ID   ULA1_CAEEL              Reviewed;         541 AA.
AC   Q18217;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 2.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=NEDD8-activating enzyme E1 regulatory subunit;
DE   AltName: Full=Ubiquitin-like activation protein 1;
GN   Name=ula-1; ORFNames=C26E6.8;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   IDENTIFICATION, NOMENCLATURE, FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=10993680; DOI=10.1006/dbio.2000.9847;
RA   Jones D., Candido E.P.M.;
RT   "The NED-8 conjugating system in Caenorhabditis elegans is required for
RT   embryogenesis and terminal differentiation of the hypodermis.";
RL   Dev. Biol. 226:152-165(2000).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=19528325; DOI=10.1534/genetics.109.104885;
RA   Dorfman M., Gomes J.E., O'Rourke S., Bowerman B.;
RT   "Using RNA interference to identify specific modifiers of a temperature-
RT   sensitive, embryonic-lethal mutation in the Caenorhabditis elegans
RT   ubiquitin-like Nedd8 protein modification pathway E1-activating gene rfl-
RT   1.";
RL   Genetics 182:1035-1049(2009).
CC   -!- FUNCTION: Regulatory subunit of the dimeric uba-3-ula-1 E1 enzyme. E1
CC       activates NEDD8 by first adenylating its C-terminal glycine residue
CC       with ATP, thereafter linking this residue to the side chain of the
CC       catalytic cysteine, yielding a NEDD8-rfl-1 (uba-3) thioester and free
CC       AMP. E1 finally transfers NEDD8 to the catalytic cysteine of ubc-12
CC       (PubMed:10993680). Required for rfl-1 (uba-3) nuclear localization
CC       during early embryonic development (PubMed:19528325).
CC       {ECO:0000269|PubMed:10993680, ECO:0000269|PubMed:19528325}.
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SUBUNIT: Heterodimer of uba-3 and ula-1. The complex binds NEDD8 and
CC       ubc-12 (By similarity). {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes the formation of
CC       an everted vulva in the L4 stage, with the subsequent rupture of the
CC       animal during the L4-to-adult molt (PubMed:10993680). Loss of rfl-1
CC       nuclear localization in a 1- to 2-cell embryonic stages
CC       (PubMed:19528325). {ECO:0000269|PubMed:10993680,
CC       ECO:0000269|PubMed:19528325}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. ULA1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; FO080680; CCD65733.1; -; Genomic_DNA.
DR   PIR; F88444; F88444.
DR   RefSeq; NP_498037.2; NM_065636.4.
DR   AlphaFoldDB; Q18217; -.
DR   SMR; Q18217; -.
DR   BioGRID; 57300; 3.
DR   IntAct; Q18217; 1.
DR   STRING; 6239.C26E6.8; -.
DR   EPD; Q18217; -.
DR   PaxDb; Q18217; -.
DR   PeptideAtlas; Q18217; -.
DR   PRIDE; Q18217; -.
DR   EnsemblMetazoa; C26E6.8a.1; C26E6.8a.1; WBGene00006735.
DR   GeneID; 266650; -.
DR   KEGG; cel:CELE_C26E6.8; -.
DR   UCSC; C26E6.8.1; c. elegans.
DR   CTD; 266650; -.
DR   WormBase; C26E6.8a; CE29678; WBGene00006735; ula-1.
DR   eggNOG; KOG2016; Eukaryota.
DR   GeneTree; ENSGT00550000074901; -.
DR   HOGENOM; CLU_019618_2_1_1; -.
DR   InParanoid; Q18217; -.
DR   OMA; LHEICRY; -.
DR   PhylomeDB; Q18217; -.
DR   Reactome; R-CEL-8951664; Neddylation.
DR   UniPathway; UPA00885; -.
DR   PRO; PR:Q18217; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00006735; Expressed in germ line (C elegans) and 4 other tissues.
DR   ExpressionAtlas; Q18217; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0019781; F:NEDD8 activating enzyme activity; IBA:GO_Central.
DR   GO; GO:0043518; P:negative regulation of DNA damage response, signal transduction by p53 class mediator; IMP:UniProtKB.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR   GO; GO:0045116; P:protein neddylation; IBA:GO_Central.
DR   InterPro; IPR030667; APP-BP1.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR10953; PTHR10953; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   PIRSF; PIRSF039099; APP-BP1; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..541
FT                   /note="NEDD8-activating enzyme E1 regulatory subunit"
FT                   /id="PRO_0000194959"
SQ   SEQUENCE   541 AA;  60783 MW;  3A29704F87A0A623 CRC64;
     MLPFDPSTRY DRQVRLWGEE GQASIGSTSA CVLGSDSLAT EILKSLVLAG VQSFYVVDDA
     KVEQADIGQN FFLHADDIGR SRAEATLEKL TELNPSVSGS ASSQPPTALA MEDVEKLTTF
     SVVVAANQNE EIDTTFAKVL YNIRVPFICI KTFGLIGTIR ICIKEHTIAN SHEENPRPDL
     RLDAPFSKLI EMINETNLDE MTLEQLRHTP YILLHFKALE VFRKQRNDPE AFPSTTAERK
     ELQAILMSFR RSSEESGTKD SENFDEAKAA VIRAFQRTTI GSSVKSILSS PQCSTSTRPF
     WLICEALRRF VTENNNLLPL RGTLPDMTSD SSRYTRLATL FHEKALSDAQ EVLRLTREVE
     KERGVGDVIS DDVCYRFCKN ADRIRVQYGD VLDYNEETKA IVEKIRESNI DEETRNQKVD
     EATWMLLMRA VGRFQKEKGR YPGTNGVPVS IDAQDLKKRV EVLIREALKD EQDFTSISNK
     VTDTAIAEIC RFGAAELHVI SSYVGGIAAQ EIIKLATNQY VPIDNTFIFD GHTQESATFK
     F