UL35_HCMVM
ID UL35_HCMVM Reviewed; 641 AA.
AC F5HE12;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 27.
DE RecName: Full=Tegument protein UL35;
GN Name=UL35;
OS Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX NCBI_TaxID=295027;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15105547; DOI=10.1099/vir.0.79888-0;
RA Dolan A., Cunningham C., Hector R.D., Hassan-Walker A.F., Lee L.,
RA Addison C., Dargan D.J., McGeoch D.J., Gatherer D., Emery V.C.,
RA Griffiths P.D., Sinzger C., McSharry B.P., Wilkinson G.W.G., Davison A.J.;
RT "Genetic content of wild-type human cytomegalovirus.";
RL J. Gen. Virol. 85:1301-1312(2004).
CC -!- FUNCTION: [Isoform UL35]: Plays important role in immediate-early gene
CC expression through interaction with UL82. Forms nuclear bodies in host
CC nucleus, independently of PML. In turn, UL35 nuclear bodies associate
CC with and remodel PML bodies. Through interaction with host DCAF1,
CC causes cells to accumulate in the G2 phase of the cell cycle by
CC inducing a DNA damage response. Regulates viral assembly by controlling
CC the localization of the essential gB through regulation of a retrograde
CC transport pathway. This modulation occurs via binding and inhibition of
CC host sorting nexin 5/SNX5. Also plays a role in the inhibition of
CC pattern recognition receptor-mediated type I interferon signaling at
CC the level of TBK1. {ECO:0000250|UniProtKB:P16766}.
CC -!- FUNCTION: [Isoform UL35A]: Promotes cytoplasmic UL82 accumulation and
CC inhibits UL35-containing nuclear bodies formation. Regulates viral
CC assembly by controlling the localization of the essential gB through
CC regulation of a retrograde transport pathway. This modulation occurs
CC via binding and inhibition of host sorting nexin 5/SNX5.
CC {ECO:0000250|UniProtKB:P16766}.
CC -!- SUBUNIT: [Isoform UL35]: Interacts with UL82. Interacts with isoform
CC UL35A. Interacts with host UBP7; this interaction significantly
CC inhibits the ability of USP7 to form nuclear bodies. Interacts with
CC host DCAF1 (via C-terminus). Interacts with host SNX5; this interaction
CC allows proper gB localization during viral assembly. Interacts wth host
CC TBK1; this interaction prevents type I interferon production.
CC {ECO:0000250|UniProtKB:P16766}.
CC -!- SUBUNIT: [Isoform UL35A]: Interacts with UL82. Interacts with isoform
CC UL35. Interacts with host UBP7; this interaction significantly inhibits
CC the ability of USP7 to form nuclear bodies. Interacts with host SNX5;
CC this interaction allows proper gB localization during viral assembly.
CC {ECO:0000250|UniProtKB:P16766}.
CC -!- SUBCELLULAR LOCATION: [Isoform UL35]: Virion tegument
CC {ECO:0000250|UniProtKB:P16766}. Host nucleus
CC {ECO:0000250|UniProtKB:P16766}. Host cytoplasm
CC {ECO:0000250|UniProtKB:P16766}. Note=Found in nuclear bodies.
CC {ECO:0000250|UniProtKB:P16766}.
CC -!- SUBCELLULAR LOCATION: [Isoform UL35A]: Host nucleus
CC {ECO:0000250|UniProtKB:P16766}. Host cytoplasm
CC {ECO:0000250|UniProtKB:P16766}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=UL35;
CC IsoId=F5HE12-1; Sequence=Displayed;
CC Name=UL35A;
CC IsoId=F5HE12-2; Sequence=VSP_044021;
CC -!- SIMILARITY: Belongs to the herpesviridae pp85 family. {ECO:0000305}.
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DR EMBL; AY446894; AAR31600.1; -; Genomic_DNA.
DR RefSeq; YP_081494.1; NC_006273.2.
DR SMR; F5HE12; -.
DR BioGRID; 1677983; 7.
DR PRIDE; F5HE12; -.
DR DNASU; 3077429; -.
DR GeneID; 3077429; -.
DR KEGG; vg:3077429; -.
DR Reactome; R-HSA-9609690; HCMV Early Events.
DR Reactome; R-HSA-9610379; HCMV Late Events.
DR Proteomes; UP000000938; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; ISS:UniProtKB.
DR GO; GO:0019033; C:viral tegument; TAS:Reactome.
DR GO; GO:0039695; P:DNA-templated viral transcription; ISS:UniProtKB.
DR GO; GO:0039722; P:suppression by virus of host toll-like receptor signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039723; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity; IEA:UniProtKB-KW.
DR InterPro; IPR006731; Herpes_pp85.
DR Pfam; PF04637; Herpes_pp85; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Host cytoplasm; Host nucleus;
KW Host-virus interaction; Inhibition of host innate immune response by virus;
KW Inhibition of host TBK1 by virus; Inhibition of host TLR pathway by virus;
KW Reference proteome; Viral immunoevasion; Virion; Virion tegument.
FT CHAIN 1..641
FT /note="Tegument protein UL35"
FT /id="PRO_0000418284"
FT REGION 353..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 500..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 587..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 500..564
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 588..602
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 626..641
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..447
FT /note="Missing (in isoform UL35A)"
FT /evidence="ECO:0000305"
FT /id="VSP_044021"
SQ SEQUENCE 641 AA; 72630 MW; 0347DFCF557C10F6 CRC64;
MAQGSRAPSG PPLPVLPVDD WLNFRVDLFG DEHRRLLLEM LTQGCSNFVG LLNFGVPSPV
YALEALVDFQ VRNAFMKVKP VAQEIIRICI LANHYRNSRD VLRDLRTQLD VLYSEPLKTR
LLRGLIRLCR AAQTGVKPED ISVHLGADDV TFGVLKRALV RLHRVRDALG LRASPEAEAR
YPRLTTYNLL FHPPPFTTVE AVDLCAENLS DVTQRRNRPL RCLTSIKRPG SRTLEDALND
MYLLLTLRHL QLRHALELQM MQDWVVERCN RLCDALYFCY TQAPETRQTF VTLVRGLELA
RQHSSPAFQP MLYNLLQLLT QLHEANVYLC PGYLHFSAYK LLKKIQSVSD ARERGEFGDE
DEEQENDGEP REAQLDLEAD PTAREGELFF FSKNLYGNGE VFRVPEQPSR YLRRRMFVER
PETLQIFYNF HEGKITTETY HLQRIYSMMI EGASRQTGLT PKRFMELLDR APLGQESEPE
ITEHRDLFAD VFRRPVTDAA SSSSASSSSS SASPNSVSLP SARSSSTRTT TPASTYTSAG
TSSTTGLLLS SSSLSGSHGI SSADLEQPPR QRRRMVSVTL FSPYSVAYSH HRRHRRRRSP
PPAPRGPAHT RFQGPDSMPS TSYGSDVEDP RDDLAENLRH L
