ID   UL84_HCMVA              Reviewed;         586 AA.
AC   P16727; Q7M6K7;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   02-JUN-2021, entry version 59.
DE   RecName: Full=Protein UL84;
GN   Name=UL84;
OS   Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX   NCBI_TaxID=10360;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2161319; DOI=10.1007/978-3-642-74980-3_6;
RA   Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R.,
RA   Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A.,
RA   Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.;
RT   "Analysis of the protein-coding content of the sequence of human
RT   cytomegalovirus strain AD169.";
RL   Curr. Top. Microbiol. Immunol. 154:125-169(1990).
RN   [2]
RP   GENOME REANNOTATION.
RX   PubMed=12533697; DOI=10.1099/vir.0.18606-0;
RA   Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA   McGeoch D.J., Hayward G.S.;
RT   "The human cytomegalovirus genome revisited: comparison with the chimpanzee
RT   cytomegalovirus genome.";
RL   J. Gen. Virol. 84:17-28(2003).
RN   [3]
RP   ERRATUM OF PUBMED:12533697.
RA   Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA   McGeoch D.J., Hayward G.S.;
RL   J. Gen. Virol. 84:1053-1053(2003).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=15452216; DOI=10.1128/jvi.78.20.10960-10966.2004;
RA   Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA   Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA   Shenk T., Smith R.D., Nelson J.A.;
RT   "Identification of proteins in human cytomegalovirus (HCMV) particles: the
RT   HCMV proteome.";
RL   J. Virol. 78:10960-10966(2004).
RN   [5]
RP   ERRATUM OF PUBMED:15452216.
RA   Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA   Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA   Shenk T., Smith R.D., Nelson J.A.;
RL   J. Virol. 78:13395-13395(2004).
RN   [6]
RP   INTERACTION WITH IE2.
RX   PubMed=7933141; DOI=10.1128/jvi.68.11.7549-7553.1994;
RA   Spector D.J., Tevethia M.J.;
RT   "Protein-protein interactions between human cytomegalovirus IE2-580aa and
RT   pUL84 in lytically infected cells.";
RL   J. Virol. 68:7549-7553(1994).
RN   [7]
RP   FUNCTION.
RX   PubMed=8892858; DOI=10.1128/jvi.70.11.7398-7413.1996;
RA   Sarisky R.T., Hayward G.S.;
RT   "Evidence that the UL84 gene product of human cytomegalovirus is essential
RT   for promoting oriLyt-dependent DNA replication and formation of replication
RT   compartments in cotransfection assays.";
RL   J. Virol. 70:7398-7413(1996).
RN   [8]
RP   SUBCELLULAR LOCATION, AND NUCLEAR LOCALIZATION SIGNAL.
RX   PubMed=12163612; DOI=10.1128/jvi.76.17.8931-8938.2002;
RA   Xu Y., Colletti K.S., Pari G.S.;
RT   "Human cytomegalovirus UL84 localizes to the cell nucleus via a nuclear
RT   localization signal and is a component of viral replication compartments.";
RL   J. Virol. 76:8931-8938(2002).
RN   [9]
RP   REGION.
RX   PubMed=17005707; DOI=10.1128/jvi.00995-06;
RA   Lischka P., Rauh C., Mueller R., Stamminger T.;
RT   "Human cytomegalovirus UL84 protein contains two nuclear export signals and
RT   shuttles between the nucleus and the cytoplasm.";
RL   J. Virol. 80:10274-10280(2006).
RN   [10]
RP   INTERACTION WITH UL44.
RX   PubMed=19457994; DOI=10.1128/jvi.00663-09;
RA   Strang B.L., Sinigalia E., Silva L.A., Coen D.M., Loregian A.;
RT   "Analysis of the association of the human cytomegalovirus DNA polymerase
RT   subunit UL44 with the viral DNA replication factor UL84.";
RL   J. Virol. 83:7581-7589(2009).
RN   [11]
RP   FUNCTION.
RX   PubMed=19631360; DOI=10.1016/j.virol.2009.06.035;
RA   Kagele D., Gao Y., Smallenburg K., Pari G.S.;
RT   "Interaction of HCMV UL84 with C/EBPalpha transcription factor binding
RT   sites within oriLyt is essential for lytic DNA replication.";
RL   Virology 392:16-23(2009).
RN   [12]
RP   INTERACTION WITH HOST HNRNPK.
RX   PubMed=22236369; DOI=10.1016/j.virol.2011.12.010;
RA   Kagele D., Rossetto C.C., Tarrant M.T., Pari G.S.;
RT   "Analysis of the interactions of viral and cellular factors with human
RT   cytomegalovirus lytic origin of replication, oriLyt.";
RL   Virology 424:106-114(2012).
CC   -!- FUNCTION: Plays an essential role in viral DNA replication. May
CC       participate in the DNA replication initiation by interacting with the
CC       origin of lytic replication, oriLyt and subsequently recruiting other
CC       viral/cellular factors. Additionally, interacts with and shuttles IRS1
CC       viral mRNA from the host nucleus to the cytoplasm.
CC       {ECO:0000269|PubMed:19631360, ECO:0000269|PubMed:8892858}.
CC   -!- SUBUNIT: Interacts with the DNA polymerase accessory subunit UL44.
CC       Interacts with HCMV major transcription-activating enzyme IE2.
CC       Interacts with host HNRNPK. {ECO:0000269|PubMed:19457994,
CC       ECO:0000269|PubMed:22236369, ECO:0000269|PubMed:7933141}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:12163612}. Host
CC       cytoplasm {ECO:0000269|PubMed:12163612}. Note=Shuttles between host
CC       nucleus and cytoplasm. In the nucleus, colocalizes with UL44 and IE2 in
CC       the viral DNA replication compartments.
CC   -!- SIMILARITY: Belongs to the HHV-5 UL84 protein family. {ECO:0000305}.
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DR   EMBL; X17403; CAA35358.1; -; Genomic_DNA.
DR   EMBL; BK000394; DAA00181.1; -; Genomic_DNA.
DR   PIR; S09848; WMBEDE.
DR   Proteomes; UP000008991; Genome.
DR   Proteomes; UP000008992; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR010436; Herpes_UL84.
DR   Pfam; PF06284; Cytomega_UL84; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Host nucleus; Reference proteome.
FT   CHAIN           1..586
FT                   /note="Protein UL84"
FT                   /id="PRO_0000116297"
FT   REGION          1..47
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          136..183
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           161..170
FT                   /note="Nuclear localization signal"
FT   MOTIF           228..237
FT                   /note="Nuclear export signal 1 (NES 1)"
FT   MOTIF           359..366
FT                   /note="Nuclear export signal 2 (NES 2)"
FT   COMPBIAS        136..151
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        154..183
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   586 AA;  65428 MW;  54AB912D6077223F CRC64;
     MPRVDPNLRN RARRPRARRG GGGGVGSNSS RHSGKCRRQR RALSAPPLTF LATTTTTTMM
     GVASTDDDSL LLKTPDELDK YSGSPQTILT LTDKHDIRQP RVHRGTYHLI QLHLDLRPEE
     LRDPFQILLS TPLQLGEAND ESQTAPATLQ EEETAASHEP EKKKEKQEKK EEDEDDRNDD
     RERGILCVVS NEDSDVRPAF SLFPARPGCH ILRSVIDQQL TRMAIVRLSL NLFALRIITP
     LLKRLPLRRK AAHHTALHDC LALHLPELTF EPTLDINNVT ENAASVADTA ESTDADLTPT
     LTVRVRHALC WHRVEGGISG PRGLTSRISA RLSETTAKTL GPSVFGRLEL DPNESPPDLT
     LSSLTLYQDG ILRFNVTCDR TEAPADPVAF RLRLRRETVR RPFFSDAPLP YFVPPRSGAA
     DEGLEVRVPY ELTLKNSHTL RIYRRFYGPY LGVFVPHNRQ GLKMPVTVWL PRSWLELTVL
     VSDENGATFP RDALLGRLYF ISSKHTLNRG CLSAMTHQVK STLHSRSTSH SPSQQQLSVL
     GASIALEDLL PMRLASPETE PQDCKLTENT TEKTSPVTLA MVCGDL