UMP1_YEAST
ID UMP1_YEAST Reviewed; 148 AA.
AC P38293; D6VQG9;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Proteasome maturation factor UMP1;
GN Name=UMP1; OrderedLocusNames=YBR173C; ORFNames=YBR1234;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YHP500;
RX PubMed=9491890; DOI=10.1016/s0092-8674(00)80942-3;
RA Ramos P.C., Hoeckendorff J., Johnson E.S., Varshavsky A., Dohmen R.J.;
RT "Ump1p is required for proper maturation of the 20S proteasome and becomes
RT its substrate upon completion of the assembly.";
RL Cell 92:489-499(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Short-lived chaperone present in the precursor form of the
CC 20S proteasome and absent in the mature complex. Required for the
CC correct assembly and enzymatic activation of the proteasome. Also
CC prevents premature processing of the PRE2 propeptide.
CC -!- PTM: Seems to be degraded by the proteasome upon its formation.
CC -!- MISCELLANEOUS: Present with 2740 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the POMP/UMP1 family. {ECO:0000305}.
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DR EMBL; AJ002557; CAA05556.1; -; Genomic_DNA.
DR EMBL; Z36042; CAA85134.1; -; Genomic_DNA.
DR EMBL; AY558126; AAS56452.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07289.1; -; Genomic_DNA.
DR PIR; S46044; S46044.
DR RefSeq; NP_009732.1; NM_001178521.1.
DR PDB; 7LS6; EM; 3.17 A; H=1-148.
DR PDB; 7LSX; EM; 3.61 A; H=1-148.
DR PDBsum; 7LS6; -.
DR PDBsum; 7LSX; -.
DR AlphaFoldDB; P38293; -.
DR BMRB; P38293; -.
DR SMR; P38293; -.
DR BioGRID; 32873; 305.
DR DIP; DIP-2815N; -.
DR IntAct; P38293; 5.
DR MINT; P38293; -.
DR STRING; 4932.YBR173C; -.
DR iPTMnet; P38293; -.
DR PaxDb; P38293; -.
DR PRIDE; P38293; -.
DR EnsemblFungi; YBR173C_mRNA; YBR173C; YBR173C.
DR GeneID; 852471; -.
DR KEGG; sce:YBR173C; -.
DR SGD; S000000377; UMP1.
DR VEuPathDB; FungiDB:YBR173C; -.
DR eggNOG; KOG3061; Eukaryota.
DR GeneTree; ENSGT00390000010734; -.
DR HOGENOM; CLU_100687_0_1_1; -.
DR InParanoid; P38293; -.
DR OMA; RETEVTW; -.
DR BioCyc; YEAST:G3O-29121-MON; -.
DR PRO; PR:P38293; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38293; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IDA:SGD.
DR GO; GO:0043248; P:proteasome assembly; IMP:SGD.
DR GO; GO:0090363; P:regulation of proteasome core complex assembly; IMP:CACAO.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:SGD.
DR DisProt; DP01140; -.
DR InterPro; IPR008012; Ump1.
DR PANTHER; PTHR12828; PTHR12828; 1.
DR Pfam; PF05348; UMP1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Reference proteome.
FT CHAIN 1..148
FT /note="Proteasome maturation factor UMP1"
FT /id="PRO_0000065720"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT TURN 32..35
FT /evidence="ECO:0007829|PDB:7LS6"
FT TURN 37..40
FT /evidence="ECO:0007829|PDB:7LS6"
FT HELIX 44..47
FT /evidence="ECO:0007829|PDB:7LS6"
FT HELIX 54..57
FT /evidence="ECO:0007829|PDB:7LS6"
FT HELIX 60..76
FT /evidence="ECO:0007829|PDB:7LS6"
FT HELIX 79..94
FT /evidence="ECO:0007829|PDB:7LS6"
FT HELIX 105..110
FT /evidence="ECO:0007829|PDB:7LS6"
FT HELIX 119..122
FT /evidence="ECO:0007829|PDB:7LS6"
FT STRAND 132..135
FT /evidence="ECO:0007829|PDB:7LS6"
FT HELIX 138..146
FT /evidence="ECO:0007829|PDB:7LS6"
SQ SEQUENCE 148 AA; 16760 MW; 7C774DD40F3FCD7C CRC64;
MNIVPQDTFK SQVSTDQDKS VLSSAVPSLP DTLRQQEGGA VPLSTQLNDR HPLESTLKNW
ETTQRQRQME QYRQIFGIAE PMKRTMEMEI VNRTDFNPLS TNGSIHRDIL LNKECSIDWE
DVYPGTGLQA STMVGDDVHS KIEKQLGI
