ID   ULAR_ECOLI              Reviewed;         251 AA.
AC   P0A9W0; P39299; Q2M6B4; Q6IU17;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=HTH-type transcriptional regulator UlaR;
GN   Name=ulaR; Synonyms=yjfQ; OrderedLocusNames=b4191, JW4149;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-202.
RC   STRAIN=B / Bc251;
RX   PubMed=12664169; DOI=10.1007/s00239-002-2423-0;
RA   Lenski R.E., Winkworth C.L., Riley M.A.;
RT   "Rates of DNA sequence evolution in experimental populations of Escherichia
RT   coli during 20,000 generations.";
RL   J. Mol. Evol. 56:498-508(2003).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=12374842; DOI=10.1128/jb.184.21.6065-6068.2002;
RA   Campos E., Aguilar J., Baldoma L., Badia J.;
RT   "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is
RT   involved in L-ascorbate metabolism in Escherichia coli.";
RL   J. Bacteriol. 184:6065-6068(2002).
RN   [6]
RP   FUNCTION.
RX   PubMed=12644495; DOI=10.1128/jb.185.7.2243-2250.2003;
RA   Zhang Z., Aboulwafa M., Smith M.H., Saier M.H. Jr.;
RT   "The ascorbate transporter of Escherichia coli.";
RL   J. Bacteriol. 185:2243-2250(2003).
RN   [7]
RP   FUNCTION.
RX   PubMed=14996803; DOI=10.1128/jb.186.6.1720-1728.2004;
RA   Campos E., Baldoma L., Aguilar J., Badia J.;
RT   "Regulation of expression of the divergent ulaG and ulaABCDEF operons
RT   involved in L-ascorbate dissimilation in Escherichia coli.";
RL   J. Bacteriol. 186:1720-1728(2004).
CC   -!- FUNCTION: Represses ulaG and the ulaABCDEF operon. Two ulaR binding
CC       sites have been identified in each promoter. Full activity requires
CC       simultaneous interaction of UlaR with both divergent promoters and
CC       seems to be dependent on repressor-mediated DNA loop formation, which
CC       is helped by the action of integration host factor.
CC       {ECO:0000269|PubMed:12374842, ECO:0000269|PubMed:12644495,
CC       ECO:0000269|PubMed:14996803}.
CC   -!- INTERACTION:
CC       P0A9W0; P0A6F5: groEL; NbExp=4; IntAct=EBI-560926, EBI-543750;
CC       P0A9W0; P76594: patZ; NbExp=4; IntAct=EBI-560926, EBI-557388;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene show a more rapid
CC       utilization of L-ascorbate and are able to grow on L-ascorbate under
CC       microaerophilic conditions. {ECO:0000269|PubMed:12644495}.
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DR   EMBL; U14003; AAA97087.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC77148.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78192.1; -; Genomic_DNA.
DR   EMBL; AY625132; AAT42486.1; -; Genomic_DNA.
DR   PIR; S56416; S56416.
DR   RefSeq; NP_418612.1; NC_000913.3.
DR   RefSeq; WP_000133631.1; NZ_STEB01000013.1.
DR   AlphaFoldDB; P0A9W0; -.
DR   SMR; P0A9W0; -.
DR   BioGRID; 4262711; 9.
DR   BioGRID; 852998; 1.
DR   DIP; DIP-47874N; -.
DR   IntAct; P0A9W0; 41.
DR   STRING; 511145.b4191; -.
DR   jPOST; P0A9W0; -.
DR   PaxDb; P0A9W0; -.
DR   PRIDE; P0A9W0; -.
DR   EnsemblBacteria; AAC77148; AAC77148; b4191.
DR   EnsemblBacteria; BAE78192; BAE78192; BAE78192.
DR   GeneID; 67414809; -.
DR   GeneID; 948706; -.
DR   KEGG; ecj:JW4149; -.
DR   KEGG; eco:b4191; -.
DR   PATRIC; fig|1411691.4.peg.2510; -.
DR   EchoBASE; EB2384; -.
DR   eggNOG; COG1349; Bacteria.
DR   HOGENOM; CLU_060699_3_2_6; -.
DR   OMA; RYDFFNF; -.
DR   PhylomeDB; P0A9W0; -.
DR   BioCyc; EcoCyc:G7854-MON; -.
DR   PRO; PR:P0A9W0; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IMP:EcoCyc.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEP:EcoCyc.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01563; HTH_type_UlaR; 1.
DR   InterPro; IPR014036; DeoR_C.
DR   InterPro; IPR001034; DeoR_HTH.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   InterPro; IPR018356; Tscrpt_reg_HTH_DeoR_CS.
DR   InterPro; IPR023711; Tscrpt_reg_HTH_UlaR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00455; DeoRC; 1.
DR   Pfam; PF08220; HTH_DeoR; 1.
DR   PRINTS; PR00037; HTHLACR.
DR   SMART; SM00420; HTH_DEOR; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00894; HTH_DEOR_1; 1.
DR   PROSITE; PS51000; HTH_DEOR_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..251
FT                   /note="HTH-type transcriptional regulator UlaR"
FT                   /id="PRO_0000050277"
FT   DOMAIN          3..58
FT                   /note="HTH deoR-type"
FT   DNA_BIND        20..39
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   251 AA;  27602 MW;  797396226CFE01D6 CRC64;
     MTEAQRHQIL LEMLAQLGFV TVEKVVERLG ISPATARRDI NKLDESGKLK KVRNGAEAIT
     QQRPRWTPMN LHQAQNHDEK VRIAKAASQL VNPGESVVIN CGSTAFLLGR EMCGKPVQII
     TNYLPLANYL IDQEHDSVII MGGQYNKSQS ITLSPQGSEN SLYAGHWMFT SGKGLTAEGL
     YKTDMLTAMA EQKMLSVVGK LVVLVDSSKI GERAGMLFSR ADQIDMLITG KNANPEILQQ
     LEAQGVSILR V