UL56_HHV11
ID UL56_HHV11 Reviewed; 234 AA.
AC P10240; O09801;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2019, sequence version 2.
DT 02-JUN-2021, entry version 69.
DE RecName: Full=Protein UL56;
GN Name=UL56;
OS Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus.
OX NCBI_TaxID=10299;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2839594; DOI=10.1099/0022-1317-69-7-1531;
RA McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D.,
RA Perry L.J., Scott J.E., Taylor P.;
RT "The complete DNA sequence of the long unique region in the genome of
RT herpes simplex virus type 1.";
RL J. Gen. Virol. 69:1531-1574(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2846760; DOI=10.1099/0022-1317-69-11-2831;
RA Perry L.J., McGeoch D.J.;
RT "The DNA sequences of the long repeat region and adjoining parts of the
RT long unique region in the genome of herpes simplex virus type 1.";
RL J. Gen. Virol. 69:2831-2846(1988).
RN [3]
RP SEQUENCE REVISION.
RX PubMed=1662697; DOI=10.1099/0022-1317-72-12-3057;
RA McGeoch D.J., Cunningham C., McIntyre G., Dolan A.;
RT "Comparative sequence analysis of the long repeat regions and adjoining
RT parts of the long unique regions in the genomes of herpes simplex viruses
RT types 1 and 2.";
RL J. Gen. Virol. 72:3057-3075(1991).
RN [4]
RP INTERACTION WITH HOST ITCH, DOMAIN, AND MUTAGENESIS OF TYR-26; TYR-52 AND
RP TYR-147.
RX PubMed=29535361; DOI=10.1038/s41598-018-22682-2;
RA Koshizuka T., Kobayashi T., Ishioka K., Suzutani T.;
RT "Herpesviruses possess conserved proteins for interaction with Nedd4 family
RT ubiquitin E3 ligases.";
RL Sci. Rep. 8:4447-4447(2018).
CC -!- FUNCTION: Plays a role in the transport and release of virions form the
CC host cytoplasm to the extracellular space. Relocalizes host NEDD4, a
CC cytosolic E3 ligase, to cytoplasmic vesicles.
CC {ECO:0000250|UniProtKB:P28282}.
CC -!- SUBUNIT: Interacts with host ITCH; this interaction induces
CC ubiquitination and probably degradation of ITCH (PubMed:29535361).
CC Interacts with host NEDD4; this interaction increases NEDD4
CC ubiquitination (By similarity). Interacts with UL11 (By similarity).
CC {ECO:0000250|UniProtKB:P28282, ECO:0000269|PubMed:29535361}.
CC -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:P28282}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- DOMAIN: Late-budding domains (L domains) are short sequence motifs
CC essential for viral particle budding. They recruit proteins of the host
CC ESCRT machinery (Endosomal Sorting Complex Required for Transport) or
CC ESCRT-associated proteins. UL56 contains three L domains: the PPXY
CC motifs which are involved in the interaction with ITCH, a member of the
CC NEDD4 family. {ECO:0000269|PubMed:29535361}.
CC -!- SIMILARITY: Belongs to the herpesviridae UL56 family. {ECO:0000305}.
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DR EMBL; X14112; CAA32292.1; -; Genomic_DNA.
DR PIR; B30090; WMBEY6.
DR RefSeq; YP_009137132.1; NC_001806.2.
DR SMR; P10240; -.
DR BioGRID; 971453; 2.
DR PRIDE; P10240; -.
DR DNASU; 2703428; -.
DR GeneID; 2703428; -.
DR KEGG; vg:2703428; -.
DR Proteomes; UP000009294; Genome.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR007620; Herpes_UL56.
DR Pfam; PF04534; Herpes_UL56; 1.
PE 1: Evidence at protein level;
KW Host Golgi apparatus; Host membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..234
FT /note="Protein UL56"
FT /id="PRO_0000116123"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 158..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 23..26
FT /note="PPXY motif"
FT /evidence="ECO:0000269|PubMed:29535361"
FT MOTIF 49..52
FT /note="PPXY motif"
FT /evidence="ECO:0000269|PubMed:29535361"
FT MOTIF 144..147
FT /note="PPXY motif"
FT /evidence="ECO:0000269|PubMed:29535361"
FT COMPBIAS 43..58
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..97
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 158..179
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 26
FT /note="Y->A: Complete loss of interaction with host ITCH;
FT when associated with A-52 and A-147."
FT /evidence="ECO:0000269|PubMed:29535361"
FT MUTAGEN 52
FT /note="Y->A: Complete loss of interaction with host ITCH;
FT when associated with A-26 and A-147."
FT /evidence="ECO:0000269|PubMed:29535361"
FT MUTAGEN 147
FT /note="Y->A: Complete loss of interaction with host ITCH;
FT when associated with A-26 and A-52."
FT /evidence="ECO:0000269|PubMed:29535361"
SQ SEQUENCE 234 AA; 25321 MW; 9F58D7D18ABFA5A7 CRC64;
MASEAAQPDA GLWSAGNAFA DPPPPYDSLS GRNEGPFVVI DLDTPTDPPP PYSAGPLLSV
PIPPTSSGEG EASERGRSRQ AAQRAARRAR RRAERRAQRR SFGPGGLLAT PLFLPETRLV
APPDITRDLL SGLPTYAEAM SDHPPTYATV VAVRSTEQPS GALAPDDQRR TQNSGAWRPP
RVNSRELYRA QRAARGSSDH APYRRQGCCG VVWRHAVFGV VAIVVVIILV FLWR
