ID   ULA1_XENLA              Reviewed;         533 AA.
AC   Q6NTW6;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=NEDD8-activating enzyme E1 regulatory subunit;
DE   AltName: Full=APP-BP1;
DE   AltName: Full=Amyloid protein-binding protein 1;
GN   Name=nae1; Synonyms=appbp1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Regulatory subunit of the dimeric uba3-nae1 E1 enzyme. E1
CC       activates nedd8 by first adenylating its C-terminal glycine residue
CC       with ATP, thereafter linking this residue to the side chain of the
CC       catalytic cysteine, yielding a nedd8-uba3 thioester and free AMP. E1
CC       finally transfers nedd8 to the catalytic cysteine of ube2m (By
CC       similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SUBUNIT: Heterodimer of uba3 and nae1. The complex binds nedd8 and
CC       ube2m (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. ULA1
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC068838; AAH68838.1; -; mRNA.
DR   RefSeq; NP_001084652.1; NM_001091183.1.
DR   AlphaFoldDB; Q6NTW6; -.
DR   SMR; Q6NTW6; -.
DR   DNASU; 414612; -.
DR   GeneID; 414612; -.
DR   KEGG; xla:414612; -.
DR   CTD; 414612; -.
DR   Xenbase; XB-GENE-971671; nae1.S.
DR   OrthoDB; 454386at2759; -.
DR   UniPathway; UPA00885; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 414612; Expressed in muscle tissue and 19 other tissues.
DR   GO; GO:0019781; F:NEDD8 activating enzyme activity; IEA:InterPro.
DR   GO; GO:0045116; P:protein neddylation; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR030667; APP-BP1.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR10953; PTHR10953; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   PIRSF; PIRSF039099; APP-BP1; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..533
FT                   /note="NEDD8-activating enzyme E1 regulatory subunit"
FT                   /id="PRO_0000194957"
FT   REGION          330..343
FT                   /note="Interaction with uba3"
FT                   /evidence="ECO:0000250"
FT   SITE            210
FT                   /note="Interaction with uba3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   533 AA;  60625 MW;  815C6FB09F217272 CRC64;
     MAQVVHIKEQ KYDRQLRLWG DHGQEALESA HVCLINATAT GTEILKNLVL PGIGLFTIVD
     GNVVAGEDVG NNFFLQKESI GKNRAQTSME LLQELNDDVT GNFVPESPEQ LLDKDPSFFC
     KFTMVISTQL PESTLLRLAE TLWDSNIPLL VCRAYGFVGY MRIIVKEHTV IESHPDNALE
     DLRLDQPFPE LREHLQSYDL DHMERKDHSH TPWIIVVAKY LDKWRSENGG QMPKSYKEKE
     SFRDLIRQGI LKNENGVPED EENFEEAIKN VNTALNITKV SSSVEEILND DRCTNLTHQS
     TSFWILARAV KEFMAMEGKG NLPLRGTIPD MIADSDKFIK LQNIYREKAK KDASAVESCV
     SKLLQSVGRP PESISERDIR LFCRNCAFLR VVRCRSLEEE YGLDTAKKDD IVSLMENPDN
     EIVLYLMLRA VDRFQKQLGR YPGIYNYQIE SDIGKLKSCL NGLLQEYGLS LTVKDEYIQE
     FCRYGAAEPH TIASFLGGAA AQEAIKIITK QFVIFNNTFI YNAMLQTSAT FQL