ID   ULA1_CHICK              Reviewed;         535 AA.
AC   Q5ZIE6;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=NEDD8-activating enzyme E1 regulatory subunit;
DE   AltName: Full=APP-BP1;
DE   AltName: Full=Amyloid protein-binding protein 1;
GN   Name=NAE1; Synonyms=APPBP1; ORFNames=RCJMB04_27g6;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1
CC       activates NEDD8 by first adenylating its C-terminal glycine residue
CC       with ATP, thereafter linking this residue to the side chain of the
CC       catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1
CC       finally transfers NEDD8 to the catalytic cysteine of UBE2M (By
CC       similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SUBUNIT: Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and
CC       UBE2M (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. ULA1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AJ720838; CAG32497.1; -; mRNA.
DR   RefSeq; NP_001006129.1; NM_001006129.2.
DR   AlphaFoldDB; Q5ZIE6; -.
DR   SMR; Q5ZIE6; -.
DR   STRING; 9031.ENSGALP00000008356; -.
DR   PaxDb; Q5ZIE6; -.
DR   GeneID; 415792; -.
DR   KEGG; gga:415792; -.
DR   CTD; 8883; -.
DR   VEuPathDB; HostDB:geneid_415792; -.
DR   eggNOG; KOG2016; Eukaryota.
DR   InParanoid; Q5ZIE6; -.
DR   OrthoDB; 454386at2759; -.
DR   PhylomeDB; Q5ZIE6; -.
DR   UniPathway; UPA00885; -.
DR   PRO; PR:Q5ZIE6; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0019781; F:NEDD8 activating enzyme activity; IBA:GO_Central.
DR   GO; GO:0032446; P:protein modification by small protein conjugation; IBA:GO_Central.
DR   GO; GO:0045116; P:protein neddylation; IBA:GO_Central.
DR   InterPro; IPR030667; APP-BP1.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR10953; PTHR10953; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   PIRSF; PIRSF039099; APP-BP1; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..535
FT                   /note="NEDD8-activating enzyme E1 regulatory subunit"
FT                   /id="PRO_0000194956"
FT   REGION          332..345
FT                   /note="Interaction with UBA3"
FT                   /evidence="ECO:0000250"
FT   SITE            212
FT                   /note="Interaction with UBA3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   535 AA;  60484 MW;  643EFF4F3230A856 CRC64;
     MAQPGRASLK EQRYDRQLRL WGDHGQEALE SAHVCVINAT ATGTEILKNL VLPGIGSFTI
     VDGNRVSGED VGNNFFLQKS HIGQSRAQSA TELLQELNND VSGNFVEESP ETLLDNDPSF
     FNRFNLVVAT QLSESTVLRL AELLWNSNIP LLICRTYGLV GYMRIIIKEH PVVESHPDNA
     LEDLRLDKQF PELTEHIQSY DLDHMDKKDH SHTPWIVIVA KYLTKWFNEK SDQLPKSYKE
     KEAFRQLIRQ GILKNENGTP EDEENFEEAI KNVNTALNTT KIPRCIEEIF NDDCCVNLTE
     QSPSFWILVR AVKEFVANEG QGCLPVRGTI PDMIADSSKF IKLQNVYREK AKRDIAAVGN
     HAAKLLQSLG KAPESISERE LKLLCSNSAF LRVVRCRSLS EEYGLNTFNK DEIISNMDNP
     DSEVVLYLML RAVDRFYKQH GRYPGVYNYQ VEDDIGKLKS CLTSFLQEHG LSVLVKDDYV
     HEFCRYGAAE PHAIAAFMGG AAAQEIIKVI TGQFVIFNNT YIYSGMSQTS ATFQL