ID   UMPS2_ORYSJ             Reviewed;         475 AA.
AC   Q8RZA1; A0A0P0VCW6; Q0JG01;
DT   24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Uridine 5'-monophosphate synthase;
DE            Short=UMP synthase;
DE   Includes:
DE     RecName: Full=Orotate phosphoribosyltransferase;
DE              Short=OPRTase;
DE              EC=2.4.2.10;
DE   Includes:
DE     RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE              EC=4.1.1.23;
DE     AltName: Full=OMPdecase;
GN   Name=UMPS2; OrderedLocusNames=Os01g0951400, LOC_Os01g72250;
GN   ORFNames=B1147A04.39;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-
CC         ribose 1-diphosphate + orotate; Xref=Rhea:RHEA:10380,
CC         ChEBI:CHEBI:30839, ChEBI:CHEBI:33019, ChEBI:CHEBI:57538,
CC         ChEBI:CHEBI:58017; EC=2.4.2.10;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC         Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       UMP from orotate: step 1/2.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       UMP from orotate: step 2/2.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the purine/pyrimidine
CC       phosphoribosyltransferase family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the OMP decarboxylase
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF07327.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP003735; BAB86208.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF07327.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014957; BAS76245.1; -; Genomic_DNA.
DR   EMBL; AK102468; BAG95570.1; -; mRNA.
DR   RefSeq; XP_015621270.1; XM_015765784.1.
DR   AlphaFoldDB; Q8RZA1; -.
DR   SMR; Q8RZA1; -.
DR   STRING; 4530.OS01T0951400-01; -.
DR   PaxDb; Q8RZA1; -.
DR   PRIDE; Q8RZA1; -.
DR   EnsemblPlants; Os01t0951400-01; Os01t0951400-01; Os01g0951400.
DR   GeneID; 4325740; -.
DR   Gramene; Os01t0951400-01; Os01t0951400-01; Os01g0951400.
DR   KEGG; osa:4325740; -.
DR   eggNOG; KOG1377; Eukaryota.
DR   HOGENOM; CLU_049275_1_0_1; -.
DR   InParanoid; Q8RZA1; -.
DR   OMA; ERSEMCK; -.
DR   OrthoDB; 1303452at2759; -.
DR   UniPathway; UPA00070; UER00119.
DR   UniPathway; UPA00070; UER00120.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   ExpressionAtlas; Q8RZA1; baseline and differential.
DR   Genevisible; Q8RZA1; OS.
DR   GO; GO:0004588; F:orotate phosphoribosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IBA:GO_Central.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019856; P:pyrimidine nucleobase biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006222; P:UMP biosynthetic process; IBA:GO_Central.
DR   CDD; cd06223; PRTases_typeI; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   Gene3D; 3.40.50.2020; -; 1.
DR   HAMAP; MF_01208; PyrE; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR014732; OMPdecase.
DR   InterPro; IPR018089; OMPdecase_AS.
DR   InterPro; IPR001754; OMPdeCOase_dom.
DR   InterPro; IPR023031; OPRT.
DR   InterPro; IPR000836; PRibTrfase_dom.
DR   InterPro; IPR029057; PRTase-like.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   Pfam; PF00215; OMPdecase; 1.
DR   Pfam; PF00156; Pribosyltran; 1.
DR   SMART; SM00934; OMPdecase; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
DR   SUPFAM; SSF53271; SSF53271; 1.
DR   TIGRFAMs; TIGR01740; pyrF; 1.
DR   PROSITE; PS00156; OMPDECASE; 1.
DR   PROSITE; PS00103; PUR_PYR_PR_TRANSFER; 1.
PE   2: Evidence at transcript level;
KW   Decarboxylase; Glycosyltransferase; Lyase; Multifunctional enzyme;
KW   Pyrimidine biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..475
FT                   /note="Uridine 5'-monophosphate synthase"
FT                   /id="PRO_0000423082"
FT   ACT_SITE        305
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   475 AA;  50807 MW;  F9A503E621AEC0A0 CRC64;
     MDAAAMESLI LELHAIEAVK FGAFVLKSGI TSPIYLDLRM LVAHPRLLST VASLLGSLPA
     TRPYDLLCGV PYTALPIASV LSAAASVPML LRRYHVTPHA AAECLDGSFR AGDAVLIVED
     LVTTGSSVLE TVAPLREVGL VVADAVVVID REQGGRENLA ANGVALHSLM TLTEVLAVLV
     KRGKLGEEKA QEVKRFLDAN RKMAVPGLPV KPKVVRKAFS ERAGLATNPM GRKLFELMEA
     KQSNLCVAAD VGTATELLDL ADKIGPEICM LKTHVDILSD FTPDFGHKLR SIAERHSFLI
     FEDRKFADIG NTVTMQYEGG IFRILDWADI VNAHIVSGPG IVEGLKLKGL PKGRGLLLLS
     EMSSAGNLAH GDYTAAAVKI AEQHSDIVIG FISVNPASWS VTPSSPAFIH ATPGVQLVAG
     GDSLGQQYNT PYSVINDRGS DIIIVGRGII RANNPAETAR EYRIQGWHAY QSSLS