ID   UL128_HCMVM             Reviewed;         171 AA.
AC   V9LLX6;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 1.
DT   29-SEP-2021, entry version 9.
DE   RecName: Full=Envelope protein UL128;
GN   Name=UL128;
OS   Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX   NCBI_TaxID=295027;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Davison A.J.;
RT   "Human cytomegalovirus RL11 gene family: variation, recombination and
RT   transcription.";
RL   Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND INTERACTION WITH GH; GL; UL130 AND UL131A.
RX   PubMed=17942555; DOI=10.1128/jvi.01910-07;
RA   Ryckman B.J., Rainish B.L., Chase M.C., Borton J.A., Nelson J.A.,
RA   Jarvis M.A., Johnson D.C.;
RT   "Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that
RT   mediates entry into epithelial and endothelial cells.";
RL   J. Virol. 82:60-70(2008).
RN   [3]
RP   FUNCTION.
RX   PubMed=21367908; DOI=10.1128/jvi.02100-10;
RA   Straschewski S., Patrone M., Walther P., Gallina A., Mertens T.,
RA   Frascaroli G.;
RT   "Protein pUL128 of human cytomegalovirus is necessary for monocyte
RT   infection and blocking of migration.";
RL   J. Virol. 85:5150-5158(2011).
RN   [4]
RP   FUNCTION.
RX   PubMed=23853586; DOI=10.1371/journal.ppat.1003463;
RA   Nogalski M.T., Chan G.C., Stevenson E.V., Collins-McMillen D.K.,
RA   Yurochko A.D.;
RT   "The HCMV gH/gL/UL128-131 complex triggers the specific cellular activation
RT   required for efficient viral internalization into target monocytes.";
RL   PLoS Pathog. 9:E1003463-E1003463(2013).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=23804643; DOI=10.1128/jvi.01167-13;
RA   Zhou M., Yu Q., Wechsler A., Ryckman B.J.;
RT   "Comparative analysis of gO isoforms reveals that strains of human
RT   cytomegalovirus differ in the ratio of gH/gL/gO and gH/gL/UL128-131 in the
RT   virion envelope.";
RL   J. Virol. 87:9680-9690(2013).
RN   [6]
RP   REVIEW.
RX   PubMed=24957713; DOI=10.4149/av_2014_02_103;
RA   Tao R., Xu J., Gao H., Zhao W., Shang S.;
RT   "Characteristics and functions of human cytomegalovirus UL128
RT   gene/protein.";
RL   Acta Virol. 58:103-107(2014).
RN   [7]
RP   FUNCTION, AND INTERACTION WITH HOST NRP2.
RX   PubMed=30057110; DOI=10.1016/j.cell.2018.06.028;
RA   Martinez-Martin N., Marcandalli J., Huang C.S., Arthur C.P., Perotti M.,
RA   Foglierini M., Ho H., Dosey A.M., Shriver S., Payandeh J., Leitner A.,
RA   Lanzavecchia A., Perez L., Ciferri C.;
RT   "An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a
RT   Central Viral Receptor.";
RL   Cell 0:0-0(2018).
CC   -!- FUNCTION: Plays a role in viral entry into host cells. Forms a
CC       pentameric complex at the surface of the viral envelope together with
CC       gH, gL, UL130 and UL131. This complex is required for entry in
CC       epithelial, endothelial and myeloid host cells (PubMed:23853586,
CC       PubMed:17942555). Mechanistically, engages host receptor(s) including
CC       neurophilin 2/NRP2 to mediate infection (PubMed:30057110).
CC       Additionally, monomeric UL128 may interfere with certain inflammatory
CC       cytokines to increase infection and dissemination by blocking monocytes
CC       migration (PubMed:21367908). {ECO:0000269|PubMed:17942555,
CC       ECO:0000269|PubMed:21367908, ECO:0000269|PubMed:23853586,
CC       ECO:0000269|PubMed:30057110}.
CC   -!- SUBUNIT: Forms the envelope pentamer complex (PC) composed of gH, gL,
CC       UL128, UL130, and UL131A (PubMed:17942555). The pentamer interacts with
CC       host NRP2 (PubMed:30057110). {ECO:0000269|PubMed:17942555,
CC       ECO:0000269|PubMed:30057110}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000269|PubMed:23804643}.
CC       Note=Found as a pentameric complex at the surface of virion envelope.
CC       {ECO:0000269|PubMed:23804643}.
CC   -!- SIMILARITY: Belongs to the HHV-5 UL128 protein family. {ECO:0000305}.
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DR   EMBL; JX512197; AFR54607.1; -; Genomic_DNA.
DR   SMR; V9LLX6; -.
DR   Proteomes; UP000169440; Genome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0098670; P:entry receptor-mediated virion attachment to host cell; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Membrane; Viral attachment to host cell;
KW   Viral attachment to host entry receptor; Viral envelope protein; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..171
FT                   /note="Envelope protein UL128"
FT                   /id="PRO_0000445468"
SQ   SEQUENCE   171 AA;  19773 MW;  9973D2EF8BF282ED CRC64;
     MSPKNLTPFL TALWLLLDHS RVPRVRAEEC CEFINVNHPP ERCYDFKMCN RFTVALRCPD
     GEVCYSPEKT AEIRGIVTTM THSLTRQVVH NKLTSCNYNP LYLEADGRIR CGKVNDKAQY
     LLGAAGSVPY RWINLEYDKI TRIVGLNQYL ESVKKHKRLD VCRAKMGYML Q