ID   UMPS_TOBAC              Reviewed;         461 AA.
AC   Q42942;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Uridine 5'-monophosphate synthase;
DE            Short=UMP synthase;
DE   Includes:
DE     RecName: Full=Orotate phosphoribosyltransferase;
DE              Short=OPRTase;
DE              EC=2.4.2.10;
DE   Includes:
DE     RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE              EC=4.1.1.23;
DE     AltName: Full=OMPdecase;
DE   Flags: Fragment;
GN   Name=PYR5-6;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. SR1; TISSUE=Leaf;
RA   Maier T., Zhou L., Thornburg R.W.;
RT   "Nucleotide sequence of a cDNA encoding UMP synthase from Nicotiana
RT   tabacum.";
RL   (er) Plant Gene Register PGR95-025(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-
CC         ribose 1-diphosphate + orotate; Xref=Rhea:RHEA:10380,
CC         ChEBI:CHEBI:30839, ChEBI:CHEBI:33019, ChEBI:CHEBI:57538,
CC         ChEBI:CHEBI:58017; EC=2.4.2.10;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC         Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       UMP from orotate: step 1/2.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       UMP from orotate: step 2/2.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the purine/pyrimidine
CC       phosphoribosyltransferase family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the OMP decarboxylase
CC       family. {ECO:0000305}.
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DR   EMBL; U22260; AAC49115.1; -; mRNA.
DR   PIR; T02058; T02058.
DR   AlphaFoldDB; Q42942; -.
DR   SMR; Q42942; -.
DR   STRING; 4097.Q42942; -.
DR   PRIDE; Q42942; -.
DR   UniPathway; UPA00070; UER00119.
DR   UniPathway; UPA00070; UER00120.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0004588; F:orotate phosphoribosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IBA:GO_Central.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019856; P:pyrimidine nucleobase biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006222; P:UMP biosynthetic process; IBA:GO_Central.
DR   CDD; cd06223; PRTases_typeI; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   Gene3D; 3.40.50.2020; -; 1.
DR   HAMAP; MF_01208; PyrE; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR014732; OMPdecase.
DR   InterPro; IPR018089; OMPdecase_AS.
DR   InterPro; IPR001754; OMPdeCOase_dom.
DR   InterPro; IPR023031; OPRT.
DR   InterPro; IPR004467; Or_phspho_trans_dom.
DR   InterPro; IPR000836; PRibTrfase_dom.
DR   InterPro; IPR029057; PRTase-like.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   Pfam; PF00215; OMPdecase; 1.
DR   Pfam; PF00156; Pribosyltran; 1.
DR   SMART; SM00934; OMPdecase; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
DR   SUPFAM; SSF53271; SSF53271; 1.
DR   TIGRFAMs; TIGR00336; pyrE; 1.
DR   TIGRFAMs; TIGR01740; pyrF; 1.
DR   PROSITE; PS00156; OMPDECASE; 1.
DR   PROSITE; PS00103; PUR_PYR_PR_TRANSFER; 1.
PE   2: Evidence at transcript level;
KW   Decarboxylase; Glycosyltransferase; Lyase; Multifunctional enzyme;
KW   Pyrimidine biosynthesis; Reference proteome; Transferase.
FT   CHAIN           <1..461
FT                   /note="Uridine 5'-monophosphate synthase"
FT                   /id="PRO_0000139654"
FT   ACT_SITE        291
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   461 AA;  49761 MW;  AA96479701957CD1 CRC64;
     DISAVKFGEF KLKSGISSPI YIDLRLIVSY PSILRQISQT LVGSLPSSTK YDVVCGVPYT
     ALPIATCIST AHDVPMLMRR KEVKDYGTAK AIEGAFKPGQ ACLIVEDLVT SGASVLETAA
     PLRAAGLTVT DAVVMIDREQ GGRENLAENG ITLHSMVKLT EMVKILKEKG RVSEETEKMV
     KKFLEENRKV AVPVKETKVS LRLPYQERAK IAKNPTGKKL FEIMVQKETN LCLSADVATA
     AELLDIADKV GPEICMLKTH VDILPDFTPD FGSKLRSIAD KHNFLIFEDR KFADIGNTVT
     MQYEGGIFRI LDWADITNAH IISGPGIVDG LKLKGLSRGR GLLLLAEMSS AGNLATGAYT
     AAAVKIAEDH SDFVIGFISV NPASWPNAPG NPSLIHATPG VQLVKGGDAL GQLYNTPSAV
     IADRGSDIII VGRGIIKAAN PIEAAREYRL QGWDAYLVNC K