UL95_HHV8P
ID UL95_HHV8P Reviewed; 327 AA.
AC Q2HR98;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=Protein UL95 homolog;
GN Name=ORF34;
OS Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's
OS sarcoma-associated herpesvirus).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus.
OX NCBI_TaxID=868565;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10400794; DOI=10.1128/jvi.73.8.6953-6963.1999;
RA Glenn M., Rainbow L., Aurade F., Davison A., Schulz T.F.;
RT "Identification of a spliced gene from Kaposi's sarcoma-associated
RT herpesvirus encoding a protein with similarities to latent membrane
RT proteins 1 and 2A of Epstein-Barr virus.";
RL J. Virol. 73:6953-6963(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16760382; DOI=10.1099/vir.0.81919-0;
RA Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.;
RT "Kaposi's sarcoma-associated herpesvirus immune modulation: an overview.";
RL J. Gen. Virol. 87:1781-1804(2006).
RN [3]
RP FUNCTION, AND INTERACTION WITH ORF31.
RX PubMed=25810551; DOI=10.1128/jvi.00272-15;
RA Brulois K., Wong L.Y., Lee H.R., Sivadas P., Ensser A., Feng P., Gao S.J.,
RA Toth Z., Jung J.U.;
RT "Association of Kaposi's Sarcoma-Associated Herpesvirus ORF31 with ORF34
RT and ORF24 Is Critical for Late Gene Expression.";
RL J. Virol. 89:6148-6154(2015).
RN [4]
RP FUNCTION, AND INTERACTION WITH ORF24.
RX PubMed=26468530; DOI=10.1128/jvi.02157-15;
RA Davis Z.H., Hesser C.R., Park J., Glaunsinger B.A.;
RT "Interaction between ORF24 and ORF34 in the Kaposi's Sarcoma-Associated
RT Herpesvirus Late Gene Transcription Factor Complex Is Essential for Viral
RT Late Gene Expression.";
RL J. Virol. 90:599-604(2016).
RN [5]
RP FUNCTION, INTERACTION WITH ORF24; ORF31; ORF18; ORF23 AND ORF66,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=28336944; DOI=10.1038/s41598-017-00401-7;
RA Nishimura M., Watanabe T., Yagi S., Yamanaka T., Fujimuro M.;
RT "Kaposi's sarcoma-associated herpesvirus ORF34 is essential for late gene
RT expression and virus production.";
RL Sci. Rep. 7:329-329(2017).
RN [6]
RP FUNCTION, INTERACTION WITH HOST EPAS1, AND DISRUPTION PHENOTYPE.
RX PubMed=31189709; DOI=10.1128/jvi.00764-19;
RA Haque M., Kousoulas K.G.;
RT "The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Interacts and
RT Stabilizes HIF-2alpha via Binding to the HIF-2alpha bHLH and PAS Domains.";
RL J. Virol. 93:0-0(2019).
RN [7]
RP FUNCTION, AND INTERACTION WITH ORF66.
RX PubMed=31578296; DOI=10.1128/jvi.01299-19;
RA Didychuk A.L., Castaneda A.F., Kushnir L.O., Huang C.J., Glaunsinger B.A.;
RT "Conserved CxnC Motifs in Kaposi's Sarcoma-Associated Herpesvirus ORF66 Are
RT Required for Viral Late Gene Expression and Are Essential for Its
RT Interaction with ORF34.";
RL J. Virol. 94:0-0(2020).
CC -!- FUNCTION: Participates in the expression of late viral mRNAs in part by
CC interacting with ORF24 (PubMed:26468530, PubMed:25810551). Expressed
CC before viral DNA replication, assembles at the viral pre-replication
CC complexes (pre-RCs) and thus serves as a hub for recruiting a viral
CC transcription complex to ORF24 to promote late viral gene expression
CC (PubMed:28336944). Also plays a regulatory role in the viral life cycle
CC by regulating host transcriptional regulators HIF1A and EPAS1
CC (PubMed:31189709). {ECO:0000269|PubMed:26468530,
CC ECO:0000269|PubMed:28336944, ECO:0000269|PubMed:31189709,
CC ECO:0000269|PubMed:31578296}.
CC -!- SUBUNIT: Interacts with ORF24; this interaction may serve as a core
CC scaffold for the assembly of the viral transcription initiation complex
CC (PubMed:26468530, PubMed:28336944). Interacts with ORF66
CC (PubMed:28336944, PubMed:31578296). Interacts with ORF18
CC (PubMed:28336944). Interacts with ORF23 (PubMed:28336944). Interacts
CC with ORF31 (PubMed:28336944, PubMed:25810551). Interacts with host
CC EPAS1; this interaction stabilizes host EPAS1, ensuring its
CC transcriptional activity (PubMed:31189709).
CC {ECO:0000269|PubMed:25810551, ECO:0000269|PubMed:26468530,
CC ECO:0000269|PubMed:28336944, ECO:0000269|PubMed:31189709,
CC ECO:0000269|PubMed:31578296}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:28336944}.
CC Note=Recruited to nuclear replication compartments.
CC {ECO:0000269|PubMed:28336944}.
CC -!- DISRUPTION PHENOTYPE: Deletion results in reduced immediate early and
CC early lytic gene expression and blocked late gene expression, while
CC viral DNA replication seems not affected. {ECO:0000269|PubMed:28336944,
CC ECO:0000269|PubMed:31189709}.
CC -!- SIMILARITY: Belongs to the herpesviridae UL95 family. {ECO:0000305}.
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DR EMBL; AF148805; ABD28885.1; -; Genomic_DNA.
DR RefSeq; YP_001129387.1; NC_009333.1.
DR BioGRID; 1777007; 3.
DR PRIDE; Q2HR98; -.
DR DNASU; 4961504; -.
DR GeneID; 4961504; -.
DR KEGG; vg:4961504; -.
DR Proteomes; UP000000942; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR004280; Herpes_UL95.
DR Pfam; PF03038; Herpes_UL95; 1.
PE 1: Evidence at protein level;
KW Host nucleus; Reference proteome.
FT CHAIN 1..327
FT /note="Protein UL95 homolog"
FT /id="PRO_0000423808"
SQ SEQUENCE 327 AA; 36079 MW; 2BE6D7AE6D7BED5A CRC64;
MFALSSLVSE GDPEVTSRYV KGVQLALDLS ENTPGQFKLI ETPLNSFLLV SNVMPEVQPI
CSGLPALRPD FSNLHLPRLE KLQRVLGQGF GAAGEEIALD PSHVETHEKG QVFYNHYATE
EWTWALTLNK DALLREAVDG LCDPGTWKGL LPDDPLPLLW LLFNGPASFC RADCCLYKQH
CGYPGPVLLP GHMYAPKRDL LSFVNHALKY TKFLYGDFSG TWAAACRPPF ATSRIQRVVS
QMKIIDASDT YISHTCLLCH IYQQNSIIAG QGTHVGGILL LSGKGTQYIT GNVQTQRCPT
TGDYLIIPSY DIPAIITMIK ENGLNQL
