ID   UL20_SUHVN              Reviewed;         161 AA.
AC   Q00702;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   23-FEB-2022, entry version 56.
DE   RecName: Full=Protein UL20;
OS   Suid herpesvirus 1 (strain NIA-3) (SuHV-1) (Pseudorabies virus (strain
OS   NIA-3)).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10349;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1331512; DOI=10.1128/jvi.66.12.7096-7103.1992;
RA   Berns A.A., Kimman T.T., Pol J.J., Wagenaar F.F., de Wind N.N.;
RT   "The pseudorabies virus homology of the herpes simplex virus UL21 gene
RT   product is a capsid protein which is involved in capsid maturation.";
RL   J. Virol. 66:7096-7103(1992).
RN   [2]
RP   FUNCTION.
RX   PubMed=9188641; DOI=10.1128/jvi.71.7.5639-5646.1997;
RA   Fuchs W., Klupp B.G., Granzow H., Mettenleiter T.C.;
RT   "The UL20 gene product of pseudorabies virus functions in virus egress.";
RL   J. Virol. 71:5639-5646(1997).
RN   [3]
RP   FUNCTION IN GK PROCESSING.
RX   PubMed=10799582; DOI=10.1128/jvi.74.11.5083-5090.2000;
RA   Dietz P., Klupp B.G., Fuchs W., Kollner B., Weiland E., Mettenleiter T.C.;
RT   "Pseudorabies virus glycoprotein K requires the UL20 gene product for
RT   processing.";
RL   J. Virol. 74:5083-5090(2000).
CC   -!- FUNCTION: Plays an essential role in egress of virus particles from the
CC       nucleus, cytoplasmic envelopment and virus-induced cell fusion. Forms a
CC       functional protein complex with gK and this interaction is absolutely
CC       essential for their coordinate intracellular transport, gK
CC       glycosylation, expression on host cell surface, and function. Together,
CC       they modulate gB-mediated virus-induced cell fusion and virion egress
CC       and therefore actively participate in these processes (By similarity).
CC       {ECO:0000250, ECO:0000269|PubMed:10799582, ECO:0000269|PubMed:9188641}.
CC   -!- SUBUNIT: Interacts with gK (via N-terminus); this interaction plays a
CC       role in the coordinate transport of UL20 and gK to the trans-Golgi
CC       network (TGN), and is required for their cell surface expression.
CC       Interacts with gB (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cell membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Host endosome
CC       membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Host
CC       Golgi apparatus membrane {ECO:0000250}; Multi-pass membrane protein
CC       {ECO:0000250}. Host nucleus membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}. Note=During virion morphogenesis, this protein
CC       probably accumulates in the endosomes and trans-Golgi where secondary
CC       envelopment occurs. It is probably transported with gK to the cell
CC       surface from where it is endocytosed and directed to the trans-Golgi
CC       network (TGN) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae UL20 family.
CC       {ECO:0000305}.
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DR   EMBL; M95285; AAA47473.1; -; Genomic_DNA.
DR   GO; GO:0044175; C:host cell endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044200; C:host cell nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019058; P:viral life cycle; IEA:InterPro.
DR   InterPro; IPR007629; Herpes_UL20.
DR   Pfam; PF04544; Herpes_UL20; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host endosome; Host Golgi apparatus; Host membrane;
KW   Host nucleus; Membrane; Transmembrane; Transmembrane helix; Virion.
FT   CHAIN           1..161
FT                   /note="Protein UL20"
FT                   /id="PRO_0000115970"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   161 AA;  16641 MW;  218083FDEB74AA45 CRC64;
     MEDAAADVDA AADAKLTGEN DALLSSAFVG ARPPRPRFSS HVVSLLALAL ALRPACCLVL
     ALHGSRATLA ALLTALAFYA RAAVCAVLVA RNVARDRMPL SPAQQAALGL LAAARLAFLY
     VALDAGRHYA PALAGALYGA DCVCDALAFL LPRAYARSIM H