FT /note="Glucuronide carrier protein"
FT /id="PRO_0000392205"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..37
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..79
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..108
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..180
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..263
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..296
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..320
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..372
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..408
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 430..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 457 AA; 49892 MW; C47CCB538AC088C1 CRC64;
MNQQLSWRTI VGYSLGDVAN NFAFAMGALF LLSYYTDVAG VGAAAAGTML LLVRVFDAFA
DVFAGRVVDS VNTRWGKFRP FLLFGTAPLM IFSVLVFWVP TDWSHGSKVV YAYLTYMGLG
LCYSLVNIPY GSLATAMTQQ PQSRARLGAA RGIAASLTFV CLAFLIGPSI KNSSPEEMVS
VYHFWTIVLA IAGMVLYFIC FKSTRENVVR IVAQPSLNIS LQTLKRNRPL FMLCIGALCV
LISTFAVSAS SLFYVRYVLN DTGLFTVLVL VQNLVGTVAS APLVPGMVAR IGKKNTFLIG
ALLGTCGYLL FFWVSVWSLP VALVALAIAS IGQGVTMTVM WALEADTVEY GEYLTGVRIE
GLTYSLFSFT RKCGQAIGGS IPAFILGLSG YIANQVQTPE VIMGIRTSIA LVPCGFMLLA
FVIIWFYPLT DKKFKEIVVE IDNRKKVQQQ LISDITN
