UME6_CANAL
ID UME6_CANAL Reviewed; 843 AA.
AC Q59MD2; A0A1D8PDR7; Q59ME1;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Transcriptional regulatory protein UME6;
GN Name=UME6; OrderedLocusNames=CAALFM_C106280CA;
GN ORFNames=CaO19.1822, CaO19.9381;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP INDUCTION.
RX PubMed=15554973; DOI=10.1111/j.1365-2958.2004.04350.x;
RA Bensen E.S., Martin S.J., Li M., Berman J., Davis D.A.;
RT "Transcriptional profiling in Candida albicans reveals new adaptive
RT responses to extracellular pH and functions for Rim101p.";
RL Mol. Microbiol. 54:1335-1351(2004).
RN [5]
RP IDENTIFICATION.
RX PubMed=18629206; DOI=10.1002/cfg.492;
RA Maicas S., Moreno I., Nieto A., Gomez M., Sentandreu R., Valentin E.;
RT "In silico analysis for transcription factors with Zn(II)(2)C(6) binuclear
RT cluster DNA-binding domains in Candida albicans.";
RL Comp. Funct. Genomics 6:345-356(2005).
RN [6]
RP INDUCTION.
RX PubMed=15814840; DOI=10.1091/mbc.e05-01-0073;
RA Kadosh D., Johnson A.D.;
RT "Induction of the Candida albicans filamentous growth program by relief of
RT transcriptional repression: a genome-wide analysis.";
RL Mol. Biol. Cell 16:2903-2912(2005).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=18216277; DOI=10.1091/mbc.e07-11-1110;
RA Banerjee M., Thompson D.S., Lazzell A., Carlisle P.L., Pierce C.,
RA Monteagudo C., Lopez-Ribot J.L., Kadosh D.;
RT "UME6, a novel filament-specific regulator of Candida albicans hyphal
RT extension and virulence.";
RL Mol. Biol. Cell 19:1354-1365(2008).
RN [8]
RP INDUCTION.
RX PubMed=18321992; DOI=10.1091/mbc.e07-09-0960;
RA Goyard S., Knechtle P., Chauvel M., Mallet A., Prevost M.C., Proux C.,
RA Coppee J.Y., Schwartz P., Dromer F., Park H., Filler S.G., Janbon G.,
RA d'Enfert C.;
RT "The Yak1 kinase is involved in the initiation and maintenance of hyphal
RT growth in Candida albicans.";
RL Mol. Biol. Cell 19:2251-2266(2008).
RN [9]
RP FUNCTION, AND INDUCTION.
RX PubMed=19054126; DOI=10.1111/j.1567-1364.2008.00459.x;
RA Zeidler U., Lettner T., Lassnig C., Muller M., Lajko R., Hintner H.,
RA Breitenbach M., Bito A.;
RT "UME6 is a crucial downstream target of other transcriptional regulators of
RT true hyphal development in Candida albicans.";
RL FEMS Yeast Res. 9:126-142(2009).
RN [10]
RP FUNCTION.
RX PubMed=19116272; DOI=10.1073/pnas.0804061106;
RA Carlisle P.L., Banerjee M., Lazzell A., Monteagudo C., Lopez-Ribot J.L.,
RA Kadosh D.;
RT "Expression levels of a filament-specific transcriptional regulator are
RT sufficient to determine Candida albicans morphology and virulence.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:599-604(2009).
RN [11]
RP FUNCTION.
RX PubMed=20656912; DOI=10.1128/ec.00046-10;
RA Carlisle P.L., Kadosh D.;
RT "Candida albicans Ume6, a filament-specific transcriptional regulator,
RT directs hyphal growth via a pathway involving Hgc1 cyclin-related
RT protein.";
RL Eukaryot. Cell 9:1320-1328(2010).
RN [12]
RP FUNCTION.
RX PubMed=20870878; DOI=10.1128/ec.00029-10;
RA Palmer G.E.;
RT "Endosomal and AP-3-dependent vacuolar trafficking routes make additive
RT contributions to Candida albicans hyphal growth and pathogenesis.";
RL Eukaryot. Cell 9:1755-1765(2010).
RN [13]
RP FUNCTION.
RX PubMed=20360962; DOI=10.1371/journal.ppat.1000828;
RA Uppuluri P., Chaturvedi A.K., Srinivasan A., Banerjee M.,
RA Ramasubramaniam A.K., Kohler J.R., Kadosh D., Lopez-Ribot J.L.;
RT "Dispersion as an important step in the Candida albicans biofilm
RT developmental cycle.";
RL PLoS Pathog. 6:E1000828-E1000828(2010).
RN [14]
RP INDUCTION.
RX PubMed=22544909; DOI=10.1128/ec.00103-12;
RA Fanning S., Xu W., Solis N., Woolford C.A., Filler S.G., Mitchell A.P.;
RT "Divergent targets of Candida albicans biofilm regulator Bcr1 in vitro and
RT in vivo.";
RL Eukaryot. Cell 11:896-904(2012).
RN [15]
RP FUNCTION.
RX PubMed=22276126; DOI=10.1371/journal.pone.0029707;
RA Du H., Guan G., Xie J., Sun Y., Tong Y., Zhang L., Huang G.;
RT "Roles of Candida albicans Gat2, a GATA-type zinc finger transcription
RT factor, in biofilm formation, filamentous growth and virulence.";
RL PLoS ONE 7:E29707-E29707(2012).
RN [16]
RP FUNCTION, AND INDUCTION.
RX PubMed=22536157; DOI=10.1371/journal.ppat.1002663;
RA Lu Y., Su C., Liu H.;
RT "A GATA transcription factor recruits Hda1 in response to reduced Tor1
RT signaling to establish a hyphal chromatin state in Candida albicans.";
RL PLoS Pathog. 8:E1002663-E1002663(2012).
RN [17]
RP INDUCTION.
RX PubMed=23125349; DOI=10.1128/ec.00236-12;
RA Pierce J.V., Dignard D., Whiteway M., Kumamoto C.A.;
RT "Normal adaptation of Candida albicans to the murine gastrointestinal tract
RT requires Efg1p-dependent regulation of metabolic and host defense genes.";
RL Eukaryot. Cell 12:37-49(2013).
RN [18]
RP FUNCTION.
RX PubMed=23223035; DOI=10.1128/ec.00163-12;
RA Banerjee M., Uppuluri P., Zhao X.R., Carlisle P.L., Vipulanandan G.,
RA Villar C.C., Lopez-Ribot J.L., Kadosh D.;
RT "Expression of UME6, a key regulator of Candida albicans hyphal
RT development, enhances biofilm formation via Hgc1- and Sun41-dependent
RT mechanisms.";
RL Eukaryot. Cell 12:224-232(2013).
RN [19]
RP FUNCTION.
RX PubMed=23913543; DOI=10.1128/ec.00118-13;
RA Rane H.S., Bernardo S.M., Raines S.M., Binder J.L., Parra K.J., Lee S.A.;
RT "Candida albicans VMA3 is necessary for V-ATPase assembly and function and
RT contributes to secretion and filamentation.";
RL Eukaryot. Cell 12:1369-1382(2013).
RN [20]
RP INDUCTION.
RX PubMed=23210679; DOI=10.3109/13693786.2012.743051;
RA Hsu C.C., Lai W.L., Chuang K.C., Lee M.H., Tsai Y.C.;
RT "The inhibitory activity of linalool against the filamentous growth and
RT biofilm formation in Candida albicans.";
RL Med. Mycol. 51:473-482(2013).
RN [21]
RP FUNCTION.
RX PubMed=23242994; DOI=10.1091/mbc.e12-01-0065;
RA Carlisle P.L., Kadosh D.;
RT "A genome-wide transcriptional analysis of morphology determination in
RT Candida albicans.";
RL Mol. Biol. Cell 24:246-260(2013).
RN [22]
RP FUNCTION.
RX PubMed=23844834; DOI=10.1111/mmi.12327;
RA Schillig R., Morschhauser J.;
RT "Analysis of a fungus-specific transcription factor family, the Candida
RT albicans zinc cluster proteins, by artificial activation.";
RL Mol. Microbiol. 89:1003-1017(2013).
RN [23]
RP INDUCTION.
RX PubMed=23505370; DOI=10.1371/journal.ppat.1003210;
RA Si H., Hernday A.D., Hirakawa M.P., Johnson A.D., Bennett R.J.;
RT "Candida albicans white and opaque cells undergo distinct programs of
RT filamentous growth.";
RL PLoS Pathog. 9:E1003210-E1003210(2013).
RN [24]
RP INDUCTION.
RX PubMed=23966855; DOI=10.1371/journal.ppat.1003519;
RA Znaidi S., Nesseir A., Chauvel M., Rossignol T., d'Enfert C.;
RT "A comprehensive functional portrait of two heat shock factor-type
RT transcriptional regulators involved in Candida albicans morphogenesis and
RT virulence.";
RL PLoS Pathog. 9:E1003519-E1003519(2013).
CC -!- FUNCTION: Component of the RPD3C(L) histone deacetylase complex (HDAC)
CC responsible for the deacetylation of lysine residues on the N-terminal
CC part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation
CC gives a tag for epigenetic repression and plays an important role in
CC transcriptional regulation, cell cycle progression and developmental
CC events (By similarity). UME6 controls the level and duration of gene
CC expression in the filamentous growth program such as HGC1; and is
CC specifically important for hyphal elongation and germ tube formation.
CC Promotes filamentous biofilms and is required for adherence to surfaces
CC such as plastic. Plays an important role in virulence. {ECO:0000250,
CC ECO:0000269|PubMed:18216277, ECO:0000269|PubMed:19054126,
CC ECO:0000269|PubMed:19116272, ECO:0000269|PubMed:20360962,
CC ECO:0000269|PubMed:20656912, ECO:0000269|PubMed:20870878,
CC ECO:0000269|PubMed:22276126, ECO:0000269|PubMed:22536157,
CC ECO:0000269|PubMed:23223035, ECO:0000269|PubMed:23242994,
CC ECO:0000269|PubMed:23844834, ECO:0000269|PubMed:23913543}.
CC -!- SUBUNIT: Component of the RPD3C(L) complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- INDUCTION: Up-regulated during growth in alkaline conditions, in
CC response to various filament-inducing conditions, and during
CC oropharyngeal candidiasis. Induced in both white and opaque cells.
CC Induced by SFL2 and repressed by RFG1, SFL1, and by NRG1 under non-
CC filament-inducing conditions. Also repressed by linalool. Expression is
CC also regulated by BRG1 and EFG1. {ECO:0000269|PubMed:15554973,
CC ECO:0000269|PubMed:15814840, ECO:0000269|PubMed:18216277,
CC ECO:0000269|PubMed:18321992, ECO:0000269|PubMed:19054126,
CC ECO:0000269|PubMed:22536157, ECO:0000269|PubMed:22544909,
CC ECO:0000269|PubMed:23125349, ECO:0000269|PubMed:23210679,
CC ECO:0000269|PubMed:23505370, ECO:0000269|PubMed:23966855}.
CC -!- DISRUPTION PHENOTYPE: Leads to attenuated virulence and defective
CC hyphal extension in a mouse model of systemic candidiasis.
CC {ECO:0000269|PubMed:18216277}.
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DR EMBL; CP017623; AOW26289.1; -; Genomic_DNA.
DR RefSeq; XP_710873.2; XM_705781.2.
DR AlphaFoldDB; Q59MD2; -.
DR SMR; Q59MD2; -.
DR STRING; 237561.Q59MD2; -.
DR PRIDE; Q59MD2; -.
DR GeneID; 3647522; -.
DR KEGG; cal:CAALFM_C106280CA; -.
DR CGD; CAL0000177314; UME6.
DR VEuPathDB; FungiDB:C1_06280C_A; -.
DR eggNOG; ENOG502RX7Y; Eukaryota.
DR HOGENOM; CLU_017130_0_0_1; -.
DR InParanoid; Q59MD2; -.
DR OrthoDB; 1481639at2759; -.
DR PHI-base; PHI:3502; -.
DR PRO; PR:Q59MD2; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 2: Evidence at transcript level;
KW Cell adhesion; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Virulence; Zinc.
FT CHAIN 1..843
FT /note="Transcriptional regulatory protein UME6"
FT /id="PRO_0000424863"
FT DNA_BIND 768..795
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 102..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 164..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 401..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 580..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..342
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 401..443
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 452..470
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 604..630
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 659..686
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 688..750
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 843 AA; 91749 MW; D57AADB7C3B137D8 CRC64;
MITHMVTPDS TSSAPNSPYG EDTIKLNSSV ISSSSPVRST SYLQNLQLQQ QFSQLQFLQL
QQQQQQQDQQ LALQQQQQQQ QSQSQQAQPY ANPYFQTPID QLFQFDNNPN SSFNPTRPPN
IHTNQPYVSP YLHSAPLTNH SDLQPQPPPA SLSDSELGLG LVKQQQDSLP PQQQAQSQPT
PLLNQQHPFY NEYINDMPST QHPYLIFNNT NAAHSTTSLP NLDAANPSSI LPQSALNQSE
YYKVTNGSMS NDPQPSTFLY NLHNHSADLV MNNDLEFSNA SFDSIGGVSV SAAATGAGAG
AGDYSHTNIT SNFAQTNLHS SSQQSGVLPP PPSLPPPPTS LPSQQSQNQY LDVVPPQQLT
NNALKTEANS PLITDSANFT TSQPQFENTI LEQSPIFSNN TILSGIDEPS KEKSPNMLNS
TLPLSKSTPH HIYQPSSSSS SHVRHNKITK KSSLSRLNTS SKKNLRANLV HSLSTPTELD
DANNNNNDIV TPPKTLSPLL RKKTSFINVK KENDIDDSHD INCKNFSIDI DDMDDEDVLS
DFSQPPPPIG GVSNDTMRSL APVGGVGLGS SGNITGYRSL SNASSTSSHG SHHGLPVMNI
FRHNPDSTKP STPSPILSTA SSSSTLSNKK VVKKLRGRKS KTKMNKSSIK TEQVVNEEPQ
VKNYKDDNDN DNDNGHGHDH DDENGGVSSD VGTGSNSTPS TTTKGKKMKL NVDLNNGGGS
GTGSGSCSDE NTSGNGTNTN TDSTSTSNPM VKKKHTRRRL LPRSKKGCWI CRIKHLKCDE
VTPICGGCAK FGLQCDYSSE KPAYVTDKIL RQKKLTEVSL IRKRNQAKTK ISRKKSSNDI
TND
