UN130_CAEEL
ID UN130_CAEEL Reviewed; 333 AA.
AC Q18694;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Forkhead box protein unc-130 {ECO:0000305};
DE AltName: Full=Uncoordinated protein 130 {ECO:0000312|WormBase:C47G2.2};
GN Name=unc-130 {ECO:0000312|WormBase:C47G2.2};
GN ORFNames=C47G2.2 {ECO:0000312|WormBase:C47G2.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP ARG-218.
RX PubMed=11018015; DOI=10.1101/gad.832300;
RA Sarafi-Reinach T.R., Sengupta P.;
RT "The forkhead domain gene unc-130 generates chemosensory neuron diversity
RT in C. elegans.";
RL Genes Dev. 14:2472-2485(2000).
RN [3] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP ARG-218.
RX PubMed=11018016; DOI=10.1101/gad.831500;
RA Nash B., Colavita A., Zheng H., Roy P.J., Culotti J.G.;
RT "The forkhead transcription factor UNC-130 is required for the graded
RT spatial expression of the UNC-129 TGF-beta guidance factor in C. elegans.";
RL Genes Dev. 14:2486-2500(2000).
RN [4]
RP FUNCTION.
RX PubMed=22819322; DOI=10.1016/j.molcel.2012.06.021;
RA Cecere G., Zheng G.X., Mansisidor A.R., Klymko K.E., Grishok A.;
RT "Promoters recognized by forkhead proteins exist for individual 21U-RNAs.";
RL Mol. Cell 47:734-745(2012).
RN [5] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP ARG-218.
RX PubMed=27341757; DOI=10.1016/j.ydbio.2016.06.029;
RA Kersey R.K., Brodigan T.M., Fukushige T., Krause M.W.;
RT "Regulation of UNC-130/FOXD-mediated mesodermal patterning in C. elegans.";
RL Dev. Biol. 416:300-311(2016).
RN [6]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=29155044; DOI=10.1016/j.ydbio.2017.11.008;
RA Shen Q., Toulabi L.B., Shi H., Nicklow E.E., Liu J.;
RT "The forkhead transcription factor UNC-130/FOXD integrates both BMP and
RT Notch signaling to regulate dorsoventral patterning of the C. elegans
RT postembryonic mesoderm.";
RL Dev. Biol. 433:75-83(2018).
CC -!- FUNCTION: Probable transcription factor (PubMed:11018016,
CC PubMed:27341757). Binds to DNA sequence motif 5'-CTGTTTCA-3'
CC (PubMed:22819322). Required for the migration of distal tip cells (DTC)
CC and axonal growth-cones along the dorsal-ventral axis of the body wall,
CC acting by cell autonomous repression of unc-129/TGF-beta expression in
CC ventral body muscle during embyogenesis (PubMed:11018016,
CC PubMed:27341757). Binds to the promoter region of the unc-129 gene
CC (PubMed:11018016). Plays a role in dorsal-ventral patterning and fate
CC specification of the postembryonic mesoderm (PubMed:29155044). Involved
CC in male tail morphogenesis and in embryogenesis (PubMed:11018016).
CC Plays a role in the development of sensory neurons and is required to
CC repress AWA fate and promote ASG fate in the ASG chemosensory neurons
CC (PubMed:11018015). Regulates expression of a class of small RNAs, known
CC as 21U-RNAs. {ECO:0000269|PubMed:11018015, ECO:0000269|PubMed:11018016,
CC ECO:0000269|PubMed:22819322, ECO:0000269|PubMed:27341757,
CC ECO:0000269|PubMed:29155044}.
CC -!- INTERACTION:
CC Q18694; Q10666: pop-1; NbExp=3; IntAct=EBI-2414897, EBI-317870;
CC Q18694; O02482: unc-37; NbExp=3; IntAct=EBI-2414897, EBI-314716;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089,
CC ECO:0000269|PubMed:11018015}.
CC -!- TISSUE SPECIFICITY: Expressed in ventral body wall muscle
CC (PubMed:11018016). Expressed in the structural cells and two neurons of
CC each ray in the male tail (PubMed:11018016).
CC {ECO:0000269|PubMed:11018016}.
CC -!- DEVELOPMENTAL STAGE: Expressed strongly in embryos and L1 larvae, but
CC severely reduced or absent in later larval and adult stages (at the
CC protein level) (PubMed:11018015). Expressed widely in embryogenesis,
CC including ABp(l/r)aapapp cells, which are precursors to the AIB
CC interneurons and the ASI chemosensory neurons, hypodermal cells hyp4,
CC hyp5, hyp6, hyp7, H0, H1, and ABp(l/r)aapapa cells, which give rise to
CC the AWA and ASG sensory neurons (at the protein level)
CC (PubMed:11018015). Expression in the postembryonic M lineage is
CC asymmetric; beginning at the 8-M stage and continuing through the 18-M
CC stage, expressed in all the ventral, but not dorsal, M lineage cells
CC (PubMed:29155044). Expressed dynamically during embryogenesis in head
CC hypodermal cells, as well as in ventral body wall muscle (BWM) and
CC intestinal precursor cells (PubMed:11018016, PubMed:27341757).
CC Expression not detected in any dorsal BWM or its precursor during
CC embryogenesis (PubMed:27341757). {ECO:0000269|PubMed:11018015,
CC ECO:0000269|PubMed:11018016, ECO:0000269|PubMed:27341757,
CC ECO:0000269|PubMed:29155044}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown resulted in de-repression
CC of unc-129 expression in ventral body wall muscle (BWM).
CC {ECO:0000269|PubMed:27341757}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284602; CAA88935.1; -; Genomic_DNA.
DR PIR; T20033; T20033.
DR RefSeq; NP_496411.1; NM_064010.3.
DR AlphaFoldDB; Q18694; -.
DR SMR; Q18694; -.
DR IntAct; Q18694; 24.
DR STRING; 6239.C47G2.2; -.
DR EPD; Q18694; -.
DR PaxDb; Q18694; -.
DR EnsemblMetazoa; C47G2.2.1; C47G2.2.1; WBGene00006853.
DR GeneID; 174721; -.
DR KEGG; cel:CELE_C47G2.2; -.
DR UCSC; C47G2.2; c. elegans.
DR CTD; 174721; -.
DR WormBase; C47G2.2; CE02166; WBGene00006853; unc-130.
DR eggNOG; KOG2294; Eukaryota.
DR GeneTree; ENSGT00940000168146; -.
DR HOGENOM; CLU_837396_0_0_1; -.
DR InParanoid; Q18694; -.
DR OMA; MLFSMES; -.
DR OrthoDB; 1270467at2759; -.
DR SignaLink; Q18694; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00006853; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0003714; F:transcription corepressor activity; IMP:WormBase.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0048846; P:axon extension involved in axon guidance; IMP:WormBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0045185; P:maintenance of protein location; IMP:WormBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0007399; P:nervous system development; IMP:WormBase.
DR GO; GO:0042661; P:regulation of mesodermal cell fate specification; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..333
FT /note="Forkhead box protein unc-130"
FT /id="PRO_0000451993"
FT DNA_BIND 127..221
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 1..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 55..84
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..333
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 218
FT /note="R->C: In oy10 and ev582; Ectopic expression of odr-7
FT in an additional pair of cells which adopt an AWA-like
FT sensory neuron fate. Ectopic expression of unc-129 at equal
FT levels in dorsal and ventral body-wall muscle (BWM).
FT Defects in migration of distal tip cells (DTC). Does not
FT bind to unc-129 promoter in vitro."
FT /evidence="ECO:0000269|PubMed:11018015,
FT ECO:0000269|PubMed:11018016, ECO:0000269|PubMed:27341757"
SQ SEQUENCE 333 AA; 37292 MW; 79FEE50B4D665699 CRC64;
MLFSMESILS STKPKLEPPP KLEPEVTINE QVVDLPRSNT RLSEPSTSAS VLEHDLKFGE
SRKRSRSLGD EPTEDEDGVP VRKANKRNHS TSSAADSSSD DAKDDDDDDD STSRKSMSGH
RKSSHAKPPY SYIALIAMSI LNSPEKKLTL SEICEFIINK FEYYKEKFPA WQNSIRHNLS
LNDCFVKVAR GPGNPGKGNY WALDPNCEDM FDNGSFLRRR KRYKKNSDTY HEMMSHHPMP
FPPFLPQGMP FPPRMMHPMA NIPMLGHPMN PRAVPNMPAF FIPQNIDSQK LLSMMASRIM
PMDAPVSSGQ KRTSSSSSPN ENGSSAVSDK LSA
