ID   CA189_CONAJ             Reviewed;          60 AA.
AC   P0CB12;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Alpha-conotoxin-like 289;
DE   Flags: Precursor;
OS   Conus ammiralis (Admiral cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX   NCBI_TaxID=97188;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RA   Watkins M., Hillyard D.R., McIntosh M.J., Jones R.M., Olivera B.M.;
RT   "Alpha-conotoxin peptides.";
RL   Patent number EP1852440, 07-NOV-2007.
CC   -!- FUNCTION: Alpha-conotoxins act on postsynaptic membranes, they bind to
CC       the nicotinic acetylcholine receptors (nAChR) and thus inhibit them.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct. {ECO:0000305}.
CC   -!- DOMAIN: The cysteine framework is I (CC-C-C). Alpha4/7 pattern.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the conotoxin A superfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FB300119; CAR81591.1; -; Unassigned_DNA.
DR   AlphaFoldDB; P0CB12; -.
DR   ConoServer; 2943; Ai1.1 precursor.
DR   ConoServer; 3454; Sequence 289 from Patent EP1852440.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR009958; Conotoxin_a-typ.
DR   InterPro; IPR018072; Conotoxin_a-typ_CS.
DR   Pfam; PF07365; Toxin_8; 1.
DR   PROSITE; PS60014; ALPHA_CONOTOXIN; 1.
PE   3: Inferred from homology;
KW   Acetylcholine receptor inhibiting toxin; Amidation;
KW   Cleavage on pair of basic residues; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Postsynaptic neurotoxin;
KW   Pyrrolidone carboxylic acid; Secreted; Signal; Toxin.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   PROPEP          17..42
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000380626"
FT   PEPTIDE         43..59
FT                   /note="Alpha-conotoxin-like 289"
FT                   /id="PRO_0000380627"
FT   REGION          47..49
FT                   /note="Ser-Xaa-Pro motif, crucial for potent interaction
FT                   with nAChR"
FT                   /evidence="ECO:0000250|UniProtKB:P56636"
FT   MOD_RES         43
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         59
FT                   /note="Cysteine amide"
FT                   /evidence="ECO:0000250"
FT   DISULFID        45..51
FT                   /evidence="ECO:0000250|UniProtKB:P56636"
FT   DISULFID        46..59
FT                   /evidence="ECO:0000250|UniProtKB:P56636"
SQ   SEQUENCE   60 AA;  6452 MW;  4EE463147E28A69B CRC64;
     MFTVFLLVVL ATTVVSFTSD RAFRGRNAAA KASGLVGLTD KRQECCSYPA CNLDHPELCG