ID   UL49_HHV8P              Reviewed;         429 AA.
AC   F5HG20;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 21.
DE   RecName: Full=Protein ORF66;
GN   Name=ORF66;
OS   Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's
OS   sarcoma-associated herpesvirus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus.
OX   NCBI_TaxID=868565;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10400794; DOI=10.1128/jvi.73.8.6953-6963.1999;
RA   Glenn M., Rainbow L., Aurade F., Davison A., Schulz T.F.;
RT   "Identification of a spliced gene from Kaposi's sarcoma-associated
RT   herpesvirus encoding a protein with similarities to latent membrane
RT   proteins 1 and 2A of Epstein-Barr virus.";
RL   J. Virol. 73:6953-6963(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16760382; DOI=10.1099/vir.0.81919-0;
RA   Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.;
RT   "Kaposi's sarcoma-associated herpesvirus immune modulation: an overview.";
RL   J. Gen. Virol. 87:1781-1804(2006).
RN   [3]
RP   FUNCTION, INTERACTION WITH ORF34, AND SUBCELLULAR LOCATION.
RX   PubMed=28336944; DOI=10.1038/s41598-017-00401-7;
RA   Nishimura M., Watanabe T., Yagi S., Yamanaka T., Fujimuro M.;
RT   "Kaposi's sarcoma-associated herpesvirus ORF34 is essential for late gene
RT   expression and virus production.";
RL   Sci. Rep. 7:329-329(2017).
RN   [4]
RP   FUNCTION, INTERACTION WITH ORF34, DISRUPTION PHENOTYPE, AND INDUCTION.
RX   PubMed=31694948; DOI=10.1128/jvi.01300-19;
RA   Watanabe T., Nishimura M., Izumi T., Kuriyama K., Iwaisako Y., Hosokawa K.,
RA   Takaori-Kondo A., Fujimuro M.;
RT   "Kaposi's Sarcoma-Associated Herpesvirus ORF66 Is Essential for Late Gene
RT   Expression and Virus Production via Interaction with ORF34.";
RL   J. Virol. 94:0-0(2020).
CC   -!- FUNCTION: Participates in the expression of late viral mRNAs.
CC       {ECO:0000269|PubMed:28336944, ECO:0000269|PubMed:31694948}.
CC   -!- SUBUNIT: Interacts with ORF34. {ECO:0000269|PubMed:28336944,
CC       ECO:0000269|PubMed:31694948}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:28336944}. Host
CC       cytoplasm {ECO:0000269|PubMed:28336944}. Note=Mainly localizes in host
CC       nucleus and partially in host cytosol. {ECO:0000269|PubMed:28336944}.
CC   -!- INDUCTION: Expressed during the early stages of lytic infection.
CC       {ECO:0000269|PubMed:31694948}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant shows mainly attenuated late gene
CC       expression and decreased virus production while viral DNA replication
CC       remains unaffected. {ECO:0000269|PubMed:31694948}.
CC   -!- SIMILARITY: Belongs to the herpesviridae UL49 family. {ECO:0000305}.
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DR   EMBL; AF148805; ABD28921.1; -; Genomic_DNA.
DR   RefSeq; YP_001129423.1; NC_009333.1.
DR   PRIDE; F5HG20; -.
DR   DNASU; 4961523; -.
DR   GeneID; 4961523; -.
DR   KEGG; vg:4961523; -.
DR   Proteomes; UP000000942; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:InterPro.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR004339; UL49.
DR   Pfam; PF03117; Herpes_UL49_1; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Host nucleus; Reference proteome.
FT   CHAIN           1..429
FT                   /note="Protein ORF66"
FT                   /id="PRO_0000423767"
SQ   SEQUENCE   429 AA;  46983 MW;  4E56FD2AE070237E CRC64;
     MALDQRWDRF LVSWFGLDEA QLTAHRVFEG ENGVPVEEYV AFVIFGERGF QGNMPSWARH
     LLDRPSLAQA IAVLRAGSDT VAKQAQICAA QQLLGAHVWV VVTLSRAQAA DHARAIPRHV
     WAKYLSLPFS KACAQLCKLL ALCSRFPLVT CCSKPPPSLP WLRKKWHGPL PRRPLLEVPS
     PTRRGVAATE DGNGLGIGAA DTGLREALER VAPTVPCGNP FDAMLGSLCF LSLIKSRHVV
     LPACEQEGPG LVRNLGRRLL AYNVLSPCVS IPVICSRVAR AALAKRARCA RAVVCMECGH
     CLNFGRGKFH TVNFPPTNVF FSRDRKEKQF TICATTGRIY CSYCGSEHMR VYPLCDITGR
     GTLARVVIRA VLANNAALAI RDLDQTVSFV VPCLGTPDCE AALLKHRDVR GLLQLTSQLL
     EFCCGKCSS